Team:Grenoble/Biology/Notebook/July/week 32

From 2012.igem.org

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<a href="http://2012.igem.org/Team:Grenoble/Biology/Notebook/July/week_34">Week 34</a> •  
<a href="http://2012.igem.org/Team:Grenoble/Biology/Notebook/July/week_34">Week 34</a> •  
<a href="http://2012.igem.org/Team:Grenoble/Biology/Notebook/July/week_35">Week 35</a>
<a href="http://2012.igem.org/Team:Grenoble/Biology/Notebook/July/week_35">Week 35</a>
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<h1> Week 32: August 06<span class="exposant">th</span> to 12<span class="exposant">th</span> </h1>
<h1> Week 32: August 06<span class="exposant">th</span> to 12<span class="exposant">th</span> </h1>
<h2> Goal of the week: </h2>
<h2> Goal of the week: </h2>
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<h2> Monday, August 06<span class="exposant">th</span>:</h2>
<h2> Monday, August 06<span class="exposant">th</span>:</h2>
We wanted to check if the Gibson Assemblies (12/08/01) worked well.<br/>
We wanted to check if the Gibson Assemblies (12/08/01) worked well.<br/>
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Revision as of 13:01, 20 August 2012

iGEM Grenoble 2012

Project

August

Week 31Week 32Week 33Week 34Week 35


Week 32: August 06th to 12th

Goal of the week:


Monday, August 06th:

We wanted to check if the Gibson Assemblies (12/08/01) worked well.

To separate the GA miniprep (12/08/03) products, we prepared a 1.8% TAE agarose gel.
Migration conditions = 100V during 30 min.
In order to reveal the DNA fragments, we used EtBr.

photo_gel_27
Migration result for a 1.8% TAE agarose gel
(the DNA ladder scale is in kb)
Lane 1: pLAC_rsmY (pSB1A3) 3 miniprep product
Lane 2: pLAC_rsmY (pSB1A3) 2 miniprep product
Lane 3: pLAC_rsmY (pSB1A3) 1 miniprep product
Lane 4: pLAC_fha1_eCFP (pSB4C5) 2 miniprep product
Lane 5: pLAC_fha1_eCFP (pSB4C5) 1 miniprep product
Lane 6: DNA ladder 1kb (biolabs)
Lane 7: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 1 miniprep product
Lane 8: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 2 miniprep product
Lane 9: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 4 miniprep product
Lane 10: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 3 miniprep product
Lane 11: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 1 miniprep product
Lane 12: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 2 miniprep product
Lane 13: DNA ladder 1kb (biolabs)


We did some digestions (protocol) on GA miniprep (12/08/03). The digestions were achieved with XbaI during 10 minutes.

To separate the digestion products, we prepared two 1.8% TAE agarose gel.
Migration conditions = 100V during 30 min.
In order to reveal the DNA fragments, we used EtBr.

photo_gel_28
Migration result for a 1.8% TAE agarose gel
(the DNA ladder scale is in kb)
Lane 1: data not shown pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 1 digestion product
Lane 2: data not shown pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 1 miniprep product
Lane 3: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 2 digestion product
Lane 4: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 2 miniprep product
Lane 5: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 4 digestion product
Lane 6: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 4 miniprep product
Lane 7: DNA ladder 1kb (biolabs)
Lane 8: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 3 digestion product
Lane 9: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 3 miniprep product
Lane 10: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 1 digestion product
Lane 11: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 1 miniprep product
Lane 12: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 2 digestion product
Lane 13: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 2 miniprep product



photo_gel_29
Migration result for a 1.8% TAE agarose gel
(the DNA ladder scale is in kb)
Lane 1: DNA ladder 1kb (biolabs)
Lane 2: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 4 digestion product
Lane 3: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 4 miniprep product
Lane 4: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 1 digestion product
Lane 5: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 1 miniprep product
Lane 6: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 2 digestion product
Lane 7: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 2 miniprep product
Lane 8:DNA ladder 80pb-10kb (fermentas)