Team:Evry/Tadpole injection1

From 2012.igem.org

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<li> pCS2+ citrine: contains the constitutive promoter CMV and the fluorescent protein citrin (yellow).</li><br/><br/>
<li> pCS2+ citrine: contains the constitutive promoter CMV and the fluorescent protein citrin (yellow).</li><br/><br/>
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<li> pCS2+ mCFP: contains the constitutive promoter CMV and the fluorescent protein mCFP(Cyan Fluorescent Protein).</li><br/><br/>
+
<li> pCS2+ mCFP: contains the constitutive promoter CMV and the fluorescent protein mCFP (Cyan Fluorescent Protein).</li><br/><br/>
<li> pCS2+ GFP-aid co injected with the plasmid pNHK60 (from Nishimura et al., 2009) : contains the constitutive promoter CMV and the aid sequenced of the aid system fusionned to GFP, pNHK60 contains the CMV promoter followed by the TIR1 protein which in presence of auxin degrade form a complex with aid of GFP-aid and degrade it <a href="http://2012.igem.org/Team:Evry/AIDSystem">AID system</a>.</li><br/><br/>
<li> pCS2+ GFP-aid co injected with the plasmid pNHK60 (from Nishimura et al., 2009) : contains the constitutive promoter CMV and the aid sequenced of the aid system fusionned to GFP, pNHK60 contains the CMV promoter followed by the TIR1 protein which in presence of auxin degrade form a complex with aid of GFP-aid and degrade it <a href="http://2012.igem.org/Team:Evry/AIDSystem">AID system</a>.</li><br/><br/>

Revision as of 22:06, 17 September 2012

Plasmid injection in fertilized Xenopus laevis eggs


Plasmid injected:



  1. pCS2+ GFP-aid: contains the constitutive promoter CMV and the aid sequenced of the aid system fusionned to GFP (Green Fluorescent Protein)(Nishimura et al., 2009).


  2. pCS2+ citrine: contains the constitutive promoter CMV and the fluorescent protein citrin (yellow).


  3. pCS2+ mCFP: contains the constitutive promoter CMV and the fluorescent protein mCFP (Cyan Fluorescent Protein).


  4. pCS2+ GFP-aid co injected with the plasmid pNHK60 (from Nishimura et al., 2009) : contains the constitutive promoter CMV and the aid sequenced of the aid system fusionned to GFP, pNHK60 contains the CMV promoter followed by the TIR1 protein which in presence of auxin degrade form a complex with aid of GFP-aid and degrade it AID system.


References:


Nishimura, K. et al., 2009. An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nature Methods, 6(12), p.917-922.