Team:Exeter/lab book/1gp/wk7

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       <a href="https://2012.igem.org/Team:Exeter/lab_book/1gp/wk1"; style="color:#57b947">Single Gene Plasmids and Enzyme Characterisation</a>
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      <a href="https://2012.igem.org/Team:Exeter/lab_book/proto"; style="color:#57b947">Protocols</a>
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      <a href="https://2012.igem.org/Team:Exeter/lab_book/novpol/wk1"; style="color:#57b947">Showcasing Polysaccharide Production</a>
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      <a href="https://2012.igem.org/Team:Exeter/lab_book/1gp/wk1"; style="color:#57b947">Single Gene Plasmids and Enzyme Characterisation</a>
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      <a href="https://2012.igem.org/Team:Exeter/lab_book/3gip/wk1"; style="color:#57b947">The 3-Gene Inducible Plasmid</a>
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      <a href="https://2012.igem.org/Team:Exeter/lab_book/novpol/wk1"; style="color:#57b947">Showcasing Polysaccharide Production</a>
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      <a href="https://2012.igem.org/Team:Exeter/lab_book/gibs/wk1"; style="color:#57b947">Operon Construction</a>       
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      <a href="https://2012.igem.org/Team:Exeter/lab_book/3gip/wk1"; style="color:#57b947">The 3-Gene Inducible Plasmid</a>
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      <a href="https://2012.igem.org/Team:Exeter/lab_book/glyco/wk1"; style="color:#57b947">Glycobase</a>
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      <a href="https://2012.igem.org/Team:Exeter/lab_book/gibs/wk1"; style="color:#57b947">Operon Construction</a>       
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      <a href="https://2012.igem.org/Team:Exeter/lab_book/glyco/wk1"; style="color:#57b947">Glycobase</a>
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Revision as of 15:15, 19 September 2012

ExiGEM2012 Lab Book 1GP wk7

Single Gene Plasmids and Enzyme Characterisation: 20th - 24th August 2012

Monday 20th August (11.00)

• 3A assembly of WbnJ-pSB1C3, WbnK-pSB1C3, WbbC(with point mutation)-pSB1C3, WfcA-pSB1C3, WclY-pSB1C3 and WbiP-pSB1C3

Tuesday 21st August (9.00)

• Transformation of WbnJ-pSB1C3, WbnK-pSB1C3, WbbC(with point mutation)-pSB1C3, WfcA-pSB1C3, WclY-pSB1C3 and WbiP-pSB1C3

Wednesday 22nd August (9.00)

• 3A assembly of WclY-pSB1C3 and WbiP-pSB1C3

• Adding cultures with ligated WbnJ-pSB1C3, WbnK-pSB1C3, WbbC(with point mutation)-pSB1C3 and WfcA-pSB1C3 as well as WbbC(with point mutation)-terminator into liquid medium overnight

No colonies with ligated WclY-pSB1C3 and WbiP-pSB1C3 were found and thus 3A assembly was repeated.

Thursday 23rd August (9.00)

• Adding cultures with ligated WbnJ-pSB1C3, WbnK-pSB1C3, WbbC(with point mutation)-pSB1C3 and WfcA-pSB1C3 as well as WbbC(with point mutation)-terminator into liquid medium overnight

• Transformation of WclY-pSB1C3 and WbiP-pSB1C3

The shaking incubator broke on Wednesday evening and therefore adding cultures into liquid medium was repeated.

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