Team:Grenoble/Biology/Notebook/July/week 32

From 2012.igem.org

(Difference between revisions)
Line 26: Line 26:
<div class="legend"><u>Migration result for a 1.8% TAE agarose gel</u><br/>
<div class="legend"><u>Migration result for a 1.8% TAE agarose gel</u><br/>
   <i>(the DNA ladder scale is in kb)</i><br/>
   <i>(the DNA ladder scale is in kb)</i><br/>
-
Lane 1: pLAC_rsmY (pSB1A3) <b>1</b> miniprep product<br/>
+
Lane 1: pLAC_rsmY (pSB1A3) <b>3</b> miniprep product<br/>
Lane 2: pLAC_rsmY (pSB1A3) <b>2</b> miniprep product<br/>
Lane 2: pLAC_rsmY (pSB1A3) <b>2</b> miniprep product<br/>
-
Lane 3: pLAC_rsmY (pSB1A3) <b>3</b> miniprep product<br/>
+
Lane 3: pLAC_rsmY (pSB1A3) <b>1</b> miniprep product<br/>
-
Lane 4: pLAC_fha1_eCFP (pSB4C5) <b>1</b> miniprep product<br/>
+
Lane 4: pLAC_fha1_eCFP (pSB4C5) <b>2</b> miniprep product<br/>
-
Lane 5: pLAC_fha1_eCFP (pSB4C5) <b>2</b> miniprep product<br/>
+
Lane 5: pLAC_fha1_eCFP (pSB4C5) <b>1</b> miniprep product<br/>
Lane 6: DNA ladder 1kb (biolabs)<br/>
Lane 6: DNA ladder 1kb (biolabs)<br/>
Lane 7: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) <b>1</b> miniprep product<br/>
Lane 7: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) <b>1</b> miniprep product<br/>
Lane 8: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) <b>2</b> miniprep product<br/>
Lane 8: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) <b>2</b> miniprep product<br/>
-
Lane 9: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>1</b> miniprep product<br/>
+
Lane 9: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>4</b> miniprep product<br/>
-
Lane 10: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>2</b> miniprep product<br/>
+
Lane 10: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>3</b> miniprep product<br/>
-
Lane 11: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>3</b> miniprep product<br/>
+
Lane 11: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>1</b> miniprep product<br/>
-
Lane 12: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>4</b> miniprep product<br/>
+
Lane 12: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>2</b> miniprep product<br/>
Lane 13: DNA ladder 1kb (biolabs)<br/></div>
Lane 13: DNA ladder 1kb (biolabs)<br/></div>
 +
<br/>
<br/>
<br/>
We did some digestions (protocol) on GA miniprep (12/08/03). The digestions were achieved with XbaI during 10 minutes.<br/>
We did some digestions (protocol) on GA miniprep (12/08/03). The digestions were achieved with XbaI during 10 minutes.<br/>
Line 53: Line 54:
Lane 3: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) <b>2</b> digestion product<br/>
Lane 3: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) <b>2</b> digestion product<br/>
Lane 4: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) <b>2</b> miniprep product<br/>
Lane 4: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) <b>2</b> miniprep product<br/>
-
Lane 5: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>1</b> digestion product<br/>
+
Lane 5: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>4</b> digestion product<br/>
-
Lane 6: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>1</b> miniprep product<br/>
+
Lane 6: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>4</b> miniprep product<br/>
Lane 7: DNA ladder 1kb (biolabs)<br/>
Lane 7: DNA ladder 1kb (biolabs)<br/>
-
Lane 8: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>2</b> digestion product<br/>
+
Lane 8: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>3</b> digestion product<br/>
-
Lane 9: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>2</b> miniprep product<br/>
+
Lane 9: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>3</b> miniprep product<br/>
-
Lane 10: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>3</b> digestion product<br/>
+
Lane 10: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>1</b> digestion product<br/>
-
Lane 11: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>3</b> miniprep product<br/>
+
Lane 11: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>1</b> miniprep product<br/>
-
Lane 12: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>4</b> digestion product<br/>
+
Lane 12: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>2</b> digestion product<br/>
-
Lane 13: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>4</b> miniprep product<br/></div>
+
Lane 13: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>2</b> miniprep product<br/></div>
 +
<br/>
 +
 
 +
<br/>
<br/>
<br/>
<center><img src="http://2012.igem.org/wiki/images/3/3e/120806.jpg" alt="photo_gel_29"/></center>
<center><img src="http://2012.igem.org/wiki/images/3/3e/120806.jpg" alt="photo_gel_29"/></center>
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   <i>(the DNA ladder scale is in kb)</i><br/>
   <i>(the DNA ladder scale is in kb)</i><br/>
Lane 1: DNA ladder 1kb (biolabs)<br/>
Lane 1: DNA ladder 1kb (biolabs)<br/>
-
Lane 2: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>1</b> digestion product<br/>
+
Lane 2: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>4</b> digestion product<br/>
-
Lane 3: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>1</b> miniprep product<br/>
+
Lane 3: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>4</b> miniprep product<br/>
-
Lane 4: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>2</b> digestion product<br/>
+
Lane 4: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>1</b> digestion product<br/>
-
Lane 5: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>2</b> miniprep product<br/>
+
Lane 5: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>1</b> miniprep product<br/>
-
Lane 6: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>3</b> digestion product<br/>
+
Lane 6: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>2</b> digestion product<br/>
-
Lane 7: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>3</b> miniprep product<br/>
+
Lane 7: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>2</b> miniprep product<br/>
Lane 8:DNA ladder 80pb-10kb (fermentas)<br/>
Lane 8:DNA ladder 80pb-10kb (fermentas)<br/>
</div>
</div>

Revision as of 09:46, 20 August 2012

iGEM Grenoble 2012

Project

August

Week 31Week 32Week 33Week 34Week 35

Week 32: August 06th to 12th

Goal of the week:

Monday, August 06th:

We wanted to check if the Gibson Assemblies (12/08/01) worked well.

To separate the GA miniprep (12/08/03) products, we prepared a 1.8% TAE agarose gel.
Migration conditions = 100V during 30 min.
In order to reveal the DNA fragments, we used EtBr.

photo_gel_27
Migration result for a 1.8% TAE agarose gel
(the DNA ladder scale is in kb)
Lane 1: pLAC_rsmY (pSB1A3) 3 miniprep product
Lane 2: pLAC_rsmY (pSB1A3) 2 miniprep product
Lane 3: pLAC_rsmY (pSB1A3) 1 miniprep product
Lane 4: pLAC_fha1_eCFP (pSB4C5) 2 miniprep product
Lane 5: pLAC_fha1_eCFP (pSB4C5) 1 miniprep product
Lane 6: DNA ladder 1kb (biolabs)
Lane 7: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 1 miniprep product
Lane 8: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 2 miniprep product
Lane 9: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 4 miniprep product
Lane 10: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 3 miniprep product
Lane 11: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 1 miniprep product
Lane 12: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 2 miniprep product
Lane 13: DNA ladder 1kb (biolabs)


We did some digestions (protocol) on GA miniprep (12/08/03). The digestions were achieved with XbaI during 10 minutes.

To separate the digestion products, we prepared two 1.8% TAE agarose gel.
Migration conditions = 100V during 30 min.
In order to reveal the DNA fragments, we used EtBr.

photo_gel_28
Migration result for a 1.8% TAE agarose gel
(the DNA ladder scale is in kb)
Lane 1: data not shown pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 1 digestion product
Lane 2: data not shown pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 1 miniprep product
Lane 3: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 2 digestion product
Lane 4: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 2 miniprep product
Lane 5: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 4 digestion product
Lane 6: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 4 miniprep product
Lane 7: DNA ladder 1kb (biolabs)
Lane 8: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 3 digestion product
Lane 9: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 3 miniprep product
Lane 10: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 1 digestion product
Lane 11: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 1 miniprep product
Lane 12: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 2 digestion product
Lane 13: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 2 miniprep product



photo_gel_29
Migration result for a 1.8% TAE agarose gel
(the DNA ladder scale is in kb)
Lane 1: DNA ladder 1kb (biolabs)
Lane 2: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 4 digestion product
Lane 3: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 4 miniprep product
Lane 4: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 1 digestion product
Lane 5: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 1 miniprep product
Lane 6: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 2 digestion product
Lane 7: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 2 miniprep product
Lane 8:DNA ladder 80pb-10kb (fermentas)