Team:Evry/Freezer

From 2012.igem.org

(Difference between revisions)
Line 412: Line 412:
<td width="10%"> Name
<td width="10%"> Name
-
</td><td width="25%"> Sequence
+
</td><td width="100"> Sequence
-
</td><td width="100"> Length
+
</td><td width="5%"> Length
</td><td width="5%"> Species of origin
</td><td width="5%"> Species of origin
</td><td width="5%"> Expression domain
</td><td width="5%"> Expression domain

Revision as of 08:51, 6 August 2012


Freezer


Primers


Number Name Sequence Length Tm  %GC Comments Date
P1 Tir1-BB-FW cgcttctagATGACGTACTTCCCGGAGG 19 57 58 13th july
P2 Tir1-BB-Rv ctactagtaTTATTATAGGATTTTAACAAAATTTGGTGC 24 52 29 13th july
P3 SdMTir1-BB-Fw AACTGAACTCACTACTGTACTTCTGTCACCAAATGACTAATGCTGCTCTAGTTACTGTCGCCAAG 65 72 43 13th july
P4 SdMTir1-BB-Rv TTGGACAGCCCAAGGATACTGCAACAAGTCCCTCCTCTGTCACAGCAGAATAACCAGCTACG 62 76 52 13th july
P5 Tir1 SdM v02-Fw tgcTgtgacagaggagggac 20 60 60 25th july
P6 Tir1 SdM v02-Fw ctagAgcagcattagtcatttgg 23 55 43 25th july
P7 pCS2+BB-Fw cgctgcagGAACTATAGTGAGTCGTATTACG 23 51 39 13th july
P8 pCS2+BB-Rv tgcatGAATTCGAATCGATGGGATCC 21 54 48 13th july
P9 GFP-AID-BB-Fw gaattcgcggccgcttctagagATGGCCACAACCATGGTG 40 74 58 20th july
P10 GFP-AID-BB-Rv tactagtagcggccgctgcagTTATTAAACCTTACGTTTCTTTTTAGGGAC 51 70 43 20th july
P11 p2 Hsp70-Fw ACTGTCGACCCCGTTTAGCAGGAAATAGCC 30 67 53 Heat Shock 20th july
P12 p2 Hsp70-Rv ACTAAGCTTATTTGCGCTCCTTACAGTTTGC 31 63 42 Heat Shock 20th july
P13 p2 Flk-1-Fw ACTGTCGACCTGGACTCTGGGCTAGGG 27 68 63 Vasculature 20th july
P14 p2 Flk-1-Rv TCAAAGCTTGTTGACGTTTAGTCCAGGACAG 31 64 45 Vasculature 20th july
P15 p2 ROSA-Fw ACTGTCGACTAGATGAAGGAGAGCC 25 61 52 Ubiquitous 20th july
P16 p2 ROSA-Rv GATAAGCTTGGATCCCCGCAAACGCAC 27 66 56 Ubiquitous 20th july
P17 p2 CarA-Fw ACTGTCGACGTCCCATACCAGTACAATGCAAT 32 66 47 muscles 20th july
P18 p2 CarA-Rv AGTAAGCTTGTGCTGTGATTGAATTGGCTG 30 63 43 muscles 20th july
P19 IaaH-BB-Fw gaattcgcggccgcttctagATGCGCGAAATGATTACGC 19 55 47 23th july
P20 IaaH-BB-Rv ctgcagcggccgctactagtaTTATTAGCCTTTTAACACTTTTTCG 25 52 28 23th july
P21 IaaM-BB-Fw gaattcgcggccgcttctagatgtttggaccggatttccc 20 56 50 23th july
P22 IaaM-BB-Rv ctgcagcggccgctactagtattattagtcccccagcgc 39 55/73 56/59 23th july
P23 AID-Fw cgcttctagGGAGCTGGTGCAGGCGCT 18 64 72 25th july
P24 Sp6 ATTTAGGTGACACTATA 17 41 27 for BB detection when sequenced NP
P25 T3 AATTAACCCTCACTAAAGGG 20 50 40 for BB detection when sequenced NP
P26 primer_sequencing_pCS2+_Fw CAAGTGTAGCGGTCACGCTG 20 59 60 13th july
P27 primer_sequencing_pCS2+_Rv GCGTAATCATGGTCATAGCTG 21 52 48 13th july
P28 GFP_AID_BB_Fw_v2 TGCATgaattcgcggccgcttctagagATGGCCACAACCATGGTG 45 75 56
P29 IaaH_BB_Fw_v2 TGCATgaattcgcggccgcttctagATGCGCGAAATGATTACGC 44 74 52
P30 IaaM_BB_Fw_v2 TGCATgaattcgcggccgcttctagatgtttggaccggatttccc 45 74 53
P31 IRES_BB_F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCGATCATTCAagagaTCCCC 50 53/74 50/52
P32 IRES_BB_R GTTTCTTCCTGCAGCGGCCGCTACTAGTAttgtggccatattatcatcg 49 51/72 40/49
P33 BBa_G1005 gtttcttcctgcagcggccgctactagta 29 67 55
P34 IaaM_Kozak_BB_F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGGTTCCAAACCATGCGCGAAATGATTACGC 60 55/76 46/52
P35 IaaH_Kozak_BB_F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGGTTCCAAACCatgtttggaccggatttcc 60 54/76 47/52


Promoteurs


Name Sequence Length Species of origin Expression domain Restriction sites (EcoRI, XbaI, SpeI, PtsI) Reference
p2 Xlurp tctgtgtctccaaagatgccccagtagctccccatcttattttcactgcacatgctttgtactgctgtcacttactgagctcagggacccactcacaatatacagtacacatagaaatttcacagtataaggctgattagtaattaatacagataattactacatggaattacagaaaccagtgcaattagcatcagaatttaataatcagccctgtagcatcagcttatatgacaggccaacctcattttctgcttgataatttgcaacaactcctacgcttagcttctcaacagctgctcagagcccactgagcatgtgagagtcacagacactttccaagatggtgaccccctgtgacaggtttgaagtcctggatcattgctgctattgacaagctgaaactttaggctggtgcaataagttcagtatataaaacattgcaattctagccataataatttttagggtttagttctcctttaaggtggggccttgctaaagatgacttatttgcacatttataacatctgtcttaaaatagcatcttataactcatatacaaaaaaggggttatattgctacctacatgtcactcagcattaaaaagttccatgttccatgtttaagaggacatatttacaccgtttcaaacagtgaaatattctcatcttgctcgttggcaagaatgaggttaagtttgcagtgttgaataaaagataaatagtgattagctaaatttttcggcaggtttcattgtgaaaaagatgcccataggtgaaaaaaactgtgcgcaacaaaaaaattaattgtcgcccttcgccttcaatgcatttactgaccttttggcgaaacgagtcacattcgcccatcactaaagataaattaagtagtacacttccttcagtcttaaagtgcatcaacccaatgcaagatcatattgagctagaaggggaaggaatgccatatcatggataacacagggagataaatgtatctcctgtttgtagtagaggtcctatccatcagtaaaatatgtagacctctgcttcctagtggtaattgttgtacgcctcaacatccgtttctcaactagcaaagctttctcaaagcttattaccacctggagattaagcacctagatctagttattttccttgtgattgtgatatacagtatatgggacatcctagttggtaatttgcactacaggggaacaaaatggcatattcaggaatatatcagaaagaaaagcacatgcaaatagtgacaaacaatgcactgttaaaaaacaaaaaaagagaggcaggcctgttactctaggaaattcacctttaagagggagggagatataatttactaatatcagttaatttatttgaaagagaaaaagtagtaaaccccccactctctttaatacagtataaaaaaatattaaacagattgtgaacacaattaatgtggtgttaattcatatttgtgaattggtgtacaaacattcattgattaatcaaagtgcaagttcttcaatagtgcaatcaatgtccatgattttaagattaattaattatcttaaaaaaattattcaattagttcaatttttaaaagctgtaccctgcttgtaatctaattaactaaattcatctaataagtggaattaccgagtgtaattacagaaggtagcaattatattgaattaacaatatcaaattgaattcattaatttaaagtgctagtgcaaatataattaattagaaggtgaattaattcaatgaaagttaaaaatgtatgtccaggaccaacatctgaaaaaatgtaggaaaaggaaaataatagaggtggagaggtcgcaaaaacttctataagaggtttttctatgccctgggactccacacaatggagaagacaaacactggggactgtggtctcaaattaaccgtatctatctcattgtggagaggctaagctgtactataaaaccacagatccttcagtgtcattgaaagtaaggagaatttaaaggaagagaaaatcaaatgcggcacttgaaaggctaacagcgctggatattaattaaaagaaccaagccaattctaaaaagggtttggtagagaacagatttgttaaaatcaatttgaaagttaggagactccgccaatgcactgtaataagaaaaagtatatcagtttgcaggaaaacggagaaggcaaaactgggtggctaaaagaagtgagaaaaagaaggactagaaatgaaggggtggaaacctgagtggtgtagtaaaaagcaacaataaataaaaaaacacaacatagtgaaaatattttattgccatctgtttattaaaatctgtctattctgcacctgcaattctaaaatcattgtattctgaacagctgatattgcattgctctgttggaacttgaatggcggccctgtggctatttaagcttaattctataaacaatattagaaactcgaaaatgaacacacagaatttaccagtagaggattaaagtttattttatgtaaatgaatacaaaattcttactgtttttggttgctattggccgtttagatattttttaagcaaaacattagcaaagccctcaaagttaataattcatcggttaggccgtcctttattgatccatcaatcaggattcagttttgtgctgtcaaaaacaatgtgcgtgggaacaactttatgtggagcagggactcttcatgctctggtcagaaaatgtacaggaacttggaacagagaagaagtataaagaaaataatagcatgcatcacaatgtttctggtgtgccaatgaatagctgtgtgttacaaaatctctgtcttgtataaagacaacaccagctacagtaagttaaattctaaacacatatttgtccaatacaggggcccaattaatactgcttgtgcttttacggcacaatcaatctttaagaaaacccctcaagagtttcctaactcacgctggttttcatgctgaaaaagaaagctttgtaagttacccgacctgctgcaggactttatcccagggtgtattaataggagtttttacaataatggctcatggtaatactctgattaaactcaatagctcattgcaatactttgataaagaactgtataaaaatataacacaaagaactgttctgctgttgaacccgatgtcgtatgttgagcccaatatagagtaaaaagacagctttataacgtataattgtgtataacaaagctttgtatgatagtatacaaggcagccttataatccataatcctgtataatgaagcgttgtgcaagagaaatcatgtattatctaatatgcttatgatttctgaagcccttatcagactcttctttattataaataggggcacaatatgctattcagctaccagctagctaagataagatctctgtttcggatgtgcttgtgggaaacaagatctgttattaaccaaatcattatgagttattgactaagcctctcaatgagcttactgtttgaaagcatatataaactgggggtcaccatttggtcagttgagatctcacttgtggggttgacgcgtgccgccacaggaccttccgtgctccgtttgagggattcaatctgctgactgttccaggatctcccagacttggttgcagattgtttgctttgctcttttactcattcggtaatgtatatcttcttcattctatgtatgtaataaacctttgcttatactagctggtgtgtttgaattacttccgagagactcatagaaccacaattaaaacacagggtaacaaagtctgggcacccctgagctgacatagggcatctattgtctttgtccacagtcctgatatttttttatcaagcagtaactatgggcatgcaatttgcagtggggaaaacaagaatttggattggtgctagaactgtgtgctgctgaacaacaatgaaaactgccatcttaattctgctgaactgacacagctattttcttttttaaatatgtctattagttaaggggttaaactttaaaatgatgtagagagtaatatcccgagacaatttgctattggtttttattttttttactatttatggtatttgagctatttcgctttttattcagcagctctccagtttgcaattctggcgatctggttattaggatccaatgggtcagtgacccctgtctgaaagctgaaaagcctcctaagaagtaggcaagtcatttaaaaaacgatataaaaaaataaaataatgaagaccaagtgaaaagttgcttagaagtggccaatctatatcatacttaaagggatattgtcatgggaaaaacatttttttcaaaatgaatcagttaatagtgctgctccatcagaattctgcactgaaatccatttctcaaaagagcaaacagatttttttatattcaattttgaaatttgacatggggctagacatattgtcaatttcccagctgccccaagtcatgtgacttgtgctctgataaactttaatcactctttactgctgtactgcaagttagagtgatatcaccccctccctttttccccacagcagccaaacaaaagaacaaagggaaggtaatcagatagcagctccttaacacaagataacagctgcctggttagatataagaacaacactcaatagtaaaaacccatgtcccactgagacacattcagttacattgagaaggaaaaacagcagcctgccagaaagcatttctctctaaagtgcaggcacaagtcacatgacttggggcagctgggaaattgacaaaatgtctagccccatgtcagatttcaaaattgaatataaaaaaatctgtttgctcttttgagaaatggatttcagtgcaaaattctgctggagaagcactattaactatttgaaaaaaacatgttttctgatgacagtatccctttaaagttgacttcatggtaaaccacccctttgaaaacattacagactgtcagaaaagtatgtataaatggggaaaatatcaggaccacctaatttgagatttggtaggtatgtcactgtttgttacttttttcggttagtgaccttgcaacatttcctgtaacagcttatttcagtgtttacccttgttgatcaagggttacacaaattcccagaaatataacgtgcctttagtgattgtgcaatccatatagataaaaggggatcttgctggcagagttcacagaagagctgacttagaaacccact 5273bp Xenopus Laevis Myeloid cells / Smith et al., 2002










Glycerol Stocks


Number Strain Plasmid Description Date
P1 E.Coli DH5 aplpha pCS2+ RFP ( BBA_J23100 ) 13th july
P2 E.Coli DH5 aplpha pCS2+ Auxine () 13th july
P3 E.Coli DH5 aplpha pCS2+ Tir1 GFP-Aid () 19th july
P4 E.Coli DH5 aplpha pCS2+ Tir1 GFP-Aid () 19th july