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- | {{:Team:Grenoble/Templates/Biology}}
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- | <html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en">
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- | <body id="Biology">
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- | <section>
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- | <h1>August</h1>
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- | <a href="https://2012.igem.org/Team:Grenoble/Biology/Notebook/August">Week 31</a> •
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- | <a href="https://2012.igem.org/Team:Grenoble/Biology/Notebook/July/week_32">Week 32</a> •
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- | <a href="https://2012.igem.org/Team:Grenoble/Biology/Notebook/July/week_33">Week 33</a> •
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- | <a href="https://2012.igem.org/Team:Grenoble/Biology/Notebook/July/week_34">Week 34</a> •
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- | <a href="https://2012.igem.org/Team:Grenoble/Biology/Notebook/July/week_35">Week 35</a>
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- | <br/>
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- | <br/>
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- | <h1> Week 32: August 06<span class="exposant">th</span> to 12<span class="exposant">th</span> </h1>
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- | <h2> Goal of the week: </h2>
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- | <h2> Monday, August 06<span class="exposant">th</span>:</h2>
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- | We wanted to check if the Gibson Assemblies (12/08/01) worked well.<br/>
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- | <br/>
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- | To separate the GA miniprep (12/08/03) products, we prepared a 1.8% TAE agarose gel.<br/>
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- | Migration conditions = 100V during 30 min.<br/>
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- | In order to reveal the DNA fragments, we used EtBr.<br/>
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- | <br/>
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- | <center><img src="https://static.igem.org/mediawiki/2012/4/44/120806_pcr_miniprep_2.jpg" alt="photo_gel_27"/></center>
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- | <div class="legend"><u>Migration result for a 1.8% TAE agarose gel</u><br/>
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- | <i>(the DNA ladder scale is in kb)</i><br/>
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- | Lane 1: pLAC_rsmY (pSB1A3) <b>1</b> miniprep product<br/>
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- | Lane 2: pLAC_rsmY (pSB1A3) <b>2</b> miniprep product<br/>
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- | Lane 3: pLAC_rsmY (pSB1A3) <b>3</b> miniprep product<br/>
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- | Lane 4: pLAC_fha1_eCFP (pSB4C5) <b>1</b> miniprep product<br/>
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- | Lane 5: pLAC_fha1_eCFP (pSB4C5) <b>2</b> miniprep product<br/>
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- | Lane 6: DNA ladder 1kb (biolabs)<br/>
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- | Lane 7: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) <b>1</b> miniprep product<br/>
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- | Lane 8: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) <b>2</b> miniprep product<br/>
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- | Lane 9: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>1</b> miniprep product<br/>
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- | Lane 10: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>2</b> miniprep product<br/>
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- | Lane 11: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>3</b> miniprep product<br/>
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- | Lane 12: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>4</b> miniprep product<br/>
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- | Lane 13: DNA ladder 1kb (biolabs)<br/></div>
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- | <br/>
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- | We did some digestions (protocol) on GA miniprep (12/08/03). The digestions were achieved with XbaI during 10 minutes.<br/>
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- | <br/>
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- | To separate the digestion products, we prepared two 1.8% TAE agarose gel.<br/>
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- | Migration conditions = 100V during 30 min.<br/>
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- | In order to reveal the DNA fragments, we used EtBr.<br/>
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- | <br/>
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- | <center><img src="https://static.igem.org/mediawiki/2012/c/cd/120806_dig2.jpg" alt="photo_gel_28"/></center>
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- | <div class="legend"><u>Migration result for a 1.8% TAE agarose gel</u><br/>
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- | <i>(the DNA ladder scale is in kb)</i><br/>
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- | Lane 1: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) <b>1</b> digestion product<br/>
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- | Lane 2: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) <b>1</b> miniprep product<br/>
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- | Lane 3: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) <b>2</b> digestion product<br/>
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- | Lane 4: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) <b>2</b> miniprep product<br/>
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- | Lane 5: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>1</b> digestion product<br/>
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- | Lane 6: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>1</b> miniprep product<br/>
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- | Lane 7: DNA ladder 1kb (biolabs)<br/>
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- | Lane 8: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>2</b> digestion product<br/>
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- | Lane 9: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>2</b> miniprep product<br/>
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- | Lane 10: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>3</b> digestion product<br/>
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- | Lane 11: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>3</b> miniprep product<br/>
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- | Lane 12: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>4</b> digestion product<br/>
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- | Lane 13: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>4</b> miniprep product<br/></div>
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- | <br/>
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- | <center><img src="https://static.igem.org/mediawiki/2012/3/3e/120806.jpg" alt="photo_gel_29"/></center>
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- | <div class="legend"><u>Migration result for a 1.8% TAE agarose gel</u><br/>
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- | <i>(the DNA ladder scale is in kb)</i><br/>
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- | Lane 1: DNA ladder 1kb (biolabs)<br/>
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- | Lane 2: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>1</b> digestion product<br/>
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- | Lane 3: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>1</b> miniprep product<br/>
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- | Lane 4: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>2</b> digestion product<br/>
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- | Lane 5: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>2</b> miniprep product<br/>
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- | Lane 6: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>3</b> digestion product<br/>
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- | Lane 7: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>3</b> miniprep product<br/>
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- | Lane 8:DNA ladder 80pb-10kb (fermentas)<br/>
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- | </div>
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- | <br/>
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- | </section>
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- | </body>
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- | </html>
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- | {{:Team:Grenoble/script}}
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- | {{:Team:Grenoble/menu}}
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