Team:Georgia Tech

From 2012.igem.org

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==[https://2012.igem.org/Team:Georgia_Tech/Project Project] Abstract==
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Our goal is to engineer a novel biosensor with a faster readout than is currently available. Many bacteria produce, secrete, and respond to chemicals called autoinducers to monitor population density and to synchronize gene expression, a process called quorum sensing. In quorum sensing based biosensors, detection of autoinducer activates transcription of a reporter gene, which must then be translated and accumulate to detectable levels, which can take two to four hours. In our system, we will use TraR, a protein used in the quorum sensing response of Agrobacterium tumefaciens, which dimerizes only in the presence of its autoinducer. We have successfully fused traR to sequence for two separate complementary fragments of GFP. Upon addition of autoinducer, we predict that already accumulated TraR-GFP fragment monomers will dimerize, allowing the GFP fragments to interact and fluoresce. This new approach may drastically reduce the time necessary for future biosensors to produce detectable output.
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==A new approach to [https://2012.igem.org/Team:Georgia_Tech/HumanPractices Human Practices]==
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Here we present an outline of our workshop for the benefit of other collegiate iGEM teams reaching out to high school iGEM teams. We believe that formal collaborations between college and high school represent an innovative new approach to human practices with benefits for both the high school and collegiate teams. This was an effective way for us to engender excitement about both synthetic biology in high school level students as well as for our college students to demonstrate further mastery of the field of synthetic biology in general and their research topic specifically by creating presentations and activities designed for the high school level. Visit our [https://2012.igem.org/Team:Georgia_Tech/HumanPractices Human Practices] page to see our idea in action
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==Thanks for all your help!==
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We received lots of help from researchers with no vested interest in our success. We especially want to thank Dr. Clay Fuqua from University of Indiana and Dr. Lynne Regan from Yale University. Check out our [https://2012.igem.org/Team:Georgia_Tech/Attributions Attributions] page for more information.
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|You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
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''Currently, scientific endeavors are being pursued to engineer a synthetic quorum sensing system in E. Coli to express green fluorescent protein (GFP) in response to exogenous quorum sensing molecules. However, certain limitation exists as regards to the time required for the expression of GFP fluorescence. Anywhere from thirty minutes to two hours is necessary from the addition of the autoinducer until sufficient GFP can be transcribed, translated, accumulated, and detected, which for some synthetic biology applications may be unacceptable.  
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To this end, the goal of our experiment is to develop an alternative method to quickly visualize the response of E. coli to exogenous autoinducer by expressing the already present fusion proteins of GFP to the autoinducer receptor. Specifically, the C-terminus end of the GFP protein would be fused to one copy of the receptor gene which will be TraR from Agrobacterium tumefaciens, with the N-terminus end fused to a second copy of the same receptor. The GFP/receptor domain will get continuously transcribed and translated in the bacteria but it will not be able to fluoresce as the domains do not constitute a functional GFP protein. This will be brought about by the dimerization of the TraR receptor in the presence of autoinducers. This alternative method of inducing GFP expression is hypothesized to be efficient and quicker than the methods currently used as it bypasses the transcription and translation machinery of the cell while expressing the GFP.
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==Judges==
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|[[Image:Georgia_Tech_team.png|right|frame|Your team picture]]
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|align="center"|[[Team:Georgia_Tech | Team Georgia_Tech]]
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<!--- The Mission, Experiments --->
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Here is our [https://igem.org/2012_Judging_Form?id=916 judging form] and our [https://igem.org/Team.cgi?year=2012&team_name=Georgia_Tech official team profile].
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We characterized a part from the registry and posted our data on the [http://partsregistry.org/Part:BBa_K091134:Experience#Georgia_Tech_2012_iGEM_Team_Review part's experience page].
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!align="center"|[[Team:Georgia_Tech|Home]]
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!align="center"|[[Team:Georgia_Tech/Team|Team]]
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We were also featured in a local newspaper for our new approach to human practices. We posted the link on the [https://2012.igem.org/IGEM_Publicity#team_specific iGEM publicity page].
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!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=Georgia_Tech Official Team Profile]
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!align="center"|[[Team:Georgia_Tech/Project|Project]]
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Our designed BioBricks can be found on our project page [https://2012.igem.org/Team:Georgia_Tech/Parts here.] We are in the process of making sure that compatible DNA gets submitted to the registry as soon as possible for two of the submitted parts. We are in contact Vinoo at iGEM headquarters who is helping us as we resolve this issue.
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!align="center"|[[Team:Georgia_Tech/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Georgia_Tech/Modeling|Modeling]]
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!align="center"|[[Team:Georgia_Tech/Notebook|Notebook]]
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!align="center"|[[Team:Georgia_Tech/Safety|Safety]]
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!align="center"|[[Team:Georgia_Tech/Attributions|Attributions]]
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Latest revision as of 02:45, 4 October 2012

Igem-logo.png

GeorgiaTech tower.png

Project Abstract

Our goal is to engineer a novel biosensor with a faster readout than is currently available. Many bacteria produce, secrete, and respond to chemicals called autoinducers to monitor population density and to synchronize gene expression, a process called quorum sensing. In quorum sensing based biosensors, detection of autoinducer activates transcription of a reporter gene, which must then be translated and accumulate to detectable levels, which can take two to four hours. In our system, we will use TraR, a protein used in the quorum sensing response of Agrobacterium tumefaciens, which dimerizes only in the presence of its autoinducer. We have successfully fused traR to sequence for two separate complementary fragments of GFP. Upon addition of autoinducer, we predict that already accumulated TraR-GFP fragment monomers will dimerize, allowing the GFP fragments to interact and fluoresce. This new approach may drastically reduce the time necessary for future biosensors to produce detectable output.

A new approach to Human Practices

Here we present an outline of our workshop for the benefit of other collegiate iGEM teams reaching out to high school iGEM teams. We believe that formal collaborations between college and high school represent an innovative new approach to human practices with benefits for both the high school and collegiate teams. This was an effective way for us to engender excitement about both synthetic biology in high school level students as well as for our college students to demonstrate further mastery of the field of synthetic biology in general and their research topic specifically by creating presentations and activities designed for the high school level. Visit our Human Practices page to see our idea in action

Thanks for all your help!

We received lots of help from researchers with no vested interest in our success. We especially want to thank Dr. Clay Fuqua from University of Indiana and Dr. Lynne Regan from Yale University. Check out our Attributions page for more information.

Judges

Here is our judging form and our official team profile.

We characterized a part from the registry and posted our data on the part's experience page.

We were also featured in a local newspaper for our new approach to human practices. We posted the link on the iGEM publicity page.

Our designed BioBricks can be found on our project page here. We are in the process of making sure that compatible DNA gets submitted to the registry as soon as possible for two of the submitted parts. We are in contact Vinoo at iGEM headquarters who is helping us as we resolve this issue.