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	this work included standard pcr amplification, purification , plasmid digestion, ligation of vector-insert, transformation, miniprepping, and sequencing.</ul>		this work included standard pcr amplification, purification , plasmid digestion, ligation of vector-insert, transformation, miniprepping, and sequencing.</ul>
-	<li>Golden gate assemebly of GFP-AID-Tir1 cassette in Psblc3 and Pcs2+:	+	<li>Golden gate assemebly of GFP-AID-Tir1 cassette in Psblc3 and Pcs2+:</li>

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Revision as of 00:17, 27 September 2012

Weeks:

June			July				August				September				October				November

Week 14: 10th September - 16th September

this week we have worked extensively on a batch of different manipulations including:

• biobricking promotors in Psb1c3 which are:
  • P Elastase
  • Hsp70
  • P Car A
  • FLk1
  • P CMV
  this work included standard pcr amplification, purification , plasmid digestion, ligation of vector-insert, transformation, miniprepping, and sequencing.

Golden gate assemebly of GFP-AID-Tir1 cassette in Psblc3 and Pcs2+:

this included PCR amplification of seperate genes GFP-AID and Tir1 using then apllying the golden gate protocol over night, followed by trsnformtion, sequencing and characterization of those of PCS2+ in tadpoles

Preparation of chemical Competent Cells