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Team:Grenoble/Biology/Notebook/August
From 2012.igem.org
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<h2> Tuesday, July 31<span class="exposant">th</span>:</h2> | <h2> Tuesday, July 31<span class="exposant">th</span>:</h2> | ||
| + | We did a miniprep (protocol kit: Nucleospin plasmid Quick Pure) on the transformed strain with pSB4C5, on which we wanted to recover pSB4C5.<br/> | ||
| + | <br/> | ||
| + | We did some colony PCRs with HF Phusion enzyme (protocol) in order to amplify pSB4C5 (on 12/07/25 colony).<br/> | ||
| + | <br/> | ||
| + | To separate the PCR products, we prepared a 1.8% TAE agarose gel.<br/> | ||
| + | Migration conditions = 100V during 30 min.<br/> | ||
| + | In order to reveal the DNA fragments, we used EtBr.<br/> | ||
| + | <br/> | ||
| + | <center><img src="https://static.igem.org/mediawiki/2012/c/c1/120723.jpg" alt="photo_gel_22"/></center> | ||
Revision as of 08:30, 9 August 2012
August
week 31 week 32 week 33 week 34 week 35Week 31: July 30th to August 05th
Goal of the week:
We wanted to recover and amplify some biobricks involved in our genetic networks :- pSB4C5 (2400bp)
- pompC (100bp)
- mcherry (900bp)
- eCFP (800bp)
- GFP (100bp)
Tuesday, July 31th:
We did a miniprep (protocol kit: Nucleospin plasmid Quick Pure) on the transformed strain with pSB4C5, on which we wanted to recover pSB4C5.We did some colony PCRs with HF Phusion enzyme (protocol) in order to amplify pSB4C5 (on 12/07/25 colony).
To separate the PCR products, we prepared a 1.8% TAE agarose gel.
Migration conditions = 100V during 30 min.
In order to reveal the DNA fragments, we used EtBr.
