Team:Grenoble/Biology/Network

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iGEM Grenoble 2012

Project

Network details

Our system is divided in two modules:
  • signaling module
  • amplification module

Signaling module

The signaling module allows our bacterial strain to integrate the input signal = the pathogene presence.
Stapylococcus aureus secrete a protease nom de la protéase which cut a specific amino-acids sequence. This specific sequence can be used as a linker between a membrane protein and a dipeptide.
Once S. aureus is present, the linker is cut by the protease and the dipeptide is released.

The dipeptide bind to his receptor which is an engineered receptor:
  • the extracellular part is the extracellular part of Tap, a dipeptide receptor involved in the chemotaxism
  • the intracellular part is the intracellular part of EnvZ, a kinase involved in the osmoregulation

Once the dipeptide is bound, the EnvZ part allows the phosphorylation of OmpR, a transcriptional activator.

Amplification module

The amplification module allows our bacterial strain to amplify the input signal and to produce an output signal = fluorescence.

Once OmpR is phosphorylated, it allows the production of adenyl cyclase by activating the OmpC promoter.
Adenyl cyclase is an enzyme which catalyse the conversion of ATP (Adenosine Tri-Phosphate) to cAMP (cyclic Adenosine Mono-Phosphate)