Team:Exeter/lab book/novpol/wk9
From 2012.igem.org
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<p>**<b>Friday 07/09/12</b>**</p><br> | <p>**<b>Friday 07/09/12</b>**</p><br> | ||
<p><b>11.00am</b></p> | <p><b>11.00am</b></p> | ||
- | <p>Gel electrophoresis run</p> | + | <p>Gel electrophoresis run - insert image here</p> |
+ | <br><br> | ||
Revision as of 10:28, 13 September 2012
Showcasing Polysaccharide Production: 3rd - 7th September 2012 **Monday 03/09/12** 9.00am Transformations using Gene_Terminator.
Plasmid + Upstream + Downstream Plasmid + Tetr_RBS + Cyc_Term Plasmid + Tetr_RBS + HAS_Term Plasmid + OmpA + SacB_Term 11.20am All in the incubator for digestion period. 12.40pm Heat denatured enzymes at 80°C for 20 minutes. 1.00pm Ligation process
2.45pm Currently no enzymes, all DNA into freezer. 4.30pm Streak plates made from the single colonies that appeared to have worked. Colonies that form should therefore also work.
All into incubator at 37°C overnight. **Thursday 06/09/12** 3.30pm Digestion
Into incubator for overnight digestion. **Friday 07/09/12** 11.00am Gel electrophoresis run - insert image here Sequences confirmed for:
Single genes confirmed for:
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