http://2012.igem.org/wiki/index.php?title=Team:Evry/TeamSlovenia_collaboration&feed=atom&action=historyTeam:Evry/TeamSlovenia collaboration - Revision history2024-03-29T12:25:07ZRevision history for this page on the wikiMediaWiki 1.16.0http://2012.igem.org/wiki/index.php?title=Team:Evry/TeamSlovenia_collaboration&diff=293899&oldid=prevTriCer at 01:03, 27 October 20122012-10-27T01:03:37Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To test our chassis we decided to do a collaboration with a team working in vertebrate cells.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To test our chassis we decided to do a collaboration with a team working in vertebrate cells.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>After the Regional Jamboree, and the presentation of <a href="https://2012.igem.org/Team:Slovenia">Team Slovenia</a> during the final, we asked them to test one of their construction. Especially constructions using <del class="diffchange diffchange-inline"><a href="https://2012.igem.org/Team:Slovenia/TheSwitchDesignedTALregulators"></del>TAL activator constructs<del class="diffchange diffchange-inline"></a> </del>(basement of their switch system).</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>After the Regional Jamboree, and the presentation of <a href="https://2012.igem.org/Team:Slovenia">Team Slovenia</a> during the final, we asked them to test one of their construction. Especially constructions using TAL activator constructs (basement of their switch system).</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The mCitrine were still expressed 3 days after injection, and tadpoles were developing. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The mCitrine were still expressed 3 days after injection, and tadpoles were developing. </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Details of this system is visible <a href="https://2012.igem.org/Team:Slovenia/TheSwitchDesignedTALregulators">here</a> (Team Slovenia page).</ins></div></td></tr>
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</table>TriCerhttp://2012.igem.org/wiki/index.php?title=Team:Evry/TeamSlovenia_collaboration&diff=293813&oldid=prevTriCer at 01:00, 27 October 20122012-10-27T01:00:31Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To test our chassis we decided to do a collaboration with a team working in vertebrate cells.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To test our chassis we decided to do a collaboration with a team working in vertebrate cells.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>After the Regional Jamboree, and the presentation of Team Slovenia during the final, we asked them to test one of their construction. Especially constructions using TAL activator constructs (basement of their switch system).</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>After the Regional Jamboree, and the presentation of <ins class="diffchange diffchange-inline"><a href="https://2012.igem.org/Team:Slovenia"></ins>Team Slovenia<ins class="diffchange diffchange-inline"></a> </ins>during the final, we asked them to test one of their construction. Especially constructions using <ins class="diffchange diffchange-inline"><a href="https://2012.igem.org/Team:Slovenia/TheSwitchDesignedTALregulators"></ins>TAL activator constructs<ins class="diffchange diffchange-inline"></a> </ins>(basement of their switch system).</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p><del class="diffchange diffchange-inline">We received parts </del><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K782029">BBa_K782029</a> in the eukaryotic plasmid pCDNA3, and part <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K782065">BBa_K782065</a> in the eukaryotic plasmid pGL4.16.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p><ins class="diffchange diffchange-inline">So they send us part </ins><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K782029">BBa_K782029</a> in the eukaryotic plasmid pCDNA3, and part <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K782065">BBa_K782065</a> in the eukaryotic plasmid pGL4.16.</div></td></tr>
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</table>TriCerhttp://2012.igem.org/wiki/index.php?title=Team:Evry/TeamSlovenia_collaboration&diff=293240&oldid=prevTriCer at 00:30, 27 October 20122012-10-27T00:30:18Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We injected plasmids with rhodamine to be sure that embryos were injected.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We injected plasmids with rhodamine to be sure that embryos were injected.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The result show that with only the plasmid with the reporter under the minimal promoter, mCitrine is not expressed. But with the co-injection of this plasmid and the plasmid with TALA-VP16 under the control of the CMV promoter, mCitrine is expressed. It means that the switch works and the TALA-VP16 trigger the expression of mCitrie when TALA-VP16 bind the recognition site for TALA in the plasmid with mCitrine.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The result show that with only the plasmid with the reporter under the minimal promoter, mCitrine is not expressed. But with the co-injection of this plasmid and the plasmid with TALA-VP16 under the control of the CMV promoter, mCitrine is expressed. It means that the switch works and the TALA-VP16 trigger the expression of mCitrie when TALA-VP16 bind the recognition site for TALA in the plasmid with mCitrine.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"></br></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The mCitrine <del class="diffchange diffchange-inline">is </del>still expressed 3 days after injection, and <del class="diffchange diffchange-inline">the tadpole is </del>developing. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The mCitrine <ins class="diffchange diffchange-inline">were </ins>still expressed 3 days after injection, and <ins class="diffchange diffchange-inline">tadpoles were </ins>developing. </div></td></tr>
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</table>TriCerhttp://2012.igem.org/wiki/index.php?title=Team:Evry/TeamSlovenia_collaboration&diff=293212&oldid=prevTriCer at 00:29, 27 October 20122012-10-27T00:29:02Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We injected plasmids with rhodamine to be sure that embryos were injected.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We injected plasmids with rhodamine to be sure that embryos were injected.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The result show that with only the plasmid with the reporter under the minimal promoter, mCitrine is not expressed. But with the co-injection of this plasmid and the plasmid with TALA-VP16 under the control of the CMV promoter, mCitrine is expressed. It means that the switch works and the TALA-VP16 trigger the expression of mCitrie when TALA-VP16 bind the recognition site for TALA in the plasmid with mCitrine.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The result show that with only the plasmid with the reporter under the minimal promoter, mCitrine is not expressed. But with the co-injection of this plasmid and the plasmid with TALA-VP16 under the control of the CMV promoter, mCitrine is expressed. It means that the switch works and the TALA-VP16 trigger the expression of mCitrie when TALA-VP16 bind the recognition site for TALA in the plasmid with mCitrine.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">The mCitrine is still expressed 3 days after injection, and the tadpole is developing. </ins></div></td></tr>
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</table>TriCerhttp://2012.igem.org/wiki/index.php?title=Team:Evry/TeamSlovenia_collaboration&diff=293182&oldid=prevTriCer at 00:27, 27 October 20122012-10-27T00:27:17Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In a second hand both plasmids, <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K782029">BBa_K782029</a> and <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K782065">BBa_K782065</a> were co-injected. The TALA VP16 expressed with the CMV promoter bind the 10x[A] sequence of the second plasmid and over-express mCitrine.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In a second hand both plasmids, <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K782029">BBa_K782029</a> and <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K782065">BBa_K782065</a> were co-injected. The TALA VP16 expressed with the CMV promoter bind the 10x[A] sequence of the second plasmid and over-express mCitrine.</div></td></tr>
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</table>TriCerhttp://2012.igem.org/wiki/index.php?title=Team:Evry/TeamSlovenia_collaboration&diff=293134&oldid=prevTriCer at 00:24, 27 October 20122012-10-27T00:24:15Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2012/f/fc/Injection_mcitrineTeamSlovenia.png" width=800px></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2012/f/fc/Injection_mcitrineTeamSlovenia.png" width=800px></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We injected plasmids with rhodamine to be sure that embryos were injected.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We injected plasmids with rhodamine to be sure that embryos were injected.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The result show that with only the plasmid with the reporter under the minimal promoter, mCitrine is not expressed. But with the co-injection of this plasmid and the plasmid with TALA-VP16 under the control of the CMV promoter, mCitrine is expressed. It means that the switch works and the TALA-VP16 trigger the expression of mCitrie when TALA-VP16 bind the recognition site for TALA in the plasmid with mCitrine.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The result show that with only the plasmid with the reporter under the minimal promoter, mCitrine is not expressed. But with the co-injection of this plasmid and the plasmid with TALA-VP16 under the control of the CMV promoter, mCitrine is expressed. It means that the switch works and the TALA-VP16 trigger the expression of mCitrie when TALA-VP16 bind the recognition site for TALA in the plasmid with mCitrine.</div></td></tr>
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</table>TriCerhttp://2012.igem.org/wiki/index.php?title=Team:Evry/TeamSlovenia_collaboration&diff=293121&oldid=prevTriCer at 00:23, 27 October 20122012-10-27T00:23:44Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2012/f/fc/Injection_mcitrineTeamSlovenia.png" width=800px></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2012/f/fc/Injection_mcitrineTeamSlovenia.png" width=800px></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">We injected plasmids with rhodamine to be sure that embryos were injected.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">The result show that with only the plasmid with the reporter under the minimal promoter, mCitrine is not expressed. But with the co-injection of this plasmid and the plasmid with TALA-VP16 under the control of the CMV promoter, mCitrine is expressed. It means that the switch works and the TALA-VP16 trigger the expression of mCitrie when TALA-VP16 bind the recognition site for TALA in the plasmid with mCitrine.</ins></div></td></tr>
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</table>TriCerhttp://2012.igem.org/wiki/index.php?title=Team:Evry/TeamSlovenia_collaboration&diff=293035&oldid=prevTriCer at 00:18, 27 October 20122012-10-27T00:18:51Z<p></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">Revision as of 00:18, 27 October 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In a second hand both plasmids, <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K782029">BBa_K782029</a> and <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K782065">BBa_K782065</a> were co-injected. The TALA VP16 expressed with the CMV promoter bind the 10x[A] sequence of the second plasmid and over-express mCitrine.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In a second hand both plasmids, <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K782029">BBa_K782029</a> and <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K782065">BBa_K782065</a> were co-injected. The TALA VP16 expressed with the CMV promoter bind the 10x[A] sequence of the second plasmid and over-express mCitrine.</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2012/f/fc/Injection_mcitrineTeamSlovenia.png" width=<del class="diffchange diffchange-inline">450px</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2012/f/fc/Injection_mcitrineTeamSlovenia.png" width=<ins class="diffchange diffchange-inline">800px</ins>></div></td></tr>
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</table>TriCerhttp://2012.igem.org/wiki/index.php?title=Team:Evry/TeamSlovenia_collaboration&diff=293033&oldid=prevTriCer at 00:18, 27 October 20122012-10-27T00:18:37Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In a second hand both plasmids, <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K782029">BBa_K782029</a> and <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K782065">BBa_K782065</a> were co-injected. The TALA VP16 expressed with the CMV promoter bind the 10x[A] sequence of the second plasmid and over-express mCitrine.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In a second hand both plasmids, <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K782029">BBa_K782029</a> and <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K782065">BBa_K782065</a> were co-injected. The TALA VP16 expressed with the CMV promoter bind the 10x[A] sequence of the second plasmid and over-express mCitrine.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><img src="https://static.igem.org/mediawiki/2012/f/fc/Injection_mcitrineTeamSlovenia.png" width=450px></ins></div></td></tr>
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</table>TriCerhttp://2012.igem.org/wiki/index.php?title=Team:Evry/TeamSlovenia_collaboration&diff=291260&oldid=prevTriCer at 22:47, 26 October 20122012-10-26T22:47:56Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>To test this system we first injected embryos with the plasmid <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K782029">BBa_K782029</a> which will express few mCitrine.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>To test this system we first injected embryos with the plasmid <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K782029">BBa_K782029</a> which will express few mCitrine.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>In a second hand both plasmids,<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K782029">BBa_K782029</a> and <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K782065">BBa_K782065</a> were co-injected. The TALA VP16 expressed with the CMV promoter bind the 10x[A] sequence of the second plasmid and over-express mCitrine.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>In a second hand both plasmids, <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K782029">BBa_K782029</a> and <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K782065">BBa_K782065</a> were co-injected. The TALA VP16 expressed with the CMV promoter bind the 10x[A] sequence of the second plasmid and over-express mCitrine.</div></td></tr>
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</table>TriCer