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| - | <h1>Orthogonal hormonal system</h1>
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| - | <h4><p>We adapted the auxin production device from the iGEM team Imperial college 2007 to eucaryotes and combined it with an auxin detection module. This way, we created the first synthetic hormonal system for intertissues communication.</p></h4>
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| - | <p>To test this system, we <a href="https://2012.igem.org/Team:Evry/InjectionTuto">co-injected</a> plasmids expressing our production and reception devices in tadpole's embryos, <a href="https://2012.igem.org/Team:Evry/FrenchFrog">a new chassis</a> we wanted to implement for synthetic biology. We performed auxin <a href="https://2012.igem.org/Team:Evry/AuxinTOX">toxicity</a> and <a href="https://2012.igem.org/Team:Evry/auxin_uptake">uptake</a> tests at the begining of our project to ensure the feasability.</p>
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| - | <h2>Auxin production devices</h2>
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| - | <p>We envisaged 3 ways of design our auxin production device in tadpoles. The devices 1 and 2 have been designed to be expressed in tadpole's embryos, while device 3 have been designed to be expressed in E.coli. In this last case, the aim is that <a href="https://2012.igem.org/Team:Evry/BXcom">the tadpoles eat bacteria</a> expressing device 3.</p>
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| - | <center>
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| - | <img src="https://static.igem.org/mediawiki/2012/9/97/Prodrecep.jpg" width="600px" alt="3 devices for production" />
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| - | </center>
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| - | <ul>
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| - | <li> <b>Auxin production device 1 :</b> this device is composed of <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812121">BBa_K812021</a>, coding for IaaM, and <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812120">BBa_K812120</a>, coding for IaaH for auxin generator for the use in tadpole's embryos.
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| - | <li> <b>Auxin production device 2 :</b> this device is composed of <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812014">BBa_K812014</a>. It is ment for the co-expression of IaaH and IaaM genes in the same cell in tadpole.
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| - | <li> <b>Auxin production device 3 :</b> this device is composed of <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K515100">BBa_K515100</a>, coding for IaaM and IaaH for auxin generator in <i>E.coli</i>.
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| - | </ul>
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| - | <center>
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| - | <img src="https://static.igem.org/mediawiki/2012/b/bd/ProductiondeviceCompress.jpg" width="900px" alt="Production devices" />
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| - | </center>
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| - | <h3>Pathway</h3>
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| - | <center>
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| - | <img src="https://static.igem.org/mediawiki/2012/4/4b/Pathway2.jpg" width="600px" alt="Auxin pathway" />
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| - | </center>
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| - | <h2>Auxin reception devices</h2>
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| - | <p>We envisaged 2 ways of design our auxin production device in tadpoles. To visualize the communication between different tissues or between E.coli and a tissue of the tadpole, we chose to work with GFP.Our orthogonal hormonal system <b>works with any proteins fused to AID signal</b> with any transcription factors.</p>
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| - | <ul>
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| - | <li> <b>Auxin reception device 1 :</b> this device is composed of <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812010">BBa_K812010</a> coding for GFP-AID, and <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812012">BBa_K812012</a> coding for OsTir1.
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| - | <li> <b>Auxin reception device 2 :</b> this device is composed of <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812013">BBa_K812013</a> coding for GFP-AID and OsTir1 in the same cell.
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| - | </ul></br>
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| - | <img src="https://static.igem.org/mediawiki/2012/f/f2/ReceptionCompress.jpg" width="930px" alt="devices for reception" />
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| - | <div id="citation_box">
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| - | <p id="references">References:</p>
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| - | <ol>
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| - | <li><i>1. Nishimura, K., Fukagawa, T., Takisawa, H., Kakimoto, T. & Kanemaki, M. An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nature methods 6, 917-22 (2009).</li>
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| - | </ol>
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| - | </div>
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