Team:Evry/PromoterReporter

From 2012.igem.org

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<td style="font-weight:bold; text-decoration:underline;">Excitation (nm)</td>
<td style="font-weight:bold; text-decoration:underline;">Excitation (nm)</td>
<td style="font-weight:bold; text-decoration:underline;">Emission (nm)</td>
<td style="font-weight:bold; text-decoration:underline;">Emission (nm)</td>
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</tr>
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<td>Red</td>
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<td>mCherry</td>
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<td>587</td>
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<td>610</td>
</tr>
</tr>
</table>
</table>

Revision as of 15:31, 18 September 2012

Reporter genes



Image-1 PR.png


Reporter genes are used as a toolbox in molecular biology to do gene expression, promoter or transformation/transfection assays. A reporter gene, to be introduced into an organism, is placed with the gene of interest in the same DNA construct. A good reporter confers easily identifiable characteristics as selectable markers.

Our interest was to perform promoter’s assays to visualize where our promoters allow the gene expression in the tadpoles with fluorescent proteins.






Image-2 PR.png


For our experiments, we used several fluorescent proteins (FPs) such mCitrine, CFP (cyan fluorescent protein), mCherry and sfGFP (superfolded green fluorescent protein) which are showed in the picture.


Class Protein Excitation (nm) Emission (nm)
Red mCherry 587 610


Image-3 PR.png

Image-4 PR.png

Image-5 PR.png