Latest revision as of 16:10, 12 July 2012

Promoters & Reporters workgroup

Digestion

Miniprep 28/06/2012

masse DNA = 2000 ng Volume final = 30 uL

DNA	Concentration (ng.uL-1)	V DNA (uL)	V SpeI (uL)	V EcoRI (uL)	V XBA I (uL)	V Buffer Orange (uL)	V Buffer sup (uL)	V water (uL)
pCS2 +	362,7	5,51	0	1	1	3	0	19,49
CFP	240,1	8,33	1	1	0	0	3	16,67
GFP	209,1	9,56	1	1	0	0	3	15,44
RFP	142,8	14,01	1	1	0	0	3	10,99
YFP	196,0	10,20	1	1	0	0	3	14,80

Incubation 3h at 37 degrees Celsius

Gel Migration

Gel at 0,08%
V tae = 30 mL
m aga = 0,24 g
V bet = 3 uL

No Band for RFP (again)

Gel Extraction

see protocol on 05/07/2012

Type	Gel weight (g)	V QG (uL)	V iso (uL)
pCS2 +	0,37	1 110	370
YFP	0,17	510	170
GFP	0,23	690	230
CFP	0,19	570	190

@@ Line 102: / Line 102: @@
    </TR>
    <TR>
-     <TH> pCS2 </TH>
+     <TH> pCS2 +</TH>
      <TD> <center>0,37</center></TD>
      <TD> <center>1 110</center></TD>
@@ Line 135: / Line 135: @@
    </TR>
    <TR>
-     <TH> pCS2 </TH>
+     <TH> pCS2 +</TH>
      <TD> <center>16,7</center></TD>
    </TR>