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Team:Evry/Freezer
From 2012.igem.org
Freezer
Primers
| Number | Name | Sequence | Length | Tm | %GC | Comments |
| P1 | Tir1-BB-FW | cgcttctagATGACGTACTTCCCGGAGG | 19 | 57 | 58 | |
| P2 | Tir1-BB-Rv | ctactagtaTTATTATAGGATTTTAACAAAATTTGGTGC | 24 | 52 | 29 | |
| P3 | SdMTir1-BB-Fw | AACTGAACTCACTACTGTACTTCTGTCACCAAATGACTAATGCTGCTCTAGTTACTGTCGCCAAG | 65 | 72 | 43 | |
| P4 | SdMTir1-BB-Rv | TTGGACAGCCCAAGGATACTGCAACAAGTCCCTCCTCTGTCACAGCAGAATAACCAGCTACG | 62 | 76 | 52 | |
| P5 | Tir1 SdM v02-Fw | tgcTgtgacagaggagggac | 20 | 60 | 60 | |
| P6 | Tir1 SdM v02-Fw | ctagAgcagcattagtcatttgg | 23 | 55 | 43 | |
| P7 | pCS2+BB-Fw | cgctgcagGAACTATAGTGAGTCGTATTACG | 23 | 51 | 39 | |
| P8 | pCS2+BB-Rv | tgcatGAATTCGAATCGATGGGATCC | ||||
| P9 | GFP-AID-BB-Fw | gaattcgcggccgcttctagagATGGCCACAACCATGGTG | ||||
| P10 | GFP-AID-BB-Rv | tactagtagcggccgctgcagTTATTAAACCTTACGTTTCTTTTTAGGGAC | ||||
| P11 | p2 Hsp70-Fw | ACTGTCGACCCCGTTTAGCAGGAAATAGCC | ||||
| P12 | p2 Hsp70-Rv | ACTAAGCTTATTTGCGCTCCTTACAGTTTGC | ||||
| P13 | p2 Flk-1-Fw | ACTGTCGACCTGGACTCTGGGCTAGGG | ||||
| P14 | p2 Flk-1-Rv | TCAAAGCTTGTTGACGTTTAGTCCAGGACAG | ||||
| P15 | p2 ROSA-Fw | ACTGTCGACTAGATGAAGGAGAGCC | ||||
| P16 | p2 ROSA-Rv | GATAAGCTTGGATCCCCGCAAACGCAC | ||||
| P17 | p2 CarA-Fw | ACTGTCGACGTCCCATACCAGTACAATGCAAT | ||||
| P18 | p2 CarA-Rv | AGTAAGCTTGTGCTGTGATTGAATTGGCTG | ||||
| P19 | IaaH-BB-Fw | gaattcgcggccgcttctagATGCGCGAAATGATTACGC | ||||
| P20 | IaaH-BB-Rv | ctgcagcggccgctactagtaTTATTAGCCTTTTAACACTTTTTCG | ||||
| P21 | IaaM-BB-Fw | gaattcgcggccgcttctagatgtttggaccggatttccc | ||||
| P22 | IaaM-BB-Rv | ctgcagcggccgctactagtattattagtcccccagcgc | ||||
| P23 | AID-Fw | cgcttctagGGAGCTGGTGCAGGCGCT | ||||
| P24 | Sp6 | |||||
| P25 | T3 |
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