Team:Evry/Notebook/w13

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Weeks:

June July August September October November

Week 13: 3rd September - 9th September

Monday 3rd

Construction about a new pCS2+ with RFP insert
-Digestion of the miniprep about pCSB1C3 with RFP insert by EcoRI and PstI -Purification gel to get only RFP insert ( gel image ) -Extraction of the RFP insert -Ligation with pCS2+

Tuesday 3rd

Preparation of a new stock of electrocompetent cells T10
Construction about a new pCS2+ with RFP insert :
Following -Transformation by electroporation with T10 cells -Plates prepared