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Team:Evry/OrthogonalSyst
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<h2>Auxin production devices</h2> | <h2>Auxin production devices</h2> | ||
<h3>Pathway</h3> | <h3>Pathway</h3> | ||
| - | In our system, the auxin (indole-3-acetic acid, IAA) is product from the precursor tryptophan. To ensure this pathway, IAA tryptophan monooxygenase (IaaM)catalyses the oxidative carboxylation of L-tryptophan to indole-3-acetamide which is hydrolyzed to IAA and ammonia by indoleacetamide hydrolase (IaaH). | + | <p>In our system, the auxin (indole-3-acetic acid, IAA) is product from the precursor tryptophan. To ensure this pathway, IAA tryptophan monooxygenase (IaaM)catalyses the oxidative carboxylation of L-tryptophan to indole-3-acetamide which is hydrolyzed to IAA and ammonia by indoleacetamide hydrolase (IaaH). </p> |
<h3>Devices</h3> | <h3>Devices</h3> | ||
| - | We envisaged 3 ways of design our auxin production device in tadpoles. The devices 1 and 2 have been designed to be expressed in tadpole's embryos, while device 3 have been designed to be expressed in E.coli. In this last case, the aim is that the tadpoles eat bacteria expressing device 3. | + | <p>We envisaged 3 ways of design our auxin production device in tadpoles. The devices 1 and 2 have been designed to be expressed in tadpole's embryos, while device 3 have been designed to be expressed in E.coli. In this last case, the aim is that the tadpoles eat bacteria expressing device 3.</p> |
<center> | <center> | ||
| - | <img src="https://static.igem.org/mediawiki/2012/ | + | <img src="https://static.igem.org/mediawiki/2012/b/ba/Production3devices.jpg" alt="3 devices for production" /> |
</center> | </center> | ||
<ul> | <ul> | ||
Revision as of 13:12, 23 September 2012
Orthogonal hormonal system
Our orthogonal hormonal system is based on the use of auxin, a plant hormone. To implement this system, we used two different type of device corresponding to the production and the reception of auxin in the tadpole.
Auxin production devices
Pathway
In our system, the auxin (indole-3-acetic acid, IAA) is product from the precursor tryptophan. To ensure this pathway, IAA tryptophan monooxygenase (IaaM)catalyses the oxidative carboxylation of L-tryptophan to indole-3-acetamide which is hydrolyzed to IAA and ammonia by indoleacetamide hydrolase (IaaH).
Devices
We envisaged 3 ways of design our auxin production device in tadpoles. The devices 1 and 2 have been designed to be expressed in tadpole's embryos, while device 3 have been designed to be expressed in E.coli. In this last case, the aim is that the tadpoles eat bacteria expressing device 3.
- Auxin production device 1 : this device is composed of BBa_K812???, coding for IaaM, and BBa_K812120 , coding for IaaH (Indoleacetamide hydrolyase). for auxin generator, with a kozak sequence for the use in tadpole's embryos.
- Auxin production device 2 : this device is composed of BBa_K812014. It is ment for the co-expression of IaaH and IaaM gene in the same cell in tadpole. This two devices have been designed to be expressed in
- Auxin production device 3 : this device is composed of BBa_K515100, coding for IaaM and IaaH.
Auxin reception device
