Team:Evry/OrthogonalSyst

From 2012.igem.org

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<p id="references">References:</p>
<p id="references">References:</p>
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<li><i>Inducible control of tissue-specific transgene expression in Xenopus tropicalis transgenic lines.</i>, Chae J., Zimmerman L.B., Grainger R.M., Mechanisms of development 117:1-2, 2002</li>
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<li><i>1. Nishimura, K., Fukagawa, T., Takisawa, H., Kakimoto, T. & Kanemaki, M. An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nature methods 6, 917-22 (2009).</li>
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<li><i>Xenopus: a prince among models for pronephric kidney development.</i>, Jones E., JASN 16:2, 2005</li>
 
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<li><i>An auxin-based degron system for the rapid depletion of proteins in nonplant cells</i>, Nishimura K., Fukagawa T., Takisawa H., Kakimoto T., Kanemaki M., Nature Methods 6:12, 2009</li>
 
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Revision as of 16:30, 23 September 2012

Orthogonal hormonal system

Our orthogonal hormonal system is based on the use of auxin, a plant hormone. To implement this system, we used two different types of device corresponding to the production and the reception of auxin in the tadpole.

Auxin production devices

We envisaged 3 ways of design our auxin production device in tadpoles. The devices 1 and 2 have been designed to be expressed in tadpole's embryos, while device 3 have been designed to be expressed in E.coli. In this last case, the aim is that the tadpoles eat bacteria expressing device 3.

3 devices for production
  • Auxin production device 1 : this device is composed of BBa_K812021, coding for IaaM, and BBa_K812120, coding for IaaH for auxin generator for the use in tadpole's embryos.
  • Auxin production device 2 : this device is composed of BBa_K812014. It is ment for the co-expression of IaaH and IaaM genes in the same cell in tadpole.
  • Auxin production device 3 : this device is composed of BBa_K515100, coding for IaaM and IaaH for auxin generator in E.coli.
Production devices

Pathway

Auxin pathway

Auxin reception device

We envisaged 2 ways of design our auxin production device in tadpoles.

  • Auxin reception device 1 : this device is composed of BBa_K812010 coding for GFP-AID, and BBa_K812012 coding for OsTir1.
  • Auxin reception device 2 : this device is composed of BBa_K812013 coding for GFP-AID and OsTir1 in the same cell.

devices for reception

Pathway

Production devices

References:

  1. 1. Nishimura, K., Fukagawa, T., Takisawa, H., Kakimoto, T. & Kanemaki, M. An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nature methods 6, 917-22 (2009).

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