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Team:Freiburg/Project/Experiments
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=<span style="color:#2244AA"> Gene activation = | =<span style="color:#2244AA"> Gene activation = | ||
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| + | <div align="justify">To show the functionality of our TAL protein as well as the impact of the VP 64 transcription factor fusion protein, we used a TAL-VP64 fusion construct targetting a minimal promotor coupled with the secreated alkaline phosphatase (SEAP). | ||
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| + | The experiment was done with four different transfections, either no plasmid, only the TAL vector, only the SEAP plasmid or a cotransfection of both plasmids. | ||
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| - | + | <a target="_blank"><img style="margin-left:200px;" src="http://imageshack.us/a/img845/4594/exp1design.png" padding:0px; width="300px"; /></a><br> | |
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| - | <a target="_blank"><img style="margin-left:200px;" src="http://imageshack.us/a/ | + | |
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[[#top|Back to top]] | [[#top|Back to top]] | ||
Revision as of 22:47, 24 September 2012
Gene activation
To show the functionality of our TAL protein as well as the impact of the VP 64 transcription factor fusion protein, we used a TAL-VP64 fusion construct targetting a minimal promotor coupled with the secreated alkaline phosphatase (SEAP).
The experiment was done with four different transfections, either no plasmid, only the TAL vector, only the SEAP plasmid or a cotransfection of both plasmids.
The experiment was done with four different transfections, either no plasmid, only the TAL vector, only the SEAP plasmid or a cotransfection of both plasmids.
