Team:Grenoble/Biology/Protocols

From 2012.igem.org

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<section>
<section>
     <h1>Protocols</h1>
     <h1>Protocols</h1>
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     Here you can find the protocols used by the team. There are
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     In this section you will find the description of our experiments in order to be able to reproduce what we did (if you have the will). <br/>
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<br/>
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For numerous protocols, you will find a part called <span class="petit"><i><b>SAFETY AND USEFUL RECOMMANDATION</b></i></span> which is a part of our work on the <a href="https://2012.igem.org/Team:Grenoble/Safety/Assesment">safety</a>.
</section>
</section>
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     <h2>Cell culture</h2>
     <h2>Cell culture</h2>
     <ul>
     <ul>
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         <li>Medium compositions</li>
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         <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Medium">Medium composition</a></li>
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         <li>LB Agar plates preparation</li>
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         <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/LB">LB Agar plates preparation</a></li>
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         <li>Glycerol stock production</li>
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         <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Glycerol">Glycerol stock preparation</a></li>
     </ul>
     </ul>
</section>
</section>
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     <h2>BioBricks utilisation</h2>
     <h2>BioBricks utilisation</h2>
     <ul>
     <ul>
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         <li>BioBricks extraction</li>
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         <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/BB">BioBricks extraction</a></li>
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         <li>Chemically competent cells production</li>
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         <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Transformation_1">Transformation of <i>E. coli</i> cells (TSS method)</a></li>
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         <li>Transformation of <i>E. coli</i> competent cells (protocol 1)</li>
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         <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Competence">Chemically competent <i>E. coli</i> cells production</a></li>
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         <li>Transformation of <i>E. coli</i> competent cells (protocol 2)</li>
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         <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Transformation_2">Transformation of <i>E. coli</i> competent cells (heat shock)</a></li>
     </ul>
     </ul>
</section>
</section>
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     <h2>Molecular biological techniques</h2>
     <h2>Molecular biological techniques</h2>
     <ul>
     <ul>
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         <li>PCR using a GoTaq polymerase</li>
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         <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/PCR_1">PCR using a GoTaq<span class="exposant">®</span> polymerase</a></li>
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         <li>PCR using a HF Phusion polymerase</li>
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         <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/PCR_2">PCR using a HF Phusion polymerase</a></li>
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         <li>Gibson Assembly</li>
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         <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/GA">Gibson Assembly</a></li>
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         <li>Restriction enzyme digest</li>
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         <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Restriction">Restriction enzyme digestion of DNA</a></li>
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         <li>Ligation</li>
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         <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Ligation">Ligation</a></li>
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         <li>Miniprep</li>
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         <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Miniprep">Plasmid Miniprep</a></li>
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        <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Double">Double mutant construction</a></li
     </ul>
     </ul>
</section>
</section>
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     <h2>Gel migration</h2>
     <h2>Gel migration</h2>
     <ul>
     <ul>
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         <li>Gel electrophoresis</li>
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         <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Gel">Gel electrophoresis</a></li>
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         <li>DNA extraction</li>
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         <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/EtBr">Ethidium Bromide (EtBr) revelation</a></li>
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        <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/DNA_extract">DNA extraction from Agarose gel</a></li>
     </ul>
     </ul>
</section>
</section>
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     <h2>Test techniques</h2>
     <h2>Test techniques</h2>
     <ul>
     <ul>
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         <li>"AND" gate test</li>
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         <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/AND_test">"AND" gate test (control of pBAD expression by cAMP and arabinose)</a></li>
     </ul>
     </ul>
</section>
</section>

Latest revision as of 01:18, 27 September 2012

iGEM Grenoble 2012

Project

Protocols

In this section you will find the description of our experiments in order to be able to reproduce what we did (if you have the will).

For numerous protocols, you will find a part called SAFETY AND USEFUL RECOMMANDATION which is a part of our work on the safety.

Cell culture

BioBricks utilisation

Molecular biological techniques

Gel migration

Test techniques