Team:Grenoble/Biology/Notebook/July/week 32

From 2012.igem.org

(Difference between revisions)
(Blanked the page)
 
(7 intermediate revisions not shown)
Line 1: Line 1:
-
{{:Team:Grenoble/Templates/Biology}}
 
-
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en">
 
-
<body id="Biology">
 
-
 
-
<div id="cadre">
 
-
<section>
 
-
<h1>August</h1>
 
-
<a href="https://2012.igem.org/Team:Grenoble/Biology/Notebook/August">Week 31</a> •
 
-
<a href="https://2012.igem.org/Team:Grenoble/Biology/Notebook/July/week_32">Week 32</a> •
 
-
<a href="https://2012.igem.org/Team:Grenoble/Biology/Notebook/July/week_33">Week 33</a> •
 
-
<a href="https://2012.igem.org/Team:Grenoble/Biology/Notebook/July/week_34">Week 34</a> •
 
-
<a href="https://2012.igem.org/Team:Grenoble/Biology/Notebook/July/week_35">Week 35</a>
 
-
</section>
 
-
<br/>
 
-
<section>
 
-
<h1> Week 32: August 06<span class="exposant">th</span> to 12<span class="exposant">th</span> </h1>
 
-
<h2> Goal of the week: </h2>
 
-
</section>
 
-
<section>
 
-
<h2> Monday, August 06<span class="exposant">th</span>:</h2>
 
-
We wanted to check if the Gibson Assemblies (12/08/01) worked well.<br/>
 
-
<br/>
 
-
To separate the GA miniprep (12/08/03) products, we prepared a 1.8% TAE agarose gel.<br/>
 
-
Migration conditions = 100V during 30 min.<br/>
 
-
In order to reveal the DNA fragments, we used EtBr.<br/>
 
-
<br/>
 
-
<center><img src="https://static.igem.org/mediawiki/2012/4/44/120806_pcr_miniprep_2.jpg" alt="photo_gel_27"/></center>
 
-
<div class="legend"><p><u>Migration result for a 1.8% TAE agarose gel</u><br/>
 
-
  <i>(the DNA ladder scale is in kb)</i></p>
 
-
<ul><li><b>Lane 1:</b> pLAC_rsmY (pSB1A3) <b>3</b> miniprep product</li>
 
-
<li><b>Lane 2:</b> pLAC_rsmY (pSB1A3) <b>2</b> miniprep product</li>
 
-
<li><b>Lane 3:</b> pLAC_rsmY (pSB1A3) <b>1</b> miniprep product</li>
 
-
<li><b>Lane 4:</b> pLAC_fha1_eCFP (pSB4C5) <b>2</b> miniprep product</li>
 
-
<li><b>Lane 5:</b> pLAC_fha1_eCFP (pSB4C5) <b>1</b> miniprep product</li>
 
-
<li><b>Lane 6:</b> DNA ladder 1kb (biolabs)</li>
 
-
<li><b>Lane 7:</b> pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) <b>1</b> miniprep product</li>
 
-
<li><b>Lane 8:</b> pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) <b>2</b> miniprep product</li>
 
-
<li><b>Lane 9:</b> pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>4</b> miniprep product</li>
 
-
<li><b>Lane 10:</b> pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>3</b> miniprep product</li>
 
-
<li><b>Lane 11:</b> pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>1</b> miniprep product</li>
 
-
<li><b>Lane 12:</b> pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>2</b> miniprep product</li>
 
-
<li><b>Lane 13:</b> DNA ladder 1kb (biolabs)</li></div>
 
-
<br/>
 
-
<br/>
 
-
We did some digestions (protocol) on GA miniprep (12/08/03). The digestions were achieved with XbaI during 10 minutes.<br/>
 
-
<br/>
 
-
To separate the digestion products, we prepared two 1.8% TAE agarose gel.<br/>
 
-
Migration conditions = 100V during 30 min.<br/>
 
-
In order to reveal the DNA fragments, we used EtBr.<br/>
 
-
<br/>
 
-
<center><img src="https://static.igem.org/mediawiki/2012/c/cd/120806_dig2.jpg" alt="photo_gel_28"/></center>
 
-
<div class="legend"><u>Migration result for a 1.8% TAE agarose gel</u><br/>
 
-
  <i>(the DNA ladder scale is in kb)</i><br/>
 
-
Lane 1: data not shown pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) <b>1</b> digestion product<br/>
 
-
Lane 2: data not shown pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) <b>1</b> miniprep product<br/>
 
-
Lane 3: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) <b>2</b> digestion product<br/>
 
-
Lane 4: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) <b>2</b> miniprep product<br/>
 
-
Lane 5: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>4</b> digestion product<br/>
 
-
Lane 6: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>4</b> miniprep product<br/>
 
-
Lane 7: DNA ladder 1kb (biolabs)<br/>
 
-
Lane 8: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>3</b> digestion product<br/>
 
-
Lane 9: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>3</b> miniprep product<br/>
 
-
Lane 10: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>1</b> digestion product<br/>
 
-
Lane 11: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>1</b> miniprep product<br/>
 
-
Lane 12: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>2</b> digestion product<br/>
 
-
Lane 13: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>2</b> miniprep product<br/></div>
 
-
<br/>
 
-
 
-
<br/>
 
-
<br/>
 
-
<center><img src="https://static.igem.org/mediawiki/2012/3/3e/120806.jpg" alt="photo_gel_29"/></center>
 
-
<div class="legend"><u>Migration result for a 1.8% TAE agarose gel</u><br/>
 
-
  <i>(the DNA ladder scale is in kb)</i><br/>
 
-
Lane 1: DNA ladder 1kb (biolabs)<br/>
 
-
Lane 2: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>4</b> digestion product<br/>
 
-
Lane 3: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>4</b> miniprep product<br/>
 
-
Lane 4: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>1</b> digestion product<br/>
 
-
Lane 5: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>1</b> miniprep product<br/>
 
-
Lane 6: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>2</b> digestion product<br/>
 
-
Lane 7: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>2</b> miniprep product<br/>
 
-
Lane 8:DNA ladder 80pb-10kb (fermentas)<br/>
 
-
</div>
 
-
</section>
 
-
<section>
 
-
</div>
 
-
</body>
 
-
</html>
 
-
{{:Team:Grenoble/script}}
 
-
{{:Team:Grenoble/menu}}
 

Latest revision as of 09:58, 26 September 2012