Team:Exeter/lab book/3gip/wk11

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     <p><b><u>Monday 15th September</u></b></p>
     <p><b><u>Monday 15th September</u></b></p>
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<p><u><i>Disaster!</i></u> I had not heat deactivated the enzymes after digestion so I had to perform the <a href="https://2012.igem.org/Team:Exeter/lab_book/proto/4" style="color:#1d1d1b"><u>transfer of the BioBricks</u></a> into pSB1C3 again. A repeat of Fridays lab but the ligation was left overnight in the fridge. </p><br>
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<p><u><i>Disaster!</i></u> I had not heat deactivated the enzymes after digestion so I had to perform the <a href="https://2012.igem.org/Team:Exeter/lab_book/proto/4" style="color:#57b947"><u>transfer of the BioBricks</u></a> into pSB1C3 again. A repeat of Fridays lab but the ligation was left overnight in the fridge. </p><br>
<p><b><u>Tuesday 16th September</u></b></p>
<p><b><u>Tuesday 16th September</u></b></p>

Revision as of 15:28, 26 September 2012

ExiGEM2012 Lab Book 3GP wk6

The 3-Gene Inducible Plasmid: 17th - 21st September 2012

Monday 15th September

Disaster! I had not heat deactivated the enzymes after digestion so I had to perform the transfer of the BioBricks into pSB1C3 again. A repeat of Fridays lab but the ligation was left overnight in the fridge.


Tuesday 16th September

Today I was handing the lab work over to Alex. Please see Single Gene Plasmids and Enzyme Characterisation: 24th - 28th September 2012