http://2012.igem.org/wiki/index.php?title=Team:Freiburg/Project/Coop&feed=atom&action=historyTeam:Freiburg/Project/Coop - Revision history2024-03-28T08:06:17ZRevision history for this page on the wikiMediaWiki 1.16.0http://2012.igem.org/wiki/index.php?title=Team:Freiburg/Project/Coop&diff=290096&oldid=prevShepherd at 21:34, 26 October 20122012-10-26T21:34:36Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We are in contact with Team Fatih Medical since May and discussed exciting new applications of TAL effectors. Moreover, we have filled out their survey on synthetic biology, which is part of their human practices project.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We are in contact with Team Fatih Medical since May and discussed exciting new applications of TAL effectors. Moreover, we have filled out their survey on synthetic biology, which is part of their human practices project.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><b>Collaboration with Team Evry:</b></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">On the regional Jamboree in Amsterdam, we were fascinated by the work of the Evry iGEM Team. Using Xenopus as a model organism for synthetic biology is clearly an interesting and challenging endeavor. We got approached by Nicolas Pollet from Team Evry, who wants to apply our GATE kit in Xenopus. That is why we currently prepare to send our kit to Team Evry.</del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[#top|Back to top]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[#top|Back to top]]</div></td></tr>
</table>Shepherdhttp://2012.igem.org/wiki/index.php?title=Team:Freiburg/Project/Coop&diff=290077&oldid=prevShepherd at 21:32, 26 October 20122012-10-26T21:32:25Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b>Collaboration with Team Evry:</b></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b>Collaboration with Team Evry:</b></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>On the regional Jamboree in Amsterdam, we were fascinated by the work of the Evry iGEM Team. Using Xenopus as a model organism for synthetic biology is clearly an interesting and challenging endeavor. We got approached by Nicolas Pollet from Team Evry, who wants to apply our GATE kit in Xenopus. That is why we currently prepare to send our kit to Team Evry.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>On the regional Jamboree in Amsterdam, we were fascinated by the work of the Evry iGEM Team. Using Xenopus as a model organism for synthetic biology is clearly an interesting and challenging endeavor. We got approached by Nicolas Pollet from Team Evry, who wants to apply our GATE kit in Xenopus. That is why we currently prepare to send our kit to Team Evry.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td></tr>
</table>Shepherdhttp://2012.igem.org/wiki/index.php?title=Team:Freiburg/Project/Coop&diff=290071&oldid=prevShepherd at 21:32, 26 October 20122012-10-26T21:32:03Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b>Collaboration with Team Potsdam Bioware:</b></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b>Collaboration with Team Potsdam Bioware:</b></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Team Potsdam is working on a CHO cell-based project for generating antibodies. Hypermutation should be accomplished by cotransfection of the activation-induced cytidine deaminase (AID). Since it is obviously preferable that the activity of the AID is focussed on the antibody gene, we decided to put our expertise in our two distinct research fields together and produce a TAL-AID fusion protein that binds to the antibody gene-containing plasmid. Using the Plug-and-Play Effector Cassette (PPEC) in our eukaryotic TAL expression vector, it was quite easy to fuse the AID gene to our TAL scaffold. The next step, namely targeting the TAL-AID construct to the antibody plasmid was a child's play with our GATE kit. So we produced a TAL-AID that specifically binds to the sequence T G C G G T C T A T T A C T and currently test it in collaboration with the Potsdam Bioware Team. We hope that we will be able to show you the new data in Boston.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Team Potsdam is working on a CHO cell-based project for generating antibodies. Hypermutation should be accomplished by cotransfection of the activation-induced cytidine deaminase (AID). Since it is obviously preferable that the activity of the AID is focussed on the antibody gene, we decided to put our expertise in our two distinct research fields together and produce a TAL-AID fusion protein that binds to the antibody gene-containing plasmid. Using the Plug-and-Play Effector Cassette (PPEC) in our eukaryotic TAL expression vector, it was quite easy to fuse the AID gene to our TAL scaffold. The next step, namely targeting the TAL-AID construct to the antibody plasmid<ins class="diffchange diffchange-inline">, </ins>was a child's play with our GATE kit. So we produced a TAL-AID that specifically binds to the sequence T G C G G T C T A T T A C T and currently test it in collaboration with the Potsdam Bioware Team. We hope that we will be able to show you the new data in Boston.</div></td></tr>
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</table>Shepherdhttp://2012.igem.org/wiki/index.php?title=Team:Freiburg/Project/Coop&diff=290051&oldid=prevShepherd at 21:30, 26 October 20122012-10-26T21:30:32Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b>Collaboration with Team Potsdam Bioware:</b></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b>Collaboration with Team Potsdam Bioware:</b></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Team Potsdam is working on a CHO cell-based project for generating antibodies. Hypermutation should be accomplished by cotransfection of the activation-induced cytidine deaminase (AID). Since it is obviously preferable that the activity of the AID is focussed on the antibody gene, we decided to put our expertise in two <del class="diffchange diffchange-inline">entirely </del>distinct research fields together and produce a TAL-AID fusion protein that binds to the antibody gene-containing plasmid. <del class="diffchange diffchange-inline">Due to </del>the Plug-and-Play Effector Cassette (PPEC) in our eukaryotic TAL expression vector, it was quite easy to <del class="diffchange diffchange-inline">introduce </del>the TAL-AID <del class="diffchange diffchange-inline">vector first and </del>we <del class="diffchange diffchange-inline">are </del>currently <del class="diffchange diffchange-inline">completing </del>the <del class="diffchange diffchange-inline">order so that team </del>Potsdam <del class="diffchange diffchange-inline">can test </del>the <del class="diffchange diffchange-inline">construct</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Team Potsdam is working on a CHO cell-based project for generating antibodies. Hypermutation should be accomplished by cotransfection of the activation-induced cytidine deaminase (AID). Since it is obviously preferable that the activity of the AID is focussed on the antibody gene, we decided to put our expertise in <ins class="diffchange diffchange-inline">our </ins>two distinct research fields together and produce a TAL-AID fusion protein that binds to the antibody gene-containing plasmid. <ins class="diffchange diffchange-inline">Using </ins>the Plug-and-Play Effector Cassette (PPEC) in our eukaryotic TAL expression vector, it was quite easy to <ins class="diffchange diffchange-inline">fuse the AID gene to our TAL scaffold. The next step, namely targeting </ins>the TAL-AID <ins class="diffchange diffchange-inline">construct to the antibody plasmid was a child's play with our GATE kit. So </ins>we <ins class="diffchange diffchange-inline">produced a TAL-AID that specifically binds to the sequence T G C G G T C T A T T A C T and </ins>currently <ins class="diffchange diffchange-inline">test it in collaboration with </ins>the Potsdam <ins class="diffchange diffchange-inline">Bioware Team. We hope that we will be able to show you </ins>the <ins class="diffchange diffchange-inline">new data in Boston</ins>.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b>Collaboration with Team Fatih Medical:</b></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b>Collaboration with Team Fatih Medical:</b></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We are in contact with Team Fatih Medical since May and discussed exciting new applications of TAL effectors. Moreover, we have filled out their survey on synthetic biology, which is part of their human practices project. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We are in contact with Team Fatih Medical since May and discussed exciting new applications of TAL effectors. Moreover, we have filled out their survey on synthetic biology, which is part of their human practices project<ins class="diffchange diffchange-inline">.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><b>Collaboration with Team Evry:</b></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">On the regional Jamboree in Amsterdam, we were fascinated by the work of the Evry iGEM Team. Using Xenopus as a model organism for synthetic biology is clearly an interesting and challenging endeavor. We got approached by Nicolas Pollet from Team Evry, who wants to apply our GATE kit in Xenopus. That is why we currently prepare to send our kit to Team Evry</ins>.</div></td></tr>
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</table>Shepherdhttp://2012.igem.org/wiki/index.php?title=Team:Freiburg/Project/Coop&diff=276510&oldid=prevLuboe at 20:29, 24 October 20122012-10-24T20:29:45Z<p></p>
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</table>Luboehttp://2012.igem.org/wiki/index.php?title=Team:Freiburg/Project/Coop&diff=276509&oldid=prevLuboe at 20:29, 24 October 20122012-10-24T20:29:34Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We are in contact with Team Fatih Medical since May and discussed exciting new applications of TAL effectors. Moreover, we have filled out their survey on synthetic biology, which is part of their human practices project. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We are in contact with Team Fatih Medical since May and discussed exciting new applications of TAL effectors. Moreover, we have filled out their survey on synthetic biology, which is part of their human practices project. </div></td></tr>
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</table>Luboehttp://2012.igem.org/wiki/index.php?title=Team:Freiburg/Project/Coop&diff=271340&oldid=prevPablinitus at 12:32, 19 October 20122012-10-19T12:32:12Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>= Collaborations =</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>= Collaborations =</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br>[[File:<del class="diffchange diffchange-inline">attributionssymbol</del>.png|center|180px|link=]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br>[[File:<ins class="diffchange diffchange-inline">attributionssymbolT</ins>.png|center|180px|link=]]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div align="justify">During the course of our iGEM project and especally after [[Team:Freiburg/HumanPractices/Outreach#Poster|meeting]] other iGEM teams in berlin, the news about our toolkit spread through Europe and we got in contact with some other teams that were interested in using TAL effectors in their projects.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div align="justify">During the course of our iGEM project and especally after [[Team:Freiburg/HumanPractices/Outreach#Poster|meeting]] other iGEM teams in berlin, the news about our toolkit spread through Europe and we got in contact with some other teams that were interested in using TAL effectors in their projects.</div></td></tr>
</table>Pablinitushttp://2012.igem.org/wiki/index.php?title=Team:Freiburg/Project/Coop&diff=271200&oldid=prevLuboe at 07:55, 19 October 20122012-10-19T07:55:16Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>= Collaborations =</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>= Collaborations =</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>----</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>----</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br>[[File:attributionssymbol.png|center|180px]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br>[[File:attributionssymbol.png|center|180px<ins class="diffchange diffchange-inline">|link=</ins>]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div align="justify">During the course of our iGEM project and especally after [[Team:Freiburg/HumanPractices/Outreach#Poster|meeting]] other iGEM teams in berlin, the news about our toolkit spread through Europe and we got in contact with some other teams that were interested in using TAL effectors in their projects.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div align="justify">During the course of our iGEM project and especally after [[Team:Freiburg/HumanPractices/Outreach#Poster|meeting]] other iGEM teams in berlin, the news about our toolkit spread through Europe and we got in contact with some other teams that were interested in using TAL effectors in their projects.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[File:teamsgerm_SW.jpg|center|400px]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[File:teamsgerm_SW.jpg|center|400px<ins class="diffchange diffchange-inline">|link=</ins>]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Luboehttp://2012.igem.org/wiki/index.php?title=Team:Freiburg/Project/Coop&diff=268710&oldid=prevPablinitus: /* Collaborations */2012-10-10T16:07:04Z<p><span class="autocomment">Collaborations</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 16:07, 10 October 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 5:</td>
<td colspan="2" class="diff-lineno">Line 5:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>[[File:attributionssymbol.png|center|180px]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>[[File:attributionssymbol.png|center|180px]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>During the course of our iGEM project and especally after [[Team:Freiburg/HumanPractices/Outreach#Poster|meeting]] other iGEM teams in berlin, the news about our toolkit spread through Europe and we got in contact with some other teams that were interested in using TAL effectors in their projects.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><div align="justify"></ins>During the course of our iGEM project and especally after [[Team:Freiburg/HumanPractices/Outreach#Poster|meeting]] other iGEM teams in berlin, the news about our toolkit spread through Europe and we got in contact with some other teams that were interested in using TAL effectors in their projects.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:teamsgerm_SW.jpg|center|400px]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:teamsgerm_SW.jpg|center|400px]]</div></td></tr>
</table>Pablinitushttp://2012.igem.org/wiki/index.php?title=Team:Freiburg/Project/Coop&diff=268700&oldid=prevPablinitus: /* Collaborations */2012-10-10T15:14:57Z<p><span class="autocomment">Collaborations</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 15:14, 10 October 2012</td>
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<td colspan="2" class="diff-lineno">Line 3:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>= Collaborations =</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>= Collaborations =</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>----</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>----</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br>[[File:attributionssymbol.png|center|180px]]</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>During the course of our iGEM project and especally after [[Team:Freiburg/HumanPractices/Outreach#Poster|meeting]] other iGEM teams in berlin, the news about our toolkit spread through Europe and we got in contact with some other teams that were interested in using TAL effectors in their projects.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>During the course of our iGEM project and especally after [[Team:Freiburg/HumanPractices/Outreach#Poster|meeting]] other iGEM teams in berlin, the news about our toolkit spread through Europe and we got in contact with some other teams that were interested in using TAL effectors in their projects.</div></td></tr>
</table>Pablinitus