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From 2012.igem.org

Orthogonal hormonal system

Our orthogonal hormonal system is based on the use of auxin, a plant hormone. To implement this system, we used two different types of device corresponding to the production and the reception of auxin in the tadpole.

Auxin production devices

We envisaged 3 ways of design our auxin production device in tadpoles. The devices 1 and 2 have been designed to be expressed in tadpole's embryos, while device 3 have been designed to be expressed in E.coli. In this last case, the aim is that the tadpoles eat bacteria expressing device 3.

• Auxin production device 1 : this device is composed of BBa_K812???, coding for IaaM, and BBa_K812120, coding for IaaH for auxin generator, with a kozak sequence for the use in tadpole's embryos.
• Auxin production device 2 : this device is composed of BBa_K812014. It is ment for the co-expression of IaaH and IaaM gene in the same cell in tadpole.
• Auxin production device 3 : this device is composed of BBa_K515100, coding for IaaM and IaaH for auxin generator in E.coli.

Pathway

Auxin reception device

• Auxin reception device 1 : this device is composed of BBa_K812010 coding for GFP-AID, and BBa_K812012 coding for OsTir1.
• Auxin reception device 2 : this device is composed of BBa_K812013 coding for GFP-AID and OsTir1 in the same cell.