Team:Grenoble/Biology/Notebook/July/week 32

From 2012.igem.org

(Difference between revisions)
Adeline millet (Talk | contribs)
(Created page with "{{:Team:Grenoble/Templates/Biology}} <html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en"> <body id="Biology"> <section> <h1>August</h1> <a href="http://2012.igem...")
Newer edit →

Revision as of 08:28, 20 August 2012

iGEM Grenoble 2012

iGEM Grenoble iGEM
iGEM 2012
main page
Project

August

Week 31 • Week 32 • Week 33 • Week 34 • Week 35

Week 32: August 06th to 12th

Goal of the week:

Monday, August 06th:

To separate the miniprep (12/08/03) products, we prepared a 1.8% TAE agarose gel.
Migration conditions = 100V during 30 min.
In order to reveal the DNA fragments, we used EtBr.

photo_gel_27
Migration result for a 1.8% TAE agarose gel
(the DNA ladder scale is in kb)
Lane 1: pLAC_rsmY (pSB1A3) 1 miniprep product
Lane 2: pLAC_rsmY (pSB1A3) 2 miniprep product
Lane 3: pLAC_rsmY (pSB1A3) 3 miniprep product
Lane 4: pLAC_fha1_eCFP (pSB4C5) 1 miniprep product
Lane 5: pLAC_fha1_eCFP (pSB4C5) 2 miniprep product
Lane 6: DNA ladder 1kb (biolabs)
Lane 7: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 1 miniprep product
Lane 8: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 2 miniprep product
Lane 9: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 1 miniprep product
Lane 10: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 2 miniprep product
Lane 11: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 3 miniprep product
Lane 12: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 4 miniprep product
Lane 13: DNA ladder 1kb (biolabs)

Retrieved from "http://2012.igem.org/Team:Grenoble/Biology/Notebook/July/week_32"