Team:Grenoble/Biology/Notebook/July/week 32

From 2012.igem.org

(Difference between revisions)
Line 22: Line 22:
We wanted to check if the Gibson Assemblies (12/08/01) worked well.<br/>
We wanted to check if the Gibson Assemblies (12/08/01) worked well.<br/>
<br/>
<br/>
-
To separate the GA miniprep (12/08/03) products, we prepared a 1.8% TAE agarose gel.<br/>
+
To separate (<a href="http://2012.igem.org/Team:Grenoble/Biology/Protocols/Gel">protocol</a>) the GA miniprep (12/08/03) products, we prepared a 1.8% TAE agarose gel.<br/>
Migration conditions = 100V during 30 min.<br/>
Migration conditions = 100V during 30 min.<br/>
-
In order to reveal the DNA fragments, we used EtBr.<br/>
+
In order to reveal the DNA fragments, we used <a href="http://2012.igem.org/Team:Grenoble/Biology/Protocols/EtBr">EtBr</a>.<br/>
<br/>
<br/>
<center><img src="http://2012.igem.org/wiki/images/4/44/120806_pcr_miniprep_2.jpg" alt="photo_gel_27"/></center>
<center><img src="http://2012.igem.org/wiki/images/4/44/120806_pcr_miniprep_2.jpg" alt="photo_gel_27"/></center>
Line 44: Line 44:
<br/>
<br/>
<br/>
<br/>
-
We did some digestions (protocol) on GA miniprep (12/08/03). The digestions were achieved with XbaI during 10 minutes.<br/>
+
We did some digestions (<a href="http://2012.igem.org/Team:Grenoble/Biology/Protocols/Restriction">protocol</a>) on GA miniprep (12/08/03). The digestions were achieved with XbaI during 10 minutes.<br/>
<br/>
<br/>
-
To separate the digestion products, we prepared two 1.8% TAE agarose gels.<br/>
+
To separate (<a href="http://2012.igem.org/Team:Grenoble/Biology/Protocols/Gel">protocol</a>) the digestion products, we prepared two 1.8% TAE agarose gels.<br/>
Migration conditions = 100V during 30 min.<br/>
Migration conditions = 100V during 30 min.<br/>
-
In order to reveal the DNA fragments, we used EtBr.<br/>
+
In order to reveal the DNA fragments, we used <a href="http://2012.igem.org/Team:Grenoble/Biology/Protocols/EtBr">EtBr</a>.<br/>
<br/>
<br/>
<center><img src="http://2012.igem.org/wiki/images/c/cd/120806_dig2.jpg" alt="photo_gel_28"/></center>
<center><img src="http://2012.igem.org/wiki/images/c/cd/120806_dig2.jpg" alt="photo_gel_28"/></center>
-
<div class="legend"><u>Migration result for a 1.8% TAE agarose gel</u><br/>
+
<div class="legend"><p><center><u>Migration result for a 1.8% TAE agarose gel</u><br/>
-
   <i>(the DNA ladder scale is in kb)</i><br/>
+
   <i>(the DNA ladder scale is in kb)</i></center></p>
-
Lane 1: data not shown pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) <b>1</b> digestion product<br/>
+
<ul><li><b>Lane 1:</b> data not shown pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) <b>1</b> digestion product</li>
-
Lane 2: data not shown pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) <b>1</b> miniprep product<br/>
+
<li><b>Lane 2:</b> data not shown pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) <b>1</b> miniprep product</li>
-
Lane 3: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) <b>2</b> digestion product<br/>
+
<li><b>Lane 3:</b> pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) <b>2</b> digestion product</li>
-
Lane 4: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) <b>2</b> miniprep product<br/>
+
<li><b>Lane 4:</b> pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) <b>2</b> miniprep product</li>
-
Lane 5: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>4</b> digestion product<br/>
+
<li><b>Lane 5:</b> pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>4</b> digestion product</li>
-
Lane 6: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>4</b> miniprep product<br/>
+
<li><b>Lane 6:</b> pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>4</b> miniprep product</li>
-
Lane 7: DNA ladder 1kb (biolabs)<br/>
+
<li><b>Lane 7:</b> DNA ladder 1kb (biolabs)</li>
-
Lane 8: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>3</b> digestion product<br/>
+
<li><b>Lane 8:</b> pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>3</b> digestion product</li>
-
Lane 9: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>3</b> miniprep product<br/>
+
<li><b>Lane 9:</b> pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>3</b> miniprep product</li>
-
Lane 10: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>1</b> digestion product<br/>
+
<li><b>Lane 10:</b> pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>1</b> digestion product</li>
-
Lane 11: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>1</b> miniprep product<br/>
+
<li><b>Lane 11:</b> pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>1</b> miniprep product</li>
-
Lane 12: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>2</b> digestion product<br/>
+
<li><b>Lane 12:</b> pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>2</b> digestion product</li>
-
Lane 13: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>2</b> miniprep product<br/></div>
+
<li><b>Lane 13:</b> pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>2</b> miniprep product</li></ul></div>
<br/>
<br/>
Line 71: Line 71:
<br/>
<br/>
<center><img src="http://2012.igem.org/wiki/images/3/3e/120806.jpg" alt="photo_gel_29"/></center>
<center><img src="http://2012.igem.org/wiki/images/3/3e/120806.jpg" alt="photo_gel_29"/></center>
-
<div class="legend"><u>Migration result for a 1.8% TAE agarose gel</u><br/>
+
<div class="legend"><p><center><u>Migration result for a 1.8% TAE agarose gel</u><br/>
-
   <i>(the DNA ladder scale is in kb)</i><br/>
+
   <i>(the DNA ladder scale is in kb)</i></center></p>
-
Lane 1: DNA ladder 1kb (biolabs)<br/>
+
<ul><li><b>Lane 1: DNA ladder 1kb (biolabs)<br/>
-
Lane 2: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>4</b> digestion product<br/>
+
<li><b>Lane 2:</b> pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>4</b> digestion product</li>
-
Lane 3: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>4</b> miniprep product<br/>
+
<li><b>Lane 3:</b> pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>4</b> miniprep product</li>
-
Lane 4: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>1</b> digestion product<br/>
+
<li><b>Lane 4:</b> pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>1</b> digestion product</li>
-
Lane 5: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>1</b> miniprep product<br/>
+
<li><b>Lane 5:</b> pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>1</b> miniprep product</li>
-
Lane 6: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>2</b> digestion product<br/>
+
<li><b>Lane 6:</b> pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>2</b> digestion product</li>
-
Lane 7: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>2</b> miniprep product<br/>
+
<li><b>Lane 7:</b> pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) <b>2</b> miniprep product</li>
-
Lane 8:DNA ladder 80pb-10kb (fermentas)<br/>
+
<li><b>Lane 8:</b> DNA ladder 80pb-10kb (fermentas)</li>
 +
</ul>
</div>
</div>
</section>
</section>

Revision as of 15:45, 25 September 2012

iGEM Grenoble 2012

Project

August

Week 31Week 32Week 33Week 34Week 35

Week 32: August 06th to 12th

Goal of the week:

Monday, August 06th:

We wanted to check if the Gibson Assemblies (12/08/01) worked well.

To separate (protocol) the GA miniprep (12/08/03) products, we prepared a 1.8% TAE agarose gel.
Migration conditions = 100V during 30 min.
In order to reveal the DNA fragments, we used EtBr.

photo_gel_27

Migration result for a 1.8% TAE agarose gel
(the DNA ladder scale is in kb)

  • Lane 1: pLAC_rsmY (pSB1A3) 3 miniprep product
  • Lane 2: pLAC_rsmY (pSB1A3) 2 miniprep product
  • Lane 3: pLAC_rsmY (pSB1A3) 1 miniprep product
  • Lane 4: pLAC_fha1_eCFP (pSB4C5) 2 miniprep product
  • Lane 5: pLAC_fha1_eCFP (pSB4C5) 1 miniprep product
  • Lane 6: DNA ladder 1kb (biolabs)
  • Lane 7: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 1 miniprep product
  • Lane 8: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 2 miniprep product
  • Lane 9: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 4 miniprep product
  • Lane 10: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 3 miniprep product
  • Lane 11: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 1 miniprep product
  • Lane 12: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 2 miniprep product
  • Lane 13: DNA ladder 1kb (biolabs)


We did some digestions (protocol) on GA miniprep (12/08/03). The digestions were achieved with XbaI during 10 minutes.

To separate (protocol) the digestion products, we prepared two 1.8% TAE agarose gels.
Migration conditions = 100V during 30 min.
In order to reveal the DNA fragments, we used EtBr.

photo_gel_28

Migration result for a 1.8% TAE agarose gel
(the DNA ladder scale is in kb)

  • Lane 1: data not shown pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 1 digestion product
  • Lane 2: data not shown pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 1 miniprep product
  • Lane 3: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 2 digestion product
  • Lane 4: pAra/Bad_RBS_GFP_RBS_Cya (pSB3C5) 2 miniprep product
  • Lane 5: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 4 digestion product
  • Lane 6: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 4 miniprep product
  • Lane 7: DNA ladder 1kb (biolabs)
  • Lane 8: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 3 digestion product
  • Lane 9: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 3 miniprep product
  • Lane 10: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 1 digestion product
  • Lane 11: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 1 miniprep product
  • Lane 12: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 2 digestion product
  • Lane 13: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 2 miniprep product



photo_gel_29

Migration result for a 1.8% TAE agarose gel
(the DNA ladder scale is in kb)

  • Lane 1: DNA ladder 1kb (biolabs)
  • Lane 2: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 4 digestion product
  • Lane 3: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 4 miniprep product
  • Lane 4: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 1 digestion product
  • Lane 5: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 1 miniprep product
  • Lane 6: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 2 digestion product
  • Lane 7: pAra/Bad_RBS_GFP_RBS_Cya (pSB4C5) 2 miniprep product
  • Lane 8: DNA ladder 80pb-10kb (fermentas)