Team:Evry/Notebook/w10

From 2012.igem.org

(Difference between revisions)
Line 6: Line 6:
<h2>Monday, 13th August</h2>
<h2>Monday, 13th August</h2>
<h3>Tadpoles injection (pNHK60)</h3>
<h3>Tadpoles injection (pNHK60)</h3>
-
<b>Preparation of dilution of pNHK60 (phenol/chloro extraction of Friday, 10th August)</b><br>
+
<b>Preparation of pNHK60 dilution(phenol/chloro extraction of Friday, 10th August)</b><br>
Tube A12 (50ng/ul): 1,695ul A11 + 73,305ul H2Odd<br>
Tube A12 (50ng/ul): 1,695ul A11 + 73,305ul H2Odd<br>
Tube A13 (25ng/ul): 25ul A12 + 25ul H2Odd<br>
Tube A13 (25ng/ul): 25ul A12 + 25ul H2Odd<br>

Revision as of 09:48, 13 August 2012

Weeks:

June July August September October November

Week 10: 13th August - 19th August

Monday, 13th August

Tadpoles injection (pNHK60)

Preparation of pNHK60 dilution(phenol/chloro extraction of Friday, 10th August)
Tube A12 (50ng/ul): 1,695ul A11 + 73,305ul H2Odd
Tube A13 (25ng/ul): 25ul A12 + 25ul H2Odd