Team:Exeter/lab book/1gp/wk7

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ExiGEM2012 Lab Book 1GP wk7


Protocols \| Single Gene Plasmids and Enzyme Characterisation \| Showcasing Polysaccharide Production \| The 3-Gene Inducible Plasmid Operon Construction \| Glycobase
9th - 13th July - 16th - 20th July - 23rd - 27th July - 30th July - 3rd August - 6th - 10th August - 13th - 17th August - 20th - 24th August - 17th - 21st September - 24th - 28th September - Results
	Single Gene Plasmids and Enzyme Characterisation: 20th - 24th August 2012 Monday 20th August (11.00) • 3A assembly of WbnJ-pSB1C3, WbnK-pSB1C3, WbbC(with point mutation)-pSB1C3, WfcA-pSB1C3, WclY-pSB1C3 and WbiP-pSB1C3 Tuesday 21st August (9.00) • Transformation of WbnJ-pSB1C3, WbnK-pSB1C3, WbbC(with point mutation)-pSB1C3, WfcA-pSB1C3, WclY-pSB1C3 and WbiP-pSB1C3 Wednesday 22nd August (9.00) • 3A assembly of WclY-pSB1C3 and WbiP-pSB1C3 • Adding cultures with ligated WbnJ-pSB1C3, WbnK-pSB1C3, WbbC(with point mutation)-pSB1C3 and WfcA-pSB1C3 as well as WbbC(with point mutation)-terminator into liquid medium overnight No colonies with ligated WclY-pSB1C3 and WbiP-pSB1C3 were found and thus 3A assembly was repeated. Thursday 23rd August (9.00) • Adding cultures with ligated WbnJ-pSB1C3, WbnK-pSB1C3, WbbC(with point mutation)-pSB1C3 and WfcA-pSB1C3 as well as WbbC(with point mutation)-terminator into liquid medium overnight • Transformation of WclY-pSB1C3 and WbiP-pSB1C3 The shaking incubator broke on Wednesday evening and therefore adding cultures into liquid medium was repeated.