Team:Exeter/Diary/wk6

From 2012.igem.org

(Difference between revisions)
 
(14 intermediate revisions not shown)
Line 9: Line 9:
<body>
<body>
-
  <table width="1075" border="0" cellpadding="10">
+
  <table width="980" border="0" cellpadding="10">
   
   
  <!--Spacer for Menu Banner-->
  <!--Spacer for Menu Banner-->
Line 17: Line 17:
  <!--End of Spacer-->
  <!--End of Spacer-->
-
<!--Diary Navigation-->
+
  <!--Diary Navigation-->
   <tr>
   <tr>
   <td colspan="2" align="center">
   <td colspan="2" align="center">
     <div style="text-align:center" >
     <div style="text-align:center" >
-
     <font face="DokChampa" color="#57b947" size="3">
+
     <font face="Verdana" color="#57b947" size="2">
       <p>
       <p>
       <a href="https://2012.igem.org/Team:Exeter/Diary"; style="color:#57b947">Week T-Minus 2</a>
       <a href="https://2012.igem.org/Team:Exeter/Diary"; style="color:#57b947">Week T-Minus 2</a>
Line 29: Line 29:
       <a href="https://2012.igem.org/Team:Exeter/Diary/wk0"; style="color:#57b947">Week 0</a>
       <a href="https://2012.igem.org/Team:Exeter/Diary/wk0"; style="color:#57b947">Week 0</a>
       </p>
       </p>
 +
      <p>
       <a href="https://2012.igem.org/Team:Exeter/Diary/wk1"; style="color:#57b947">Week 1</a>
       <a href="https://2012.igem.org/Team:Exeter/Diary/wk1"; style="color:#57b947">Week 1</a>
         &nbsp;|&nbsp;
         &nbsp;|&nbsp;
Line 48: Line 49:
         &nbsp;|&nbsp;
         &nbsp;|&nbsp;
       <a href="https://2012.igem.org/Team:Exeter/Diary/wk10"; style="color:#57b947">Week 10</a>
       <a href="https://2012.igem.org/Team:Exeter/Diary/wk10"; style="color:#57b947">Week 10</a>
 +
        &nbsp;|&nbsp;
 +
      <a href="https://2012.igem.org/Team:Exeter/Diary/wk11"; style="color:#57b947">Week 11</a>
 +
        &nbsp;|&nbsp;
 +
      <a href="https://2012.igem.org/Team:Exeter/Diary/wk12"; style="color:#57b947">Week 12</a>
 +
      </p>
       </font>
       </font>
     </div>
     </div>
Line 53: Line 59:
   </tr>
   </tr>
  <!--End Diary Navigation-->
  <!--End Diary Navigation-->
-
 
  <!--Weekly Image-->
  <!--Weekly Image-->
   <tr>
   <tr>
   <td colspan="2">
   <td colspan="2">
-
     <img src=""alt="" title="" width="1075px" height="250px">
+
     <img src="https://static.igem.org/mediawiki/2012/e/ef/Exe2012_Week_6_Diary_Banner.jpg"alt="" title="" width="980px" height="228px">
   </td>
   </td>
   </tr>
   </tr>
Line 66: Line 71:
   <td>
   <td>
     <div style="text-align:justify">
     <div style="text-align:justify">
-
     <font face="DokChampa" color="#1d1d1b" size="2">  
+
     <font face="Verdana" color="#1d1d1b" size="2">  
      
      
-
       <font face="DokChampa" color="#57b947" size="4">
+
       <font face="Verdana" color="#57b947" size="4">
       <p><b>WEEK SIX</b></p>
       <p><b>WEEK SIX</b></p>
       </font>
       </font>
      
      
-
       <p><u><big>23rd-27th July 2012</big></u></p>
+
       <p><u><big>23rd-27th July 2012</big></u></p><br>
        
        
       <!-------------ENTER TEXT HERE------------->
       <!-------------ENTER TEXT HERE------------->
        
        
 +
      <p>Initially the entire wet lab team worked on bulking up our <i>wbnJ</i> and <i>wfcA</i> genes. They also did 3A assembly of some promoters to RBS sequences - this sadly didn't work.</p>
 +
<p>However a persistent Mary and Freddie attempted the 3A assembly for a second time alongside the standard assembly. The resulting product was transformed and run on a gel. This gel disappointingly showed no fragments other than the plasmid after digestion, however transformation in antibiotic medium did work. This led to the realisation that the promoter biobricks were smaller than we had first believed and therefore wouldn’t appear clearly on the gel. The plasmid was sent off for sequencing and another gel run. This second gel showed two bands, consistent with what you would expect from the two biobricks together alongside the plasmid backbone. Hopefully our sequencing results will confirm it was indeed successful!</p><br>
 +
 +
      <p> Our wiki team have all been coding using the Windows operating system (don't ask why) and so were shocked to discover that Macs were displaying an entirely different font to the one the team had chosen! Ryan had been convinced that our initial font was universal and <i>'web safe'</i>.. it turns out not to have been the case! With the chief wiki coder away it fell to Alex to 'sort it out'.</p>
 +
      <p>And so it was, that this week would see all the pages change to endure the long lasting privilege of the 'Verdana' font type - which is definitely universal and <i>'web safe'</i>!</p><br>
 +
 +
      <!The wetlab teams have seen some ups and downs this week, but they are certainly ending on a high this Friday!>
 +
 +
      <p>This week the team has been busy preparing for a human practices presentation due to be given Friday 3rd August. In this talk we'll be tackling the key issues we identified revolving around the ethical, environmental, and potential business impacts our project may have. We have invited five professionals in their respective fields to sit on a designated panel, they will have the opportunity to ask us relevant questions and a general audience will also be present.</p>
 +
      <p>Wish us luck, you'll be able to see how we got on <font face="Verdana" color="#57b947" size="2">
 +
      <b><u><a href="https://2012.igem.org/Team:Exeter/Human_Practices/panel"; style="color:#57b947" target="_blank">here</a></u></b></font>).</p>
 +
     </font>
     </font>
     </div>
     </div>
Line 83: Line 100:
</table>
</table>
-
 
+
<table width="980" align="center" cellspacing="20">
 +
<tr align="center">
 +
  <td>
 +
  <font color="#57B947" size="1" face="Verdana">
 +
    <p><u>Website Designed and Built by: Ryan Edginton, James Lynch & Alex Clowsley</u> &nbsp;&nbsp;|&nbsp;&nbsp;
 +
    <a href="https://igem.org/Team.cgi?id=764" style="color:#57B947" target="_blank"><u>Contact Us</u></a>  &nbsp;&nbsp;|&nbsp;&nbsp;
 +
    <a href="https://2012.igem.org/Team:Exeter/site_map" style="color:#57B947"><u>Site Map</u></a></p>
 +
  </font>
 +
  </td>
 +
</tr>
 +
</table>
</body>
</body>
</html>
</html>

Latest revision as of 00:09, 27 September 2012

ExiGEM2012 Diary Week 6

Week T-Minus 2  |  Week T-Minus 1  |  Week 0

Week 1  |  Week 2  |  Week 3  |  Week 4  |  Week 5  |  Week 6  |  Week 7  |  Week 8  |  Week 9  |  Week 10  |  Week 11  |  Week 12

WEEK SIX

23rd-27th July 2012


Initially the entire wet lab team worked on bulking up our wbnJ and wfcA genes. They also did 3A assembly of some promoters to RBS sequences - this sadly didn't work.

However a persistent Mary and Freddie attempted the 3A assembly for a second time alongside the standard assembly. The resulting product was transformed and run on a gel. This gel disappointingly showed no fragments other than the plasmid after digestion, however transformation in antibiotic medium did work. This led to the realisation that the promoter biobricks were smaller than we had first believed and therefore wouldn’t appear clearly on the gel. The plasmid was sent off for sequencing and another gel run. This second gel showed two bands, consistent with what you would expect from the two biobricks together alongside the plasmid backbone. Hopefully our sequencing results will confirm it was indeed successful!


Our wiki team have all been coding using the Windows operating system (don't ask why) and so were shocked to discover that Macs were displaying an entirely different font to the one the team had chosen! Ryan had been convinced that our initial font was universal and 'web safe'.. it turns out not to have been the case! With the chief wiki coder away it fell to Alex to 'sort it out'.

And so it was, that this week would see all the pages change to endure the long lasting privilege of the 'Verdana' font type - which is definitely universal and 'web safe'!


This week the team has been busy preparing for a human practices presentation due to be given Friday 3rd August. In this talk we'll be tackling the key issues we identified revolving around the ethical, environmental, and potential business impacts our project may have. We have invited five professionals in their respective fields to sit on a designated panel, they will have the opportunity to ask us relevant questions and a general audience will also be present.

Wish us luck, you'll be able to see how we got on here).

Website Designed and Built by: Ryan Edginton, James Lynch & Alex Clowsley   |   Contact Us   |   Site Map