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Team:Evry/Notebook/w13
From 2012.igem.org
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| - | -Digestion of the miniprep about pCSB1C3 with RFP insert by EcoRI and PstI | + | -Digestion of the miniprep about pCSB1C3 with RFP insert by EcoRI and PstI <br> |
| - | -Purification gel to get only RFP insert ( gel image ) | + | -Purification gel to get only RFP insert ( gel image )<br> |
| - | -Extraction of the RFP insert | + | -Extraction of the RFP insert <br> |
| Line 32: | Line 32: | ||
<u><h5>Construction about a new pCS2+ with RFP insert :</u></h5> | <u><h5>Construction about a new pCS2+ with RFP insert :</u></h5> | ||
| - | <i>Following</i> | + | <i>Following</i><br> |
| - | -Transformation by electroporation with T10 cells | + | -Transformation by electroporation with T10 cells<br> |
Revision as of 16:28, 5 September 2012
Weeks:
| June | July | August | September | October | November | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Week 13: 3rd September - 9th September
Monday 3rd
Construction about a new pCS2+ with RFP insert
-Digestion of the miniprep about pCSB1C3 with RFP insert by EcoRI and PstI-Purification gel to get only RFP insert ( gel image )
-Extraction of the RFP insert
-Ligation with pCS2+
Tuesday 3rd
Preparation of a new stock of electrocompetent cells T10
Construction about a new pCS2+ with RFP insert :
Following-Transformation by electroporation with T10 cells
-Plates prepared
