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	<FONT SIZE=3>'''Site Mutagenesis directed'''</FONT> <br/>		<FONT SIZE=3>'''Site Mutagenesis directed'''</FONT> <br/>
-	PCR for directed mutagenesis to replace 2 bases from TIR1 in order to remove 2 Restrictions Site </br>	+	PCR for directed mutagenesis to replace 2 bases from TIR1 in order to remove 2 Restrictions Site <br>
	''Primer used'' : SdMTIR1-Fw and SdMTIR1-Rv		''Primer used'' : SdMTIR1-Fw and SdMTIR1-Rv
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Revision as of 17:31, 20 July 2012

Reporter + TIR1 groups

'''PCR Colony'''

PCR colony of T10 pSC2+ with RFP
''Primers used'' : SP6 and T3


'''Site Mutagenesis directed'''
PCR for directed mutagenesis to replace 2 bases from TIR1 in order to remove 2 Restrictions Site
''Primer used'' : SdMTIR1-Fw and SdMTIR1-Rv