"
Team:Evry/Notebook/w13
From 2012.igem.org
(Difference between revisions)
PierreYves (Talk | contribs) |
|||
| (One intermediate revision not shown) | |||
| Line 21: | Line 21: | ||
-Ligation with pCS2+ | -Ligation with pCS2+ | ||
| + | <h5>Injection into tadpoles</h5> | ||
| - | + | Injection of plasmid pCS2+ with the CMV promoter and GFP-aid. | |
| + | This experiment was throughout the week. Taking pictures and take care of tadpole is a full-time work. | ||
<h2>Tuesday 3rd</h2> | <h2>Tuesday 3rd</h2> | ||
| Line 29: | Line 31: | ||
<u><h5>Preparation of a new stock of electrocompetent cells T10</u></h5> | <u><h5>Preparation of a new stock of electrocompetent cells T10</u></h5> | ||
| - | + | <br> | |
| + | <br> | ||
<u><h5>Construction about a new pCS2+ with RFP insert :</u></h5> | <u><h5>Construction about a new pCS2+ with RFP insert :</u></h5> | ||
| - | <i>Following</i><br> | + | <i>(Following)</i><br> |
Latest revision as of 03:06, 27 September 2012
Weeks:
| June | July | August | September | October | November | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Week 13: 3rd September - 9th September
Monday 3rd
Construction about a new pCS2+ with RFP insert
-Digestion of the miniprep about pCSB1C3 with RFP insert by EcoRI and PstI-Purification gel to get only RFP insert ( gel image )
-Extraction of the RFP insert
-Ligation with pCS2+
Injection into tadpoles
Injection of plasmid pCS2+ with the CMV promoter and GFP-aid. This experiment was throughout the week. Taking pictures and take care of tadpole is a full-time work.Tuesday 3rd
Preparation of a new stock of electrocompetent cells T10
Construction about a new pCS2+ with RFP insert :
(Following)-Transformation by electroporation with T10 cells
-Plates prepared
