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Team:Grenoble/Biology/Notebook/June/week 26
From 2012.igem.org
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<h4>Biobricks involved</h4> | <h4>Biobricks involved</h4> | ||
| - | pLAC_RBS (BBa_I13601) => final length ≃ 90bp | + | pLAC_RBS (BBa_I13601) => final length ≃ 90bp <br/> |
| - | pLAC (BBa_I13601) => final length ≃ 90bp | + | pLAC (BBa_I13601) => final length ≃ 90bp <br/> |
| - | eCFP (BBa_E0422 or BBa_E0022) => final length ≃ 800bp | + | eCFP (BBa_E0422 or BBa_E0022) => final length ≃ 800bp <br/> |
| - | plasmid pSB4K5 => final length ≃ 2400bp | + | plasmid pSB4K5 => final length ≃ 2400bp <br/> |
| - | plasmid pSB3C5 => final length ≃ 2400bp | + | plasmid pSB3C5 => final length ≃ 2400bp <br/> |
| - | plasmid pSB1A3 => final length ≃ 2400bp | + | plasmid pSB1A3 => final length ≃ 2400bp<br/> |
<h4>New parts</h4> | <h4>New parts</h4> | ||
| - | RsmA (iGEM Grenoble 2011) => final length ≃ 200bp | + | RsmA (iGEM Grenoble 2011) => final length ≃ 200bp<br/> |
| - | rsmY (iGEM Grenoble 2011) => final length ≃ 170bp | + | rsmY (iGEM Grenoble 2011) => final length ≃ 170bp<br/> |
| - | fha1 (iGEM Grenoble 2011) => final length ≃ 80bp | + | fha1 (iGEM Grenoble 2011) => final length ≃ 80bp<br/> |
Revision as of 10:07, 6 August 2012
Week 26: June 25th to July 01st
For the detection module, we decided to work on a modified membrane receptor. It consists of two merged E. coli membrane receptors. The extracellular part is the extracellular part of Tap, a dipeptide membrane receptor. The cytoplasmic part is the cytoplasmic part of the EnvZ receptor, which has the ability to activate OmpR (by phosphorilation) which is a transcription factor.
It will be a proof of concept for a bigger system which is a complete pathogene detection module :
1st network : with amplifier 1 (RsmA-rsmY)
Amplifier 1 : RsmA-rsmY system characterisation: (3 final plasmids)
Plasmid Mapping
On a pSB4K5 plasmid, we wanted to put the construction: pLAC_fha1_eCFPBiobricks involved
pLAC_RBS (BBa_I13601) => final length ≃ 90bppLAC (BBa_I13601) => final length ≃ 90bp
eCFP (BBa_E0422 or BBa_E0022) => final length ≃ 800bp
plasmid pSB4K5 => final length ≃ 2400bp
plasmid pSB3C5 => final length ≃ 2400bp
plasmid pSB1A3 => final length ≃ 2400bp
New parts
RsmA (iGEM Grenoble 2011) => final length ≃ 200bprsmY (iGEM Grenoble 2011) => final length ≃ 170bp
fha1 (iGEM Grenoble 2011) => final length ≃ 80bp
