Team:Grenoble/Biology/Network

From 2012.igem.org

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Once the dipeptide binds the Tap part <a href="https://2012.igem.org/Team:Grenoble/Biology/Network#30">[7]</a>, the intracellular EnvZ part allows the phosphorylation of OmpR <a href="https://2012.igem.org/Team:Grenoble/Biology/Network#30">[8]</a> <a href="https://2012.igem.org/Team:Grenoble/Biology/Network#30">[9]</a>, which is a constitutively produced transcriptional activator.<br/>
Once the dipeptide binds the Tap part <a href="https://2012.igem.org/Team:Grenoble/Biology/Network#30">[7]</a>, the intracellular EnvZ part allows the phosphorylation of OmpR <a href="https://2012.igem.org/Team:Grenoble/Biology/Network#30">[8]</a> <a href="https://2012.igem.org/Team:Grenoble/Biology/Network#30">[9]</a>, which is a constitutively produced transcriptional activator.<br/>
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OmpR phosphorylation's allows the activation of the OmpC promoter <a href="https://2012.igem.org/Team:Grenoble/Biology/Network#30">[10]</a>.
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OmpR phosphorylation's allows the activation of the ompC promoter <a href="https://2012.igem.org/Team:Grenoble/Biology/Network#30">[10]</a>.
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<center><img src="https://static.igem.org/mediawiki/2012/c/c7/AND.png"/></center>
<center><img src="https://static.igem.org/mediawiki/2012/c/c7/AND.png"/></center>
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The binding of cAMP to CRP (C-reactive protein) leads to the production of AraC by activating the pMalT promoter <a href="https://2012.igem.org/Team:Grenoble/Biology/Network#30">[12]</a>.<br/>
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The binding of cAMP to CRP (C-reactive protein) leads to the production of AraC by activating the pmalT promoter <a href="https://2012.igem.org/Team:Grenoble/Biology/Network#30">[12]</a>.<br/>
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In the presence of arabinose, AraC and cAMP-CRP, cooperatively activate the pAraBAD promoter <a href="https://2012.igem.org/Team:Grenoble/Biology/Network#30">[13]</a>, thus forming an "AND" gate. This allows the production of:
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In the presence of arabinose, AraC and cAMP-CRP, cooperatively activate the paraBAD promoter <a href="https://2012.igem.org/Team:Grenoble/Biology/Network#30">[13]</a>, thus forming an "AND" gate. This allows the production of:
<ul><li>Adenyl cyclase which reproduces cAMP, forming thus an amplification loop
<ul><li>Adenyl cyclase which reproduces cAMP, forming thus an amplification loop
<li>GFP (Green Fluorescent Protein) = our output signal
<li>GFP (Green Fluorescent Protein) = our output signal

Revision as of 15:17, 25 September 2012

iGEM Grenoble 2012

Project

Network details

Our system is divided in two modules:
  • a signaling module
  • an amplification module

The signaling module

The signaling module allows our bacteria strain to integrate the input signal = the pathogene presence.

This is a modelized module.


The idea behind this module comes from the iGEM London Imperial College 2010 Team's work on Parasight [1].

Staphylococcus aureus secretes the exfoliative toxin B [2] which cleaves a specific amino-acids sequence (Desmoglein 1). This specific sequence can be used as a linker between a membrane protein and a dipeptide.
Once S. aureus is present, the linker is cut by the toxin and the dipeptide is released.

The dipeptide binds its receptor which was engineered [3] [4] by the team:
  • the extracellular part of Tap [5] is a dipeptide receptor involved in the chemotaxism
  • the intracellular part of EnvZ [6] is a histidine kinase involved in the osmoregulation

Once the dipeptide binds the Tap part [7], the intracellular EnvZ part allows the phosphorylation of OmpR [8] [9], which is a constitutively produced transcriptional activator.

OmpR phosphorylation's allows the activation of the ompC promoter [10].

Amplification module

The amplification module allows our bacteria to amplify the input signal and to produce an output signal = fluorescence.

This is also one of our module of modeling.

Internal amplification


The activation of the OmpC promoter allows the production of Adenyl cyclase [11]. Adenyl cyclase catalyses the conversion of ATP (Adenosine Tri-Phosphate) into cAMP (cyclic Adenosine Mono-Phosphate).


The binding of cAMP to CRP (C-reactive protein) leads to the production of AraC by activating the pmalT promoter [12].
In the presence of arabinose, AraC and cAMP-CRP, cooperatively activate the paraBAD promoter [13], thus forming an "AND" gate. This allows the production of:
  • Adenyl cyclase which reproduces cAMP, forming thus an amplification loop
  • GFP (Green Fluorescent Protein) = our output signal

External amplification

When a bacterium detects S. aureus, it produces a several molecules of GFP and evenmore cAMP. cAMP diffuses through the membrane and activates the amplification loop in all the neighbouring bacteria [14], which triggers the production of GFP and cAMP.
This leads to an entire population which produces GFP where only a bacterium detected the pathogen in the first place:


References