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Team:Exeter/lab book/1gp/wk2
From 2012.igem.org
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<b><u>Monday 16th July (11.00)</b></u></p> | <b><u>Monday 16th July (11.00)</b></u></p> | ||
| - | <p>• <a href="https://2012.igem.org/Team:Exeter/lab_book/proto/6" style="color:# | + | <p>• <a href="https://2012.igem.org/Team:Exeter/lab_book/proto/6" style="color:#57b947"><u>BioBrick extraction</u></a> of RBS BioBrick (BBa_B0034) and TetR repressible promoter (BBa_R0040) |
| - | <p>• <a href="https://2012.igem.org/Team:Exeter/lab_book/proto/1" style="color:# | + | <p>• <a href="https://2012.igem.org/Team:Exeter/lab_book/proto/1" style="color:#57b947"><u>Transformation</u></a> of RBS BioBrick and TetR promoter</p> |
<p><b><u>Tuesday 17th July (15.00)</b></u></p> | <p><b><u>Tuesday 17th July (15.00)</b></u></p> | ||
| - | <p>• <a href="https://2012.igem.org/Team:Exeter/lab_book/proto/2" style="color:# | + | <p>• <a href="https://2012.igem.org/Team:Exeter/lab_book/proto/2" style="color:#57b947"><u>Adding cultures</u></a> with RBS and TetR promoter into liquid medium and incubation overnight</p> |
<p> Protocol was followed using ampicillin for all BioBrick parts as the selection antibiotic.</p> | <p> Protocol was followed using ampicillin for all BioBrick parts as the selection antibiotic.</p> | ||
<p><b><u>Wednesday 18th July (15.00)</b></u></p> | <p><b><u>Wednesday 18th July (15.00)</b></u></p> | ||
| - | <p>• <a href="https://2012.igem.org/Team:Exeter/lab_book/proto/2" style="color:# | + | <p>• <a href="https://2012.igem.org/Team:Exeter/lab_book/proto/2" style="color:#57b947"><u>Adding cultures</u></a> with RBS and TetR promoter into liquid medium and incubation overnight</p> |
<p> No cultures were found in all liquid mediums and thus repeated.</p> | <p> No cultures were found in all liquid mediums and thus repeated.</p> | ||
<p><b><u>Thursday 18th July (9.00)</b></u></p> | <p><b><u>Thursday 18th July (9.00)</b></u></p> | ||
| - | <p>• <a href="http://www.fermentas.com/templates/files/tiny_mce/coa_pdf/coa_k0502.pdf" style="color:# | + | <p>• <a href="http://www.fermentas.com/templates/files/tiny_mce/coa_pdf/coa_k0502.pdf" style="color:#57b947"><u>Mini-Prepping</u></a> of RBS and TetR promoter |
<p>• Gel Electrophoresis to check fragment sizes of RBS and TetR promoter | <p>• Gel Electrophoresis to check fragment sizes of RBS and TetR promoter | ||
<p> Gel Electrophoresis showed that RBS and TetR promoter were successfully cloned. <b>(bands???)</b></p> | <p> Gel Electrophoresis showed that RBS and TetR promoter were successfully cloned. <b>(bands???)</b></p> | ||
Revision as of 10:48, 26 September 2012
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Single Gene Plasmids and Enzyme Characterisation: 16th - 20th July 2012 Monday 16th July (11.00)• BioBrick extraction of RBS BioBrick (BBa_B0034) and TetR repressible promoter (BBa_R0040) • Transformation of RBS BioBrick and TetR promoter Tuesday 17th July (15.00) • Adding cultures with RBS and TetR promoter into liquid medium and incubation overnight Protocol was followed using ampicillin for all BioBrick parts as the selection antibiotic. Wednesday 18th July (15.00) • Adding cultures with RBS and TetR promoter into liquid medium and incubation overnight No cultures were found in all liquid mediums and thus repeated. Thursday 18th July (9.00) • Mini-Prepping of RBS and TetR promoter • Gel Electrophoresis to check fragment sizes of RBS and TetR promoter Gel Electrophoresis showed that RBS and TetR promoter were successfully cloned. (bands???)
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