"
Team:Evry/Notebook/June/20
From 2012.igem.org
(Created page with "Today's achievements: <i><b>Previous work :</b> liquid culture of DH5a with mRFP and auxin plasmid</i> Main goals : <br> °Auxin toxicity test <br> °Bacterial integration ass...") |
|||
| Line 29: | Line 29: | ||
Line D : RFP 0.1uL<br> | Line D : RFP 0.1uL<br> | ||
Line E : AUX 0.1uL<br> | Line E : AUX 0.1uL<br> | ||
| - | Line G & H : LB/MMR medium only (no embryos) | + | Line G & H : LB/MMR medium only (no embryos)<br> |
| - | + | https://static.igem.org/mediawiki/2012/8/8d/SNAP-175407-0003.jpg | |
| Line 42: | Line 42: | ||
<br> | <br> | ||
| + | We put this plate on UV light 15min after the deposit: | ||
| - | + | https://static.igem.org/mediawiki/2012/a/a9/Wt-0001.jpg | |
| Line 50: | Line 51: | ||
<center> | <center> | ||
| - | <a href="https://2012.igem.org/Team:Evry/Notebook/ | + | <a href="https://2012.igem.org/Team:Evry/Notebook/June/19"><img src="https://static.igem.org/mediawiki/2012/e/ed/Previous_day_arrow.png"></a> |
| - | <a href="https://2012.igem.org/Team:Evry/Notebook/ | + | <a href="https://2012.igem.org/Team:Evry/Notebook/June/21"><img src="https://static.igem.org/mediawiki/2012/e/e3/Next_day_arrow.png"></a> |
</center> | </center> | ||
</html> | </html> | ||
Revision as of 12:43, 10 July 2012
Today's achievements:
Previous work : liquid culture of DH5a with mRFP and auxin plasmid
Main goals :
°Auxin toxicity test
°Bacterial integration assessment into the xenopus embryos
1°/Xenopus embryos presents a protective membrane, we decided to realize our bacterial deposits experiments on xenopus with and without their membrane. Then, we could see if bacterias could enter into the embryos naturally.
2°/ On 96 plates :
Plate 1
each plate contain : 1 embryo
Line A & B = on embryos without protective membrane : 500 uL mRFP plasmid on DH5a deposite + 1mL LB + 1mL MMR medium
Line C : 1 2 3 : normal embryos : 500 uL mRFP plasmid on DH5a deposite + 1mL (LB + MMR medium)
Line E & F = embryos without protective membrane : 500 uL AUX plasmid integrated on DH5a deposit + 1mL LB + 1mL MMR medium
Line G & M1-6 = normal embryos : 500 uL AUX plasmid integrated on DH5a deposit + 1mL LB + 1mL MMR medium
Line M7-9 = normal embryos : controls
Plate 2
normal embryos only
Line A & B
Line C : LB/MMR medium
Line D : RFP 0.1uL
Line E : AUX 0.1uL
Line G & H : LB/MMR medium only (no embryos)
3°/ On 16 Plates
Tadpoles experimentations :
each plate contains 3 tadpoles
Line A : 0.5uL RFP bacteria
Line B1 : 0.3uL RFP bacteria
We put this plate on UV light 15min after the deposit:
