"
Team:Grenoble/Biology/Protocols
From 2012.igem.org
(Difference between revisions)
| (30 intermediate revisions not shown) | |||
| Line 6: | Line 6: | ||
<section> | <section> | ||
<h1>Protocols</h1> | <h1>Protocols</h1> | ||
| - | + | In this section you will find the description of our experiments in order to be able to reproduce what we did (if you have the will). <br/> | |
| + | <br/> | ||
| + | For numerous protocols, you will find a part called <span class="petit"><i><b>SAFETY AND USEFUL RECOMMANDATION</b></i></span> which is a part of our work on the <a href="https://2012.igem.org/Team:Grenoble/Safety/Assesment">safety</a>. | ||
</section> | </section> | ||
<br/> | <br/> | ||
| Line 12: | Line 14: | ||
<h2>Cell culture</h2> | <h2>Cell culture</h2> | ||
<ul> | <ul> | ||
| - | <li>Medium | + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Medium">Medium composition</a></li> |
| - | <li>LB Agar plates preparation</li> | + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/LB">LB Agar plates preparation</a></li> |
| - | <li>Glycerol stock | + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Glycerol">Glycerol stock preparation</a></li> |
</ul> | </ul> | ||
</section> | </section> | ||
| Line 20: | Line 22: | ||
<h2>BioBricks utilisation</h2> | <h2>BioBricks utilisation</h2> | ||
<ul> | <ul> | ||
| - | <li>BioBricks extraction</li> | + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/BB">BioBricks extraction</a></li> |
| - | <li> | + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Transformation_1">Transformation of <i>E. coli</i> cells (TSS method)</a></li> |
| - | <li> | + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Competence">Chemically competent <i>E. coli</i> cells production</a></li> |
| - | <li>Transformation of <i>E. coli</i> competent cells ( | + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Transformation_2">Transformation of <i>E. coli</i> competent cells (heat shock)</a></li> |
</ul> | </ul> | ||
</section> | </section> | ||
<section> | <section> | ||
| - | <h2> | + | <h2>Molecular biological techniques</h2> |
| + | <ul> | ||
| + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/PCR_1">PCR using a GoTaq<span class="exposant">®</span> polymerase</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/PCR_2">PCR using a HF Phusion polymerase</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/GA">Gibson Assembly</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Restriction">Restriction enzyme digestion of DNA</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Ligation">Ligation</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Miniprep">Plasmid Miniprep</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Double">Double mutant construction</a></li | ||
| + | </ul> | ||
| + | </section> | ||
| + | <section> | ||
| + | <h2>Gel migration</h2> | ||
| + | <ul> | ||
| + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Gel">Gel electrophoresis</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/EtBr">Ethidium Bromide (EtBr) revelation</a></li> | ||
| + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/DNA_extract">DNA extraction from Agarose gel</a></li> | ||
| + | </ul> | ||
| + | </section> | ||
| + | <section> | ||
| + | <h2>Test techniques</h2> | ||
| + | <ul> | ||
| + | <li><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/AND_test">"AND" gate test (control of pBAD expression by cAMP and arabinose)</a></li> | ||
| + | </ul> | ||
| + | </section> | ||
</div> | </div> | ||
Latest revision as of 01:18, 27 September 2012
Protocols
In this section you will find the description of our experiments in order to be able to reproduce what we did (if you have the will).For numerous protocols, you will find a part called SAFETY AND USEFUL RECOMMANDATION which is a part of our work on the safety.
