Team:Exeter/lab book/1gp/wk2

From 2012.igem.org

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       <a href="https://2012.igem.org/Team:Exeter/lab_book/1gp/wk1"; style="color:#57b947">Single Gene Plasmids and Enzyme Characterisation</a>
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      <a href="https://2012.igem.org/Team:Exeter/lab_book/proto"; style="color:#57b947">Protocols</a>
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      <a href="https://2012.igem.org/Team:Exeter/lab_book/3gip/wk1"; style="color:#57b947">The 3-Gene Inducible Plasmid</a>
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      <a href="https://2012.igem.org/Team:Exeter/lab_book/gibs/wk1"; style="color:#57b947">Operon Construction</a>       
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      <a href="https://2012.igem.org/Team:Exeter/lab_book/3gip/wk1"; style="color:#57b947">The 3-Gene Inducible Plasmid</a>
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      <a href="https://2012.igem.org/Team:Exeter/lab_book/glyco/wk1"; style="color:#57b947">Glycobase</a>
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      <a href="https://2012.igem.org/Team:Exeter/lab_book/glyco/wk1"; style="color:#57b947">Glycobase</a>
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Revision as of 15:14, 19 September 2012

ExiGEM2012 Lab Book 1GP wk2

Single Gene Plasmids and Enzyme Characterisation: 16th - 20th July 2012

 Monday 16th July (11.00)

• BioBrick extraction of RBS BioBrick (BBa_B0034) and TetR repressible promoter (BBa_R0040)

• Transformation of RBS BioBrick and TetR promoter

Tuesday 17th July (15.00)

• Adding cultures with RBS and TetR promoter into liquid medium and incubation overnight

Protocol was followed using ampicillin for all BioBrick parts as the selection antibiotic.

Wednesday 18th July (15.00)

• Adding cultures with RBS and TetR promoter into liquid medium and incubation overnight

No cultures were found in all liquid mediums and thus repeated.

Thursday 18th July (9.00)

• Mini-Prepping of RBS and TetR promoter

• Gel Electrophoresis to check fragment sizes of RBS and TetR promoter

Gel Electrophoresis showed that RBS and TetR promoter were successively cloned.

Retrieved from "http://2012.igem.org/Team:Exeter/lab_book/1gp/wk2"