Team:Exeter

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	Project Abstract: Our aim is to engineer Escherichia coli (E. coli) tosynthesise a novel polysaccharide determined by us. We aim also to construct an engineered plasmid which will allow the expression of different enzymes, producing the polysaccharides chosen depending on the stimulus of particular promoters. Thus giving a controlled external selection mechanism to the polysaccharide production of the cell. To achieve the initial novel polysaccharide, we shall firstly implement the addition of glycosyltransferases from different E. Coli strains to our E. Coli allowing production of polysaccharides by the well documented Wzy-Wzz method. The ultimate goal of this engineering is to demonstrate the production of desired polysaccharides. More over we provide a mechanism by which polysaccharides can be produced 'to order'. The applications for this means are enormous. For example vaccines could be produced at speed upon an epidemic. Material scientists and engineers would be able to choose a synthetic polysaccharide fulfilling desired properties.