Team:Evry/Freezer

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Freezer


Primers


Number Name Sequence Length Tm  %GC Comments
P1 Tir1-BB-FW cgcttctagATGACGTACTTCCCGGAGG 19 57 58
P2 Tir1-BB-Rv ctactagtaTTATTATAGGATTTTAACAAAATTTGGTGC 24 52 29
P3 SdMTir1-BB-Fw AACTGAACTCACTACTGTACTTCTGTCACCAAATGACTAATGCTGCTCTAGTTACTGTCGCCAAG 65 72 43
P4 SdMTir1-BB-Rv TTGGACAGCCCAAGGATACTGCAACAAGTCCCTCCTCTGTCACAGCAGAATAACCAGCTACG 62 76 52
P5 Tir1 SdM v02-Fw tgcTgtgacagaggagggac 20 60 60
P6 Tir1 SdM v02-Fw ctagAgcagcattagtcatttgg 23 55 43
P7 pCS2+BB-Fw cgctgcagGAACTATAGTGAGTCGTATTACG 23 51 39
P8 pCS2+BB-Rv tgcatGAATTCGAATCGATGGGATCC 21 54 48
P9 GFP-AID-BB-Fw gaattcgcggccgcttctagagATGGCCACAACCATGGTG 40 74 58
P10 GFP-AID-BB-Rv tactagtagcggccgctgcagTTATTAAACCTTACGTTTCTTTTTAGGGAC 51 70 43
P11 p2 Hsp70-Fw ACTGTCGACCCCGTTTAGCAGGAAATAGCC 30 67 53 Heat Shock
P12 p2 Hsp70-Rv ACTAAGCTTATTTGCGCTCCTTACAGTTTGC 31 63 42 Heat Shock
P13 p2 Flk-1-Fw ACTGTCGACCTGGACTCTGGGCTAGGG 27 68 63 Vasculature
P14 p2 Flk-1-Rv TCAAAGCTTGTTGACGTTTAGTCCAGGACAG 31 64 45 Vasculature
P15 p2 ROSA-Fw ACTGTCGACTAGATGAAGGAGAGCC 25 61 52 Ubiquitous
P16 p2 ROSA-Rv GATAAGCTTGGATCCCCGCAAACGCAC 27 66 56 Ubiquitous
P17 p2 CarA-Fw ACTGTCGACGTCCCATACCAGTACAATGCAAT 32 66 47 muscles
P18 p2 CarA-Rv AGTAAGCTTGTGCTGTGATTGAATTGGCTG 30 63 43 muscles
P19 IaaH-BB-Fw gaattcgcggccgcttctagATGCGCGAAATGATTACGC 19 55 47
P20 IaaH-BB-Rv ctgcagcggccgctactagtaTTATTAGCCTTTTAACACTTTTTCG 25 52 28
P21 IaaM-BB-Fw gaattcgcggccgcttctagatgtttggaccggatttccc 20 56 50
P22 IaaM-BB-Rv ctgcagcggccgctactagtattattagtcccccagcgc 18 55 56
P23 AID-Fw cgcttctagGGAGCTGGTGCAGGCGCT 18 64 72
P24 Sp6 ATTTAGGTGACACTATA 17 41 27 for BB detection when sequenced
P25 T3 AATTAACCCTCACTAAAGGG 20 50 40 for BB detection when sequenced