Team:Grenoble/Biology/Notebook/July/week 33

From 2012.igem.org

(Difference between revisions)
(Created page with "{{:Team:Grenoble/Templates/Biology}} <html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en"> <body id="Biology"> <section> <h1>August</h1> a href="http://2012.igem....")
Line 4: Line 4:
<body id="Biology">
<body id="Biology">
 +
<div id="cadre">
<section>
<section>
<h1>August</h1>
<h1>August</h1>

Revision as of 10:51, 26 September 2012

iGEM Grenoble 2012

Project

August

a href="https://2012.igem.org/Team:Grenoble/Biology/Notebook/August">Week 31 • Week 32Week 33Week 34Week 35

Week 33: August 13th to 19th

Goal of the week:

Assembly of:
  • pAra/Bad_RBS_GFP and RBS_Cya.
  • pompC and mcherry
  • Tap and EnvZ

Monday, August 13th:

We did some verification PCRs on miniprep with HF Phusion enzyme (protocol) in order to check the Gibson Assembly products (12/09/09>.
Annealing temperature = 55°C.

To separate (protocol) the PCR products, we prepared a 1.8% TAE agarose gel.
Migration conditions = 100V during 30 min.
In order to reveal the DNA fragments, we used EtBr.

It showed no significant results (data not shown).

Tuesday, August 14th:

We did an experiment (protocol) in order to test the "AND" gate (CRP-cAMP & araC) without the amplifier module.

We transformed (new protocol) BW25113 WT competent cells (protocol) with pSB3C5.