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Team:Grenoble/Biology/Notebook/July/week 30
From 2012.igem.org
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In order to reveal the DNA fragments, we used EtBr.<br/> | In order to reveal the DNA fragments, we used EtBr.<br/> | ||
<br/> | <br/> | ||
| - | + | <center><img src="https://static.igem.org/mediawiki/2012/c/c1/120723.jpg" alt="photo_gel_16"/></center> | |
| - | + | <div class="legend"><u>Migration result for a 1.3% TAE agarose gel</u><br/> | |
| + | <i>(the DNA ladder scale is in kb)</i><br/> | ||
| + | Lane 1: DNA ladder 80pb-10kb (fermentas)<br/> | ||
| + | Lane 2: pSB4K5 PCR product (DMSO)<br/> | ||
| + | Lane 3: fha1 PCR product (DMSO)<br/> | ||
| + | Lane 4: fha1 PCR product<br/> | ||
| + | Lane 5: pSB4K5 PCR product<br/> | ||
| + | Lane 6: pAra/Bad_RBS_GFP PCR product (DMSO)<br/> | ||
| + | Lane 7: pAra/Bad_RBS_GFP PCR product (DMSO)<br/> | ||
| + | Lane 8: RBS_Cya PCR product (DMSO)<br/> | ||
| + | Lane 9: RBS_Cya PCR product<br/> | ||
| + | Lane 10: pAra/Bad_RBS_GFP PCR product<br/> | ||
| + | Lane 11: pAra/Bad_RBS_GFP PCR product<br/> | ||
| + | Lane 12: DNA ladder 80bp-10kb (fermentas)<br/></div> | ||
| + | <br/> | ||
</section> | </section> | ||
Revision as of 15:34, 7 August 2012
July
week 27 week 28 week 29 week 30Week 30: July 23rd to 29th
Goal of the week:
Test the Gibson Assembly and the transformation protocols, recover and amplify some biobricks involved in our genetic networks :- pAra/Bad_RBS_GFP (1300bp)
- RBS_Cya (2600bp)
- fha (80bp)
- eCFP (800bp)
- pSB4K5 (2400bp)
Monday, July 23rd:
We did PCRs with HF Phusion enzyme (protocol) on miniprep in order to amplify pAra/Bad_RBS_GFP, and on colony to amplify RBS_Cya. We did the PCR both with and without DMSO.To separate the PCR products (12/07/23 and 12/07/20), we prepared a 1.8% TAE agarose gel.
Migration conditions = 100V during 30 min.
In order to reveal the DNA fragments, we used EtBr.
Migration result for a 1.3% TAE agarose gel
(the DNA ladder scale is in kb)
Lane 1: DNA ladder 80pb-10kb (fermentas)
Lane 2: pSB4K5 PCR product (DMSO)
Lane 3: fha1 PCR product (DMSO)
Lane 4: fha1 PCR product
Lane 5: pSB4K5 PCR product
Lane 6: pAra/Bad_RBS_GFP PCR product (DMSO)
Lane 7: pAra/Bad_RBS_GFP PCR product (DMSO)
Lane 8: RBS_Cya PCR product (DMSO)
Lane 9: RBS_Cya PCR product
Lane 10: pAra/Bad_RBS_GFP PCR product
Lane 11: pAra/Bad_RBS_GFP PCR product
Lane 12: DNA ladder 80bp-10kb (fermentas)
(the DNA ladder scale is in kb)
Lane 1: DNA ladder 80pb-10kb (fermentas)
Lane 2: pSB4K5 PCR product (DMSO)
Lane 3: fha1 PCR product (DMSO)
Lane 4: fha1 PCR product
Lane 5: pSB4K5 PCR product
Lane 6: pAra/Bad_RBS_GFP PCR product (DMSO)
Lane 7: pAra/Bad_RBS_GFP PCR product (DMSO)
Lane 8: RBS_Cya PCR product (DMSO)
Lane 9: RBS_Cya PCR product
Lane 10: pAra/Bad_RBS_GFP PCR product
Lane 11: pAra/Bad_RBS_GFP PCR product
Lane 12: DNA ladder 80bp-10kb (fermentas)
