Team:Grenoble/Biology/Notebook/July/week 33

From 2012.igem.org

(Difference between revisions)
(Blanked the page)
 
Line 1: Line 1:
-
{{:Team:Grenoble/Templates/Biology}}
 
-
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en">
 
-
<body id="Biology">
 
-
 
-
<div id="cadre">
 
-
<section>
 
-
<h1>August</h1>
 
-
<a href="https://2012.igem.org/Team:Grenoble/Biology/Notebook/August">Week 31</a> •
 
-
<a href="https://2012.igem.org/Team:Grenoble/Biology/Notebook/August/week_32">Week 32</a> •
 
-
<a href="https://2012.igem.org/Team:Grenoble/Biology/Notebook/August/week_33">Week 33</a> •
 
-
<a href="https://2012.igem.org/Team:Grenoble/Biology/Notebook/August/week_34">Week 34</a> •
 
-
<a href="https://2012.igem.org/Team:Grenoble/Biology/Notebook/August/week_35">Week 35</a>
 
-
</section>
 
-
<br/>
 
-
<section>
 
-
<h1> Week 33: August 13<span class="exposant">th</span> to 19<span class="exposant">th</span> </h1>
 
-
<h2> Goal of the week: </h2>
 
-
Assembly of:
 
-
<ul><li>pAra/Bad_RBS_GFP and RBS_Cya.</li>
 
-
    <li>pompC and mcherry</li>
 
-
    <li>Tap and EnvZ</li>
 
-
</ul>
 
-
</section>
 
-
 
-
<section>
 
-
    <h2> Monday, August 13<span class="exposant">th</span>:</h2>
 
-
We did some verification PCRs on miniprep with HF Phusion enzyme (<a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/PCR_2">protocol</a>) in order to check the
 
-
Gibson Assembly products (12/09/09>.<br/>Annealing temperature = 55°C.<br/>
 
-
<br/>
 
-
To separate (<a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Gel">protocol</a>) the PCR products, we prepared a 1.8% TAE agarose gel.<br/>
 
-
Migration conditions = 100V during 30 min.<br/>
 
-
In order to reveal the DNA fragments, we used <a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/EtBr">EtBr</a>.<br/>
 
-
<br/>
 
-
It showed no significant results (data not shown).
 
-
</section>
 
-
 
-
<section>
 
-
    <h2> Tuesday, August 14<span class="exposant">th</span>:</h2>
 
-
    We did an experiment (<a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/AND_test">protocol</a>) in order to test the "AND" gate (CRP-cAMP & araC) without the amplifier module. <br/>
 
-
    <br/>
 
-
    We transformed (new <a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Transformation_2">protocol</a>) BW25113 WT
 
-
competent cells (<a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Competence">protocol</a>) with pSB3C5.<br/>
 
-
</section>
 
-
 
-
<section>
 
-
    <h2> Wednesday, August 15<span class="exposant">th</span>:</h2>
 
-
    We did an experiment (<a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/AND_test">protocol</a>) in order to test the "AND" gate (CRP-cAMP & araC) without the amplifier module. The M9 medium used was complemented: 0.1% acetate.
 
-
</section>
 
-
 
-
<section>
 
-
    <h2>Thursday, August 16<span class="exposant">th</span>:</h2>
 
-
We did some colony PCRs with HF Phusion enzyme (<a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/PCR_2">protocol</a>) in order to amplify pSB1A3 (araC), pSB3C5 (araC), pSB3C5 (pAra/Bad_RBS_GFP_RBS_Cya) and pSB4C5 (pAra/Bad_RBS_GFP_RBS_Cya)<br/>
 
-
<br/>
 
-
Then, we did some digestions (<a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Restriction">protocol</a>). The digestions were achieved with two restriction enzymes:
 
-
<ul>
 
-
<li> pOmpC with EcoRI and SpeI during 10 minutes </li>
 
-
<li> pOmpC with EcoRI</li>
 
-
<li> pSB4K5 with EcoRI and SpeI during 10 minutes</li>
 
-
<li> pSB4K5 with EcoRI during 10 minutes</li>
 
-
</ul>
 
-
<br/>
 
-
To separate (<a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Gel">protocol</a>) the PCR and the digestion products, we prepared a 1.8% TAE agarose gel.<br/>
 
-
Migration conditions = 100V during 30 min.<br/>
 
-
In order to reveal the DNA fragments, we used <a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/EtBr">EtBr</a>.<br/>
 
-
<br/>
 
-
The PCR and the digestion worked well, we realised a DNA extraction (<a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/DNA_extract">protocol</a>) from the pSB1A3 (araC) and pSB4C5 (pAra/Bad_RBS_GFP_RBS_Cya) PCR products.<br/>
 
-
<br/>
 
-
 
-
 
-
 
-
</div>
 
-
</body>
 
-
</html>
 
-
{{:Team:Grenoble/script}}
 
-
{{:Team:Grenoble/menu}}
 

Latest revision as of 22:54, 26 September 2012