Team:Evry/Notebook/w8

From 2012.igem.org

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Digestion of psc2+ and mRFP with EcoRI and PstI.<br/>
Digestion of psc2+ and mRFP with EcoRI and PstI.<br/>
Ligation and Transformation on T10.  
Ligation and Transformation on T10.  
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<h3>TirI BB</h3>
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Electrophoresis of TirI last PCR: failure of this technique.<br>
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We buy a new primer to try another PCR technic.
<h2>Tuesday, 31st July</h2>
<h2>Tuesday, 31st July</h2>
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Electrophoresis of 27/07 results: Failure of second PCR</br>
Electrophoresis of 27/07 results: Failure of second PCR</br>
We restart the first step  
We restart the first step  
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<h2>Thursday, 2nd August</h2>
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<h3>Sequencing</h3>
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Send pCS2+ to sequencing.
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<h3>Issue</h3>
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We found that pCS2+ was badly made. We restart the construction of this plasmid from the beginning.
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Revision as of 08:03, 3 August 2012

Weeks

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22

Week 8: 30th July - 5th August

Monday, 30th July

Digestion - Ligation - Transformation:

Digestion of psc2+ and mRFP with EcoRI and PstI.
Ligation and Transformation on T10.

TirI BB

Electrophoresis of TirI last PCR: failure of this technique.
We buy a new primer to try another PCR technic.

Tuesday, 31st July

Inoculation:

Inoculation of pcs2+ (27.07) and repiquage of psc2+ with RFP (30.07)
Inoculation of psB1C3 with GFP-AID TirI: Electrophoresis of 27/07 results: Failure of second PCR
We restart the first step

Thursday, 2nd August

Sequencing

Send pCS2+ to sequencing.

Issue

We found that pCS2+ was badly made. We restart the construction of this plasmid from the beginning.