Team:Grenoble/Biology/Notebook

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iGEM Grenoble 2012

Project

Notebook

Greetings iGEMers,
on these pages you will be able to read our lab book (for your greatest pleasure of course)(probably the next world's bestseller !!!).
In order to make the notebook as easy to read as possible; we decided to divide it into groups (Months). Each of them are divided into subgroups (Weeks).
Each group will have a short summary, so you don't have to get through the whole notebook in order to find the good stuff!

June

Week 23 : Choice and description of the project.
Week 24 : RsmA-rsmY system (amplifier) design and research on a possible detection module.
Week 25 : Second amplifier design (cAMP-CRP system)
Week 26 : Detection module description, RsmA-rsmY system and cAMP-CRP network.

July

Week 27 : Test PCR conditions, recovering and amplifying the biobricks involved in our genetic networks --> no significant results.
Week 28 : Recovery and amplification of the different biobricks --> Amplcification of pLAC (fha1) // pLAC (rsmY) // pLAC_RBS // RsmA // rsmY // pSB1A3 // pSB3C5 (Cya) // pSB3C5 (RsmA).
Week 29 : Gibson assembly, transformation, amplification and recovery of biobricks --> succesful Gibson Assembly: pLAC_RBS_RsmA on pSB3C5
Week 30 : Amplification of pAra/Bad_RBS_GFP // RBS_Cya // fha // pSB4C5

August

Week 31 :
Week 32 :
Week 33 :
Week 34 :
Week 35 :

September

Week 36 :
Week 37 :
Week 38 :
Week 39 :

October

Week 40 :
Week 41 :
Week 42 :
Week 43 :