Team:Grenoble/Biology/Notebook/July/week 32
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<h2> Monday, August 06<span class="exposant">th</span>:</h2> | <h2> Monday, August 06<span class="exposant">th</span>:</h2> | ||
- | To separate the miniprep (12/08/03) products, we prepared a 1.8% TAE agarose gel.<br/> | + | We wanted to check if the Gibson Assemblies (12/08/01) worked well.<br/> |
+ | <br/> | ||
+ | To separate the GA miniprep (12/08/03) products, we prepared a 1.8% TAE agarose gel.<br/> | ||
Migration conditions = 100V during 30 min.<br/> | Migration conditions = 100V during 30 min.<br/> | ||
In order to reveal the DNA fragments, we used EtBr.<br/> | In order to reveal the DNA fragments, we used EtBr.<br/> | ||
Line 38: | Line 40: | ||
Lane 13: DNA ladder 1kb (biolabs)<br/></div> | Lane 13: DNA ladder 1kb (biolabs)<br/></div> | ||
<br/> | <br/> | ||
- | + | We did some digestions (protocol) on GA miniprep (12/08/03). The digestions were achieved with XbaI during 10 minutes.<br/> | |
+ | <br/> | ||
+ | To separate the digestion products, we prepared two 1.8% TAE agarose gel.<br/> | ||
+ | Migration conditions = 100V during 30 min.<br/> | ||
+ | In order to reveal the DNA fragments, we used EtBr.<br/> | ||
+ | <br/> | ||
+ | <center><img src="https://static.igem.org/mediawiki/2012/c/cd/120806_dig2.jpg" alt="photo_gel_28"/></center> | ||
+ | <div class="legend"><u>Migration result for a 1.8% TAE agarose gel</u><br/> | ||
+ | <i>(the DNA ladder scale is in kb)</i><br/> | ||
+ | Lane 1: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) <b>1</b> digestion product<br/> | ||
+ | Lane 2: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) <b>1</b> miniprep product<br/> | ||
+ | Lane 3: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) <b>2</b> digestion product<br/> | ||
+ | Lane 4: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) <b>2</b> miniprep product<br/> | ||
+ | Lane 5: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>1</b> digestion product<br/> | ||
+ | Lane 6: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>1</b> miniprep product<br/> | ||
+ | Lane 7: DNA ladder 1kb (biolabs)<br/> | ||
+ | Lane 8: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>2</b> digestion product<br/> | ||
+ | Lane 9: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>2</b> miniprep product<br/> | ||
+ | Lane 10: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>3</b> digestion product<br/> | ||
+ | Lane 11: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>3</b> miniprep product<br/> | ||
+ | Lane 12: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>4</b> digestion product<br/> | ||
+ | Lane 13: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>4</b> miniprep product<br/></div> | ||
+ | <br/> | ||
+ | <center><img src="https://static.igem.org/mediawiki/2012/3/3e/120806.jpg" alt="photo_gel_29"/></center> | ||
+ | <div class="legend"><u>Migration result for a 1.8% TAE agarose gel</u><br/> | ||
+ | <i>(the DNA ladder scale is in kb)</i><br/> | ||
+ | Lane 1: DNA ladder 1kb (biolabs)<br/> | ||
+ | Lane 2: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>1</b> digestion product<br/> | ||
+ | Lane 3: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>1</b> miniprep product<br/> | ||
+ | Lane 4: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>2</b> digestion product<br/> | ||
+ | Lane 5: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>2</b> miniprep product<br/> | ||
+ | Lane 6: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>3</b> digestion product<br/> | ||
+ | Lane 7: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>3</b> miniprep product<br/> | ||
+ | Lane 8:DNA ladder 80pb-10kb (fermentas)<br/> | ||
+ | </div> | ||
+ | <br/> | ||
</section> | </section> | ||
</body> | </body> |
Revision as of 09:08, 20 August 2012
August
Week 31 • Week 32 • Week 33 • Week 34 • Week 35Week 32: August 06th to 12th
Goal of the week:
Monday, August 06th:
We wanted to check if the Gibson Assemblies (12/08/01) worked well.To separate the GA miniprep (12/08/03) products, we prepared a 1.8% TAE agarose gel.
Migration conditions = 100V during 30 min.
In order to reveal the DNA fragments, we used EtBr.
Migration result for a 1.8% TAE agarose gel
(the DNA ladder scale is in kb)
Lane 1: pLAC_rsmY (pSB1A3) 1 miniprep product
Lane 2: pLAC_rsmY (pSB1A3) 2 miniprep product
Lane 3: pLAC_rsmY (pSB1A3) 3 miniprep product
Lane 4: pLAC_fha1_eCFP (pSB4C5) 1 miniprep product
Lane 5: pLAC_fha1_eCFP (pSB4C5) 2 miniprep product
Lane 6: DNA ladder 1kb (biolabs)
Lane 7: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 1 miniprep product
Lane 8: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 2 miniprep product
Lane 9: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 1 miniprep product
Lane 10: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 2 miniprep product
Lane 11: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 3 miniprep product
Lane 12: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 4 miniprep product
Lane 13: DNA ladder 1kb (biolabs)
(the DNA ladder scale is in kb)
Lane 1: pLAC_rsmY (pSB1A3) 1 miniprep product
Lane 2: pLAC_rsmY (pSB1A3) 2 miniprep product
Lane 3: pLAC_rsmY (pSB1A3) 3 miniprep product
Lane 4: pLAC_fha1_eCFP (pSB4C5) 1 miniprep product
Lane 5: pLAC_fha1_eCFP (pSB4C5) 2 miniprep product
Lane 6: DNA ladder 1kb (biolabs)
Lane 7: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 1 miniprep product
Lane 8: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 2 miniprep product
Lane 9: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 1 miniprep product
Lane 10: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 2 miniprep product
Lane 11: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 3 miniprep product
Lane 12: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 4 miniprep product
Lane 13: DNA ladder 1kb (biolabs)
We did some digestions (protocol) on GA miniprep (12/08/03). The digestions were achieved with XbaI during 10 minutes.
To separate the digestion products, we prepared two 1.8% TAE agarose gel.
Migration conditions = 100V during 30 min.
In order to reveal the DNA fragments, we used EtBr.
Migration result for a 1.8% TAE agarose gel
(the DNA ladder scale is in kb)
Lane 1: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 1 digestion product
Lane 2: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 1 miniprep product
Lane 3: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 2 digestion product
Lane 4: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 2 miniprep product
Lane 5: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 1 digestion product
Lane 6: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 1 miniprep product
Lane 7: DNA ladder 1kb (biolabs)
Lane 8: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 2 digestion product
Lane 9: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 2 miniprep product
Lane 10: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 3 digestion product
Lane 11: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 3 miniprep product
Lane 12: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 4 digestion product
Lane 13: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 4 miniprep product
(the DNA ladder scale is in kb)
Lane 1: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 1 digestion product
Lane 2: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 1 miniprep product
Lane 3: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 2 digestion product
Lane 4: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 2 miniprep product
Lane 5: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 1 digestion product
Lane 6: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 1 miniprep product
Lane 7: DNA ladder 1kb (biolabs)
Lane 8: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 2 digestion product
Lane 9: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 2 miniprep product
Lane 10: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 3 digestion product
Lane 11: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 3 miniprep product
Lane 12: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 4 digestion product
Lane 13: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 4 miniprep product
Migration result for a 1.8% TAE agarose gel
(the DNA ladder scale is in kb)
Lane 1: DNA ladder 1kb (biolabs)
Lane 2: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 1 digestion product
Lane 3: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 1 miniprep product
Lane 4: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 2 digestion product
Lane 5: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 2 miniprep product
Lane 6: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 3 digestion product
Lane 7: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 3 miniprep product
Lane 8:DNA ladder 80pb-10kb (fermentas)
(the DNA ladder scale is in kb)
Lane 1: DNA ladder 1kb (biolabs)
Lane 2: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 1 digestion product
Lane 3: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 1 miniprep product
Lane 4: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 2 digestion product
Lane 5: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 2 miniprep product
Lane 6: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 3 digestion product
Lane 7: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 3 miniprep product
Lane 8:DNA ladder 80pb-10kb (fermentas)