http://2012.igem.org/wiki/index.php?title=Team:Grenoble/Biology/Notebook/July&feed=atom&action=history
Team:Grenoble/Biology/Notebook/July - Revision history
2024-03-29T14:09:59Z
Revision history for this page on the wiki
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http://2012.igem.org/wiki/index.php?title=Team:Grenoble/Biology/Notebook/July&diff=173881&oldid=prev
Adeline millet at 15:31, 25 September 2012
2012-09-25T15:31:02Z
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<td colspan='2' style="background-color: white; color:black;">Revision as of 15:31, 25 September 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>BBa_E0422: eCFP</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>BBa_E0422: eCFP</li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>pSB3C5 plasmid</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>pSB3C5 plasmid</li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li><del class="diffchange diffchange-inline">psB4K5 </del>plasmid</li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li><ins class="diffchange diffchange-inline">pSB4K5 </ins>plasmid</li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></section></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></section></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><section></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><section></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Thursday, July 5<span class="exposant">th</span>:</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Thursday, July 5<span class="exposant">th</span>:</h2></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The transformations (12/07/04) <del class="diffchange diffchange-inline">show </del>no growth on LB Agar plate + antibiotics.<br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The transformations (12/07/04) <ins class="diffchange diffchange-inline">showed </ins>no growth on LB Agar plate + antibiotics.<br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In order to separate (<a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Gel">protocol</a>) the PCR products (12/07/04), we prepared two gels:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In order to separate (<a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Gel">protocol</a>) the PCR products (12/07/04), we prepared two gels:</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>2<span class="exposant">nd</span> group: PCR with GoTaq (<a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/PCR_1">protocol</a>) and Colorless Buffer 5X (+ loading dye for the migration)</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>2<span class="exposant">nd</span> group: PCR with GoTaq (<a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/PCR_1">protocol</a>) and Colorless Buffer 5X (+ loading dye for the migration)</li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br/>For each group, there were <del class="diffchange diffchange-inline">fivz </del>samples (different concentration of template DNA):</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br/>For each group, there were <ins class="diffchange diffchange-inline">five </ins>samples (different concentration of template DNA):</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>1<span class="exposant">st</span> with 1μL of DNA template (= plasmid from the miniprep)</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>1<span class="exposant">st</span> with 1μL of DNA template (= plasmid from the miniprep)</li></div></td></tr>
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Adeline millet
http://2012.igem.org/wiki/index.php?title=Team:Grenoble/Biology/Notebook/July&diff=167253&oldid=prev
Adeline millet at 09:20, 25 September 2012
2012-09-25T09:20:09Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><b>Lane 10:</b> DNA ladder 1kb (biolabs)</li></ul></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><b>Lane 10:</b> DNA ladder 1kb (biolabs)</li></ul></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>There is no visible migration on the second gel (big fragments). We realized a DNA extraction (<a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/DNA_extract">protocol</a>) from the three small fragments <del class="diffchange diffchange-inline">(protocol kit: Nucleospin extract II)</del>:</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>There is no visible migration on the second gel (big fragments). We realized a DNA extraction (<a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/DNA_extract">protocol</a>) from the three small fragments:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>fha1 <span class="code">120705PP_PCR_001 // 120705PP_PCR_002</span></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>fha1 <span class="code">120705PP_PCR_001 // 120705PP_PCR_002</span></li></div></td></tr>
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Adeline millet
http://2012.igem.org/wiki/index.php?title=Team:Grenoble/Biology/Notebook/July&diff=167244&oldid=prev
Adeline millet at 09:19, 25 September 2012
2012-09-25T09:19:26Z
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<td colspan='2' style="background-color: white; color:black;">Revision as of 09:19, 25 September 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><img src="https://static.igem.org/mediawiki/2012/6/67/120705.jpg" alt="photo_gel_2"/></center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><img src="https://static.igem.org/mediawiki/2012/6/67/120705.jpg" alt="photo_gel_2"/></center></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="legend"><p><center><u>Migration results for the 0.8% TAE agarose gel (big fragments)</u><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="legend"><p><center><u>Migration results for the 0.8% TAE agarose gel (big fragments)</u><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><i>(the DNA ladder scale is in kb)</i></center></p<del class="diffchange diffchange-inline">></del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><i>(the DNA ladder scale is in kb)</i></center></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul><li><b>Lane 1:</b> DNA ladder 1kb (biolabs)</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul><li><b>Lane 1:</b> DNA ladder 1kb (biolabs)</li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><b>Lanes 2 and 3:</b> not iGEM PCR products</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><b>Lanes 2 and 3:</b> not iGEM PCR products</li></div></td></tr>
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Adeline millet
http://2012.igem.org/wiki/index.php?title=Team:Grenoble/Biology/Notebook/July&diff=167233&oldid=prev
Adeline millet at 09:18, 25 September 2012
2012-09-25T09:18:42Z
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<td colspan='2' style="background-color: white; color:black;">Revision as of 09:18, 25 September 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><img src="https://static.igem.org/mediawiki/2012/9/90/120705_%282%29.jpg" alt="photo_gel_1"/></center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><img src="https://static.igem.org/mediawiki/2012/9/90/120705_%282%29.jpg" alt="photo_gel_1"/></center></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class="legend"><u>Migration results for the 1.3% TAE agarose gel (small fragments)</u><br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class="legend"<ins class="diffchange diffchange-inline">><p><center</ins>><u>Migration results for the 1.3% TAE agarose gel (small fragments)</u><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><i>(the DNA ladder scale is in kb)</i><<del class="diffchange diffchange-inline">br</del>/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><i>(the DNA ladder scale is in kb)</i></<ins class="diffchange diffchange-inline">center></p</ins>></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Lane 1: DNA ladder 100bp (biolabs)<<del class="diffchange diffchange-inline">br</del>/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><ul><li><b></ins>Lane 1:<ins class="diffchange diffchange-inline"></b> </ins>DNA ladder 100bp (biolabs)</<ins class="diffchange diffchange-inline">li</ins>></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Lanes 2 and 3: not iGEM PCR products<<del class="diffchange diffchange-inline">br</del>/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><li><b></ins>Lanes 2 and 3:<ins class="diffchange diffchange-inline"></b> </ins>not iGEM PCR products</<ins class="diffchange diffchange-inline">li</ins>></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Lanes 4 and 5: RsmA PCR product<<del class="diffchange diffchange-inline">br</del>/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><li><b></ins>Lanes 4 and 5:<ins class="diffchange diffchange-inline"></b> </ins>RsmA PCR product</<ins class="diffchange diffchange-inline">li</ins>></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Lanes 6 and 7: fha1 PCR product<<del class="diffchange diffchange-inline">br</del>/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><li><b></ins>Lanes 6 and 7:<ins class="diffchange diffchange-inline"></b> </ins>fha1 PCR product</<ins class="diffchange diffchange-inline">li</ins>></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Lanes 8 and 9: rsmY PCR product<<del class="diffchange diffchange-inline">br</del>/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><li><b></ins>Lanes 8 and 9:<ins class="diffchange diffchange-inline"></b> </ins>rsmY PCR product</<ins class="diffchange diffchange-inline">li</ins>></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Lane 10: DNA ladder 100bp (biolabs)<<del class="diffchange diffchange-inline">br</del>/></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><li><b></ins>Lane 10:<ins class="diffchange diffchange-inline"></b> </ins>DNA ladder 100bp (biolabs)</<ins class="diffchange diffchange-inline">li></ul</ins>></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><img src="https://static.igem.org/mediawiki/2012/6/67/120705.jpg" alt="photo_gel_2"/></center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><img src="https://static.igem.org/mediawiki/2012/6/67/120705.jpg" alt="photo_gel_2"/></center></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class="legend"><u>Migration results for the 0.8% TAE agarose gel (big fragments)</u><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class="legend"<ins class="diffchange diffchange-inline">><p><center</ins>><u>Migration results for the 0.8% TAE agarose gel (big fragments)</u><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><i>(the DNA ladder scale is in kb)</i><<del class="diffchange diffchange-inline">br</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><i>(the DNA ladder scale is in kb)</i><<ins class="diffchange diffchange-inline">/center></p></ins>></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Lane 1: DNA ladder 1kb (biolabs)<<del class="diffchange diffchange-inline">br</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><ul><li><b></ins>Lane 1:<ins class="diffchange diffchange-inline"></b> </ins>DNA ladder 1kb (biolabs)<<ins class="diffchange diffchange-inline">/li</ins>></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Lanes 2 and 3: not iGEM PCR products<<del class="diffchange diffchange-inline">br</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><li><b></ins>Lanes 2 and 3:<ins class="diffchange diffchange-inline"></b> </ins>not iGEM PCR products<<ins class="diffchange diffchange-inline">/li</ins>></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Lanes 4 and 5: pSB1A3 PCR product<<del class="diffchange diffchange-inline">br</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><li><b></ins>Lanes 4 and 5:<ins class="diffchange diffchange-inline"></b> </ins>pSB1A3 PCR product<<ins class="diffchange diffchange-inline">/li</ins>></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Lanes 6 and 7: RBS_Cya PCR product<<del class="diffchange diffchange-inline">br</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><li><b></ins>Lanes 6 and 7:<ins class="diffchange diffchange-inline"></b> </ins>RBS_Cya PCR product<<ins class="diffchange diffchange-inline">/li</ins>></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Lanes 8 and 9: pAra/Bad_RBS_GFP PCR product<<del class="diffchange diffchange-inline">br</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><li><b></ins>Lanes 8 and 9:<ins class="diffchange diffchange-inline"></b> </ins>pAra/Bad_RBS_GFP PCR product<<ins class="diffchange diffchange-inline">/li</ins>></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Lane 10: DNA ladder 1kb (biolabs)<<del class="diffchange diffchange-inline">br</del>></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><li><b></ins>Lane 10:<ins class="diffchange diffchange-inline"></b> </ins>DNA ladder 1kb (biolabs)<<ins class="diffchange diffchange-inline">/li></ul</ins>></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>There is no visible migration on the second gel (big fragments). We realized a DNA extraction (<a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/DNA_extract">protocol</a>) from the three small fragments (protocol kit: Nucleospin extract II):</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>There is no visible migration on the second gel (big fragments). We realized a DNA extraction (<a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/DNA_extract">protocol</a>) from the three small fragments (protocol kit: Nucleospin extract II):</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><img src="https://static.igem.org/mediawiki/2012/c/c1/120705_%283%29.jpg" alt="photo_gel_3"/></center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><img src="https://static.igem.org/mediawiki/2012/c/c1/120705_%283%29.jpg" alt="photo_gel_3"/></center></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class="legend"><u>Migration results for a 0.8% TAE agarose gel</u><br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class="legend"<ins class="diffchange diffchange-inline">><p><center</ins>><u>Migration results for a 0.8% TAE agarose gel</u><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <i>(the DNA ladder scale is in kb)</i><<del class="diffchange diffchange-inline">br</del>/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <i>(the DNA ladder scale is in kb)</i></<ins class="diffchange diffchange-inline">center></p</ins>></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Lane 1: DNA ladder 1kb (biolabs)<<del class="diffchange diffchange-inline">br</del>/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><ul><li><b></ins>Lane 1:<ins class="diffchange diffchange-inline"></b> </ins>DNA ladder 1kb (biolabs)</<ins class="diffchange diffchange-inline">li</ins>></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Lanes 2 and 3: RBS_Cya PCR product<<del class="diffchange diffchange-inline">br</del>/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><li><b></ins>Lanes 2 and 3:<ins class="diffchange diffchange-inline"></b> </ins>RBS_Cya PCR product</<ins class="diffchange diffchange-inline">li</ins>></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Lanes 4 and 5: not iGEM PCR product<<del class="diffchange diffchange-inline">br</del>/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><li><b></ins>Lanes 4 and 5:<ins class="diffchange diffchange-inline"></b> </ins>not iGEM PCR product</<ins class="diffchange diffchange-inline">li</ins>></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Lanes 6 and 7: not iGEM PCR product<<del class="diffchange diffchange-inline">br</del>/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><li><b></ins>Lanes 6 and 7:<ins class="diffchange diffchange-inline"></b> </ins>not iGEM PCR product</<ins class="diffchange diffchange-inline">li</ins>></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Lanes 8 and 9: pSB1A3 PCR product<<del class="diffchange diffchange-inline">br</del>/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><li><b></ins>Lanes 8 and 9:<ins class="diffchange diffchange-inline"></b> </ins>pSB1A3 PCR product</<ins class="diffchange diffchange-inline">li</ins>></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Lane 10: DNA ladder 1kb (biolabs)<<del class="diffchange diffchange-inline">br</del>/></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><li><b></ins>Lane 10:<ins class="diffchange diffchange-inline"></b> </ins>DNA ladder 1kb (biolabs)</<ins class="diffchange diffchange-inline">li></ul</ins>></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>There is no visible migration on the gel, we only saw primer dimer bands. There was a PCR condition problem.<br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>There is no visible migration on the gel, we only saw primer dimer bands. There was a PCR condition problem.<br/></div></td></tr>
</table>
Adeline millet
http://2012.igem.org/wiki/index.php?title=Team:Grenoble/Biology/Notebook/July&diff=166663&oldid=prev
Adeline millet at 08:33, 25 September 2012
2012-09-25T08:33:50Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 08:33, 25 September 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 106:</td>
<td colspan="2" class="diff-lineno">Line 106:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>To separate the PCR products, we prepared a 0.8% TAE agarose gel.<br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>To separate <ins class="diffchange diffchange-inline">(<a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Gel">protocol</a>) </ins>the PCR products, we prepared a 0.8% TAE agarose gel.<br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Migration conditions = 50V during 1h35.<br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Migration conditions = 50V during 1h35.<br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The DNA fragments were revealed with EtBr.<br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The DNA fragments were revealed with <ins class="diffchange diffchange-inline"><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/</ins>EtBr<ins class="diffchange diffchange-inline">">EtBr</a></ins>.<br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><img src="https://static.igem.org/mediawiki/2012/c/c1/120705_%283%29.jpg" alt="photo_gel_3"/></center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><img src="https://static.igem.org/mediawiki/2012/c/c1/120705_%283%29.jpg" alt="photo_gel_3"/></center></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>As our PCR results were not conclusive, we decided to test the PCR conditions.<br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>As our PCR results were not conclusive, we decided to test the PCR conditions.<br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We did a miniprep (<del class="diffchange diffchange-inline">protocol kit</del>: <del class="diffchange diffchange-inline">Nucleospin plasmid Quick Pure</del>) on BW25113 cya<span class="exposant">-</span> pAra/Bad strain, in order to recover the plasmid with pAra/Bad_RBS_GFP on it.<br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We did a miniprep (<ins class="diffchange diffchange-inline"><a href="https</ins>:<ins class="diffchange diffchange-inline">//2012.igem.org/Team:Grenoble/Biology/Protocols/Miniprep">protocol</a></ins>) on BW25113 cya<span class="exposant">-</span> pAra/Bad strain, in order to recover the plasmid with pAra/Bad_RBS_GFP on it.<br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To test the PCR conditions, we separated the sample in two groups (to check the Green buffer effect) subdivised into five samples according to the amount of template DNA:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To test the PCR conditions, we separated the sample in two groups (to check the Green buffer effect) subdivised into five samples according to the amount of template DNA:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li>1<span class="exposant">st</span> group: PCR with GoTaq (protocol) and Green Buffer 5X</li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li>1<span class="exposant">st</span> group: PCR with GoTaq (<ins class="diffchange diffchange-inline"><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/PCR_1"></ins>protocol<ins class="diffchange diffchange-inline"></a></ins>) and Green Buffer 5X</li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li>2<span class="exposant">nd</span> group: PCR with GoTaq (protocol) and Colorless Buffer 5X (+ loading dye for the migration)</li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li>2<span class="exposant">nd</span> group: PCR with GoTaq (<ins class="diffchange diffchange-inline"><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/PCR_1"></ins>protocol<ins class="diffchange diffchange-inline"></a></ins>) and Colorless Buffer 5X (+ loading dye for the migration)</li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>For each group, there were fivz samples (different concentration of template DNA):</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>For each group, there were fivz samples (different concentration of template DNA):</div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 152:</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>the 1<span class="exposant">st</span>: no digestion</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>the 1<span class="exposant">st</span>: no digestion</li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li>the 2<span class="exposant">nd</span>: one hour digestion (protocol) by a restriction enzyme (=XbaI)</li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li>the 2<span class="exposant">nd</span>: one hour digestion (<ins class="diffchange diffchange-inline"><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Restriction"></ins>protocol<ins class="diffchange diffchange-inline"></a></ins>) by a restriction enzyme (=XbaI)</li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li>the 3<span class="exposant">rd</span>: 10 min digestion (protocol) by a restriction enzyme (=XbaI)</li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li>the 3<span class="exposant">rd</span>: 10 min digestion (<ins class="diffchange diffchange-inline"><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Restriction"></ins>protocol<ins class="diffchange diffchange-inline"></a></ins>) by a restriction enzyme (=XbaI)</li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>For each group, there were five samples with different concentration of DNA:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>For each group, there were five samples with different concentration of DNA:</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>5<span class="exposant">th</span> with 0μL of DNA template</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>5<span class="exposant">th</span> with 0μL of DNA template</li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br/>To separate the PCR products and the digestion products, we prepared a 0.8% TAE agarose gel.<br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br/>To separate <ins class="diffchange diffchange-inline">(<a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Gel">protocol</a>) </ins>the PCR products and the digestion products, we prepared a 0.8% TAE agarose gel.<br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Migration conditions = 50V during 1h35.<br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Migration conditions = 50V during 1h35.<br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The DNA fragments were revealed with EtBr.<br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The DNA fragments were revealed with <ins class="diffchange diffchange-inline"><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/</ins>EtBr<ins class="diffchange diffchange-inline">">EtBr</a></ins>.<br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>There was no visible migration on the gel (data not shown).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>There was no visible migration on the gel (data not shown).</div></td></tr>
</table>
Adeline millet
http://2012.igem.org/wiki/index.php?title=Team:Grenoble/Biology/Notebook/July&diff=166599&oldid=prev
Adeline millet at 08:28, 25 September 2012
2012-09-25T08:28:05Z
<p></p>
<table style="background-color: white; color:black;">
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<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 08:28, 25 September 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 59:</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The transformations (12/07/04) show no growth on LB Agar plate + antibiotics.<br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The transformations (12/07/04) show no growth on LB Agar plate + antibiotics.<br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>In order to separate (<a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Gel"protocol</a>) the PCR products (12/07/04), we prepared two gels:</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>In order to separate (<a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Gel"<ins class="diffchange diffchange-inline">></ins>protocol</a>) the PCR products (12/07/04), we prepared two gels:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>a 1.3% Tris base Acetic acid EDTA (TAE) agarose gel, for the small fragments: RsmA, fha1 and rsmY (length < 1000bp)</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>a 1.3% Tris base Acetic acid EDTA (TAE) agarose gel, for the small fragments: RsmA, fha1 and rsmY (length < 1000bp)</li></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Migration conditions = 100V during 35 min.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Migration conditions = 100V during 35 min.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br/>In order to reveal the fragments, we used <a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/EtBr">Ethidium Bromide (EtBr).<br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br/>In order to reveal the fragments, we used <a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/EtBr">Ethidium Bromide (EtBr)<ins class="diffchange diffchange-inline"></a></ins>.<br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><img src="https://static.igem.org/mediawiki/2012/9/90/120705_%282%29.jpg" alt="photo_gel_1"/></center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><img src="https://static.igem.org/mediawiki/2012/9/90/120705_%282%29.jpg" alt="photo_gel_1"/></center></div></td></tr>
</table>
Adeline millet
http://2012.igem.org/wiki/index.php?title=Team:Grenoble/Biology/Notebook/July&diff=166593&oldid=prev
Adeline millet at 08:27, 25 September 2012
2012-09-25T08:27:11Z
<p></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 08:27, 25 September 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><section></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><section></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Wednesday, July 04<span class="exposant">th</span>:</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Wednesday, July 04<span class="exposant">th</span>:</h2></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We did PCRs on colony with a GoTaq enzyme (protocol) to amplify:</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We did PCRs on colony with a GoTaq enzyme (<ins class="diffchange diffchange-inline"><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/PCR_1"></ins>protocol<ins class="diffchange diffchange-inline"></a></ins>) to amplify:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>fha1, RsmA, rsmY and pSB1A3 from iGEM Grenoble 2011 glycerol stock.</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>fha1, RsmA, rsmY and pSB1A3 from iGEM Grenoble 2011 glycerol stock.</li></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 47:</td>
<td colspan="2" class="diff-lineno">Line 47:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Using iGEM 2012 biobricks we transformed BW25113 WT cells (protocol). We obtained four transformed strains with four different biobricks:</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Using iGEM 2012 biobricks we transformed BW25113 WT cells (<ins class="diffchange diffchange-inline"><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Transformation_1"></ins>protocol<ins class="diffchange diffchange-inline"></a></ins>). We obtained four transformed strains with four different biobricks:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>BBa_I13601: pLAC_RBS</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>BBa_I13601: pLAC_RBS</li></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 59:</td>
<td colspan="2" class="diff-lineno">Line 59:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The transformations (12/07/04) show no growth on LB Agar plate + antibiotics.<br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The transformations (12/07/04) show no growth on LB Agar plate + antibiotics.<br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>In order to separate the PCR products (12/07/04), we prepared two gels:</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>In order to separate <ins class="diffchange diffchange-inline">(<a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/Gel"protocol</a>) </ins>the PCR products (12/07/04), we prepared two gels:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>a 1.3% Tris base Acetic acid EDTA (TAE) agarose gel, for the small fragments: RsmA, fha1 and rsmY (length < 1000bp)</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>a 1.3% Tris base Acetic acid EDTA (TAE) agarose gel, for the small fragments: RsmA, fha1 and rsmY (length < 1000bp)</li></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 65:</td>
<td colspan="2" class="diff-lineno">Line 65:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Migration conditions = 100V during 35 min.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Migration conditions = 100V during 35 min.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br/>In order to reveal the fragments, we used Ethidium Bromide (EtBr).<br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br/>In order to reveal the fragments, we used <ins class="diffchange diffchange-inline"><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/EtBr"></ins>Ethidium Bromide (EtBr).<br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><img src="https://static.igem.org/mediawiki/2012/9/90/120705_%282%29.jpg" alt="photo_gel_1"/></center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><img src="https://static.igem.org/mediawiki/2012/9/90/120705_%282%29.jpg" alt="photo_gel_1"/></center></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 87:</td>
<td colspan="2" class="diff-lineno">Line 87:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Lane 10: DNA ladder 1kb (biolabs)<br></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Lane 10: DNA ladder 1kb (biolabs)<br></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>There is no visible migration on the second gel (big fragments). We realized a DNA extraction from the three small fragments (protocol kit: Nucleospin extract II):</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>There is no visible migration on the second gel (big fragments). We realized a DNA extraction <ins class="diffchange diffchange-inline">(<a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/DNA_extract">protocol</a>) </ins>from the three small fragments (protocol kit: Nucleospin extract II):</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>fha1 <span class="code">120705PP_PCR_001 // 120705PP_PCR_002</span></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>fha1 <span class="code">120705PP_PCR_001 // 120705PP_PCR_002</span></li></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 100:</td>
<td colspan="2" class="diff-lineno">Line 100:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We did colony PCR with a GoTaq enzyme (protocol) to amplify:</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We did colony PCR with a GoTaq enzyme (<ins class="diffchange diffchange-inline"><a href="https://2012.igem.org/Team:Grenoble/Biology/Protocols/PCR_1"></ins>protocol<ins class="diffchange diffchange-inline"></a></ins>) to amplify:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>RBS_Cya from BW25113 WT strain</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>RBS_Cya from BW25113 WT strain</li></div></td></tr>
</table>
Adeline millet
http://2012.igem.org/wiki/index.php?title=Team:Grenoble/Biology/Notebook/July&diff=75568&oldid=prev
Adeline millet at 13:52, 20 August 2012
2012-08-20T13:52:07Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 13:52, 20 August 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>There was no visible migration on the gel (data not shown).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>There was no visible migration on the gel (data not shown).</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></section></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></section></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><br/></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><section></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><section></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Conclusion of the week:</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Conclusion of the week:</h2></div></td></tr>
</table>
Adeline millet
http://2012.igem.org/wiki/index.php?title=Team:Grenoble/Biology/Notebook/July&diff=75566&oldid=prev
Adeline millet at 13:51, 20 August 2012
2012-08-20T13:51:44Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 13:51, 20 August 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 11:</td>
<td colspan="2" class="diff-lineno">Line 11:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="https://2012.igem.org/Team:Grenoble/Biology/Notebook/July/week_30">Week 30</a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="https://2012.igem.org/Team:Grenoble/Biology/Notebook/July/week_30">Week 30</a></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></section></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></section></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><br/></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><section></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><section></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 31:</td>
<td colspan="2" class="diff-lineno">Line 30:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></section></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></section></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><br/></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><section></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><section></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2> Tuesday, July 03<span class="exposant">rd</span>:</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2> Tuesday, July 03<span class="exposant">rd</span>:</h2></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 40:</td>
<td colspan="2" class="diff-lineno">Line 38:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></section></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></section></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><br/></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><section></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><section></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Wednesday, July 04<span class="exposant">th</span>:</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Wednesday, July 04<span class="exposant">th</span>:</h2></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 58:</td>
<td colspan="2" class="diff-lineno">Line 55:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></section></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></section></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><br/></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><section></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><section></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Thursday, July 5<span class="exposant">th</span>:</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Thursday, July 5<span class="exposant">th</span>:</h2></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 132:</td>
<td colspan="2" class="diff-lineno">Line 128:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></section></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></section></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><br/></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><section></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><section></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Friday, July 6<span class="exposant">th</span>:</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Friday, July 6<span class="exposant">th</span>:</h2></div></td></tr>
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<td colspan="2" class="diff-lineno">Line 174:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>As we had no conclusive results, we have decided to change the protocols.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>As we had no conclusive results, we have decided to change the protocols.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></section></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></section></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><br/></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><br/></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></body></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></body></div></td></tr>
</table>
Adeline millet
http://2012.igem.org/wiki/index.php?title=Team:Grenoble/Biology/Notebook/July&diff=75458&oldid=prev
Adeline millet at 12:46, 20 August 2012
2012-08-20T12:46:31Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 12:46, 20 August 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><section></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><section></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1> Week 27: July 02<span class="exposant">nd</span> to 08<span class="exposant">th</span> </h1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1> Week 27: July 02<span class="exposant">nd</span> to 08<span class="exposant">th</span> </h1></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2> Goal of the week: </h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2> Goal of the week: </h2></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We wanted to test the PCR conditions, recover and amplify the biobricks involved in our genetic networks:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We wanted to test the PCR conditions, recover and amplify the biobricks involved in our genetic networks:</div></td></tr>
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Adeline millet