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Team:Evry/Freezer
From 2012.igem.org
(Difference between revisions)
| Line 52: | Line 52: | ||
<td style="background: #ccffcc;">P1 | <td style="background: #ccffcc;">P1 | ||
</td><td>Tir1-BB-FW | </td><td>Tir1-BB-FW | ||
| - | </td><td> | + | </td><td>cgcttctagATGACGTACTTCCCGGAGG |
</td><td>19 | </td><td>19 | ||
</td><td>57 | </td><td>57 | ||
| Line 61: | Line 61: | ||
<td style="background: #ccffcc;">P2 | <td style="background: #ccffcc;">P2 | ||
</td><td>Tir1-BB-Rv | </td><td>Tir1-BB-Rv | ||
| - | </td><td> | + | </td><td>ctactagtaTTATTATAGGATTTTAACAAAATTTGGTGC |
</td><td>24 | </td><td>24 | ||
</td><td>52 | </td><td>52 | ||
| Line 70: | Line 70: | ||
<td style="background: #ccffcc;">P3 | <td style="background: #ccffcc;">P3 | ||
</td><td>SdMTir1-BB-Fw | </td><td>SdMTir1-BB-Fw | ||
| - | </td><td> | + | </td><td>AACTGAACTCACTACTGTACTTCTGTCACCAAATGACTAATGCTGCTCTAGTTACTGTCGCCAAG |
</td><td>65 | </td><td>65 | ||
</td><td>72 | </td><td>72 | ||
| Line 79: | Line 79: | ||
<td style="background: #ccffcc;">P4 | <td style="background: #ccffcc;">P4 | ||
</td><td>SdMTir1-BB-Rv | </td><td>SdMTir1-BB-Rv | ||
| - | </td><td> | + | </td><td>TTGGACAGCCCAAGGATACTGCAACAAGTCCCTCCTCTGTCACAGCAGAATAACCAGCTACG |
</td><td>62 | </td><td>62 | ||
</td><td>76 | </td><td>76 | ||
| Line 88: | Line 88: | ||
<td style="background: #ccffcc;">P5 | <td style="background: #ccffcc;">P5 | ||
</td><td>Tir1 SdM v02-Fw | </td><td>Tir1 SdM v02-Fw | ||
| - | </td><td> | + | </td><td>tgcTgtgacagaggagggac |
</td><td>20 | </td><td>20 | ||
</td><td>60 | </td><td>60 | ||
| Line 97: | Line 97: | ||
<td style="background: #ccffcc;">P6 | <td style="background: #ccffcc;">P6 | ||
</td><td>Tir1 SdM v02-Fw | </td><td>Tir1 SdM v02-Fw | ||
| - | </td><td> | + | </td><td>ctagAgcagcattagtcatttgg |
</td><td>23 | </td><td>23 | ||
</td><td>55 | </td><td>55 | ||
| Line 106: | Line 106: | ||
<td style="background: #ccffcc;">P7 | <td style="background: #ccffcc;">P7 | ||
</td><td>pCS2+BB-Fw | </td><td>pCS2+BB-Fw | ||
| - | </td>< | + | </td><tdcgctgcagGAACTATAGTGAGTCGTATTACG |
</td><td>23 | </td><td>23 | ||
</td><td>51 | </td><td>51 | ||
| Line 115: | Line 115: | ||
<td style="background: #ccffcc;">P8 | <td style="background: #ccffcc;">P8 | ||
</td><td>pCS2+BB-Rv | </td><td>pCS2+BB-Rv | ||
| - | </td><td> | + | </td><td>tgcatGAATTCGAATCGATGGGATCC |
</td><td> | </td><td> | ||
</td><td> | </td><td> | ||
| Line 124: | Line 124: | ||
<td style="background: #ccffcc;">P9 | <td style="background: #ccffcc;">P9 | ||
</td><td>GFP-AID-BB-Fw | </td><td>GFP-AID-BB-Fw | ||
| - | </td><td> | + | </td><td>gaattcgcggccgcttctagagATGGCCACAACCATGGTG |
</td><td> | </td><td> | ||
</td><td> | </td><td> | ||
| Line 133: | Line 133: | ||
<td style="background: #ccffcc;">P10 | <td style="background: #ccffcc;">P10 | ||
</td><td>GFP-AID-BB-Rv | </td><td>GFP-AID-BB-Rv | ||
| - | </td><td> | + | </td><td>tactagtagcggccgctgcagTTATTAAACCTTACGTTTCTTTTTAGGGAC |
</td><td> | </td><td> | ||
</td><td> | </td><td> | ||
| Line 142: | Line 142: | ||
<td style="background: #ccffcc;">P11 | <td style="background: #ccffcc;">P11 | ||
</td><td>p2 Hsp70-Fw | </td><td>p2 Hsp70-Fw | ||
| - | </td><td> | + | </td><td>ACTGTCGACCCCGTTTAGCAGGAAATAGCC |
</td><td> | </td><td> | ||
</td><td> | </td><td> | ||
| Line 151: | Line 151: | ||
<td style="background: #ccffcc;">P12 | <td style="background: #ccffcc;">P12 | ||
</td><td>p2 Hsp70-Rv | </td><td>p2 Hsp70-Rv | ||
| - | </td><td> | + | </td><td>ACTAAGCTTATTTGCGCTCCTTACAGTTTGC |
</td><td> | </td><td> | ||
</td><td> | </td><td> | ||
| Line 160: | Line 160: | ||
<td style="background: #ccffcc;">P13 | <td style="background: #ccffcc;">P13 | ||
</td><td>p2 Flk-1-Fw | </td><td>p2 Flk-1-Fw | ||
| - | </td><td> | + | </td><td>ACTGTCGACCTGGACTCTGGGCTAGGG |
</td><td> | </td><td> | ||
</td><td> | </td><td> | ||
| Line 169: | Line 169: | ||
<td style="background: #ccffcc;">P14 | <td style="background: #ccffcc;">P14 | ||
</td><td>p2 Flk-1-Rv | </td><td>p2 Flk-1-Rv | ||
| - | </td><td> | + | </td><td>TCAAAGCTTGTTGACGTTTAGTCCAGGACAG |
</td><td> | </td><td> | ||
</td><td> | </td><td> | ||
| Line 178: | Line 178: | ||
<td style="background: #ccffcc;">P15 | <td style="background: #ccffcc;">P15 | ||
</td><td>p2 ROSA-Fw | </td><td>p2 ROSA-Fw | ||
| - | </td><td> | + | </td><td>ACTGTCGACTAGATGAAGGAGAGCC |
</td><td> | </td><td> | ||
</td><td> | </td><td> | ||
| Line 187: | Line 187: | ||
<td style="background: #ccffcc;">P16 | <td style="background: #ccffcc;">P16 | ||
</td><td>p2 ROSA-Rv | </td><td>p2 ROSA-Rv | ||
| - | </td><td> | + | </td><td>GATAAGCTTGGATCCCCGCAAACGCAC |
</td><td> | </td><td> | ||
</td><td> | </td><td> | ||
| Line 196: | Line 196: | ||
<td style="background: #ccffcc;">P17 | <td style="background: #ccffcc;">P17 | ||
</td><td>p2 CarA-Fw | </td><td>p2 CarA-Fw | ||
| - | </td><td> | + | </td><td>ACTGTCGACGTCCCATACCAGTACAATGCAAT |
</td><td> | </td><td> | ||
</td><td> | </td><td> | ||
| Line 205: | Line 205: | ||
<td style="background: #ccffcc;">P18 | <td style="background: #ccffcc;">P18 | ||
</td><td>p2 CarA-Rv | </td><td>p2 CarA-Rv | ||
| - | </td><td> | + | </td><td>AGTAAGCTTGTGCTGTGATTGAATTGGCTG |
</td><td> | </td><td> | ||
</td><td> | </td><td> | ||
| Line 214: | Line 214: | ||
<td style="background: #ccffcc;">P19 | <td style="background: #ccffcc;">P19 | ||
</td><td>IaaH-BB-Fw | </td><td>IaaH-BB-Fw | ||
| - | </td><td> | + | </td><td>gaattcgcggccgcttctagATGCGCGAAATGATTACGC |
</td><td> | </td><td> | ||
</td><td> | </td><td> | ||
| Line 223: | Line 223: | ||
<td style="background: #ccffcc;">P20 | <td style="background: #ccffcc;">P20 | ||
</td><td>IaaH-BB-Rv | </td><td>IaaH-BB-Rv | ||
| - | </td><td> | + | </td><td>ctgcagcggccgctactagtaTTATTAGCCTTTTAACACTTTTTCG |
</td><td> | </td><td> | ||
</td><td> | </td><td> | ||
| Line 232: | Line 232: | ||
<td style="background: #ccffcc;">P21 | <td style="background: #ccffcc;">P21 | ||
</td><td>IaaM-BB-Fw | </td><td>IaaM-BB-Fw | ||
| - | </td><td> | + | </td><td>gaattcgcggccgcttctagatgtttggaccggatttccc |
</td><td> | </td><td> | ||
</td><td> | </td><td> | ||
| Line 241: | Line 241: | ||
<td style="background: #ccffcc;">P22 | <td style="background: #ccffcc;">P22 | ||
</td><td>IaaM-BB-Rv | </td><td>IaaM-BB-Rv | ||
| - | </td><td> | + | </td><td>ctgcagcggccgctactagtattattagtcccccagcgc |
</td><td> | </td><td> | ||
</td><td> | </td><td> | ||
| Line 250: | Line 250: | ||
<td style="background: #ccffcc;">P23 | <td style="background: #ccffcc;">P23 | ||
</td><td>AID-Fw | </td><td>AID-Fw | ||
| - | </td><td> | + | </td><td>cgcttctagGGAGCTGGTGCAGGCGCT |
</td><td> | </td><td> | ||
</td><td> | </td><td> | ||
Revision as of 15:22, 1 August 2012
Freezer
Primers
| Number | Name | Sequence | Length | Tm | %GC | Comments |
| P1 | Tir1-BB-FW | cgcttctagATGACGTACTTCCCGGAGG | 19 | 57 | 58 | |
| P2 | Tir1-BB-Rv | ctactagtaTTATTATAGGATTTTAACAAAATTTGGTGC | 24 | 52 | 29 | |
| P3 | SdMTir1-BB-Fw | AACTGAACTCACTACTGTACTTCTGTCACCAAATGACTAATGCTGCTCTAGTTACTGTCGCCAAG | 65 | 72 | 43 | |
| P4 | SdMTir1-BB-Rv | TTGGACAGCCCAAGGATACTGCAACAAGTCCCTCCTCTGTCACAGCAGAATAACCAGCTACG | 62 | 76 | 52 | |
| P5 | Tir1 SdM v02-Fw | tgcTgtgacagaggagggac | 20 | 60 | 60 | |
| P6 | Tir1 SdM v02-Fw | ctagAgcagcattagtcatttgg | 23 | 55 | 43 | |
| P7 | pCS2+BB-Fw | <tdcgctgcagGAACTATAGTGAGTCGTATTACG </td><td>23 </td><td>51 </td><td>39 </td><td> <tr style="background: #cccccc;"> <td style="background: #ccffcc;">P8 </td><td>pCS2+BB-Rv </td><td>tgcatGAATTCGAATCGATGGGATCC </td><td> </td><td> </td><td> </td><td> <tr style="background: #cccccc;"> <td style="background: #ccffcc;">P9 </td><td>GFP-AID-BB-Fw </td><td>gaattcgcggccgcttctagagATGGCCACAACCATGGTG </td><td> </td><td> </td><td> </td><td> <tr style="background: #cccccc;"> <td style="background: #ccffcc;">P10 </td><td>GFP-AID-BB-Rv </td><td>tactagtagcggccgctgcagTTATTAAACCTTACGTTTCTTTTTAGGGAC </td><td> </td><td> </td><td> </td><td> <tr style="background: #cccccc;"> <td style="background: #ccffcc;">P11 </td><td>p2 Hsp70-Fw </td><td>ACTGTCGACCCCGTTTAGCAGGAAATAGCC </td><td> </td><td> </td><td> </td><td> <tr style="background: #cccccc;"> <td style="background: #ccffcc;">P12 </td><td>p2 Hsp70-Rv </td><td>ACTAAGCTTATTTGCGCTCCTTACAGTTTGC </td><td> </td><td> </td><td> </td><td> <tr style="background: #cccccc;"> <td style="background: #ccffcc;">P13 </td><td>p2 Flk-1-Fw </td><td>ACTGTCGACCTGGACTCTGGGCTAGGG </td><td> </td><td> </td><td> </td><td> <tr style="background: #cccccc;"> <td style="background: #ccffcc;">P14 </td><td>p2 Flk-1-Rv </td><td>TCAAAGCTTGTTGACGTTTAGTCCAGGACAG </td><td> </td><td> </td><td> </td><td> <tr style="background: #cccccc;"> <td style="background: #ccffcc;">P15 </td><td>p2 ROSA-Fw </td><td>ACTGTCGACTAGATGAAGGAGAGCC </td><td> </td><td> </td><td> </td><td> <tr style="background: #cccccc;"> <td style="background: #ccffcc;">P16 </td><td>p2 ROSA-Rv </td><td>GATAAGCTTGGATCCCCGCAAACGCAC </td><td> </td><td> </td><td> </td><td> <tr style="background: #cccccc;"> <td style="background: #ccffcc;">P17 </td><td>p2 CarA-Fw </td><td>ACTGTCGACGTCCCATACCAGTACAATGCAAT </td><td> </td><td> </td><td> </td><td> <tr style="background: #cccccc;"> <td style="background: #ccffcc;">P18 </td><td>p2 CarA-Rv </td><td>AGTAAGCTTGTGCTGTGATTGAATTGGCTG </td><td> </td><td> </td><td> </td><td> <tr style="background: #cccccc;"> <td style="background: #ccffcc;">P19 </td><td>IaaH-BB-Fw </td><td>gaattcgcggccgcttctagATGCGCGAAATGATTACGC </td><td> </td><td> </td><td> </td><td> <tr style="background: #cccccc;"> <td style="background: #ccffcc;">P20 </td><td>IaaH-BB-Rv </td><td>ctgcagcggccgctactagtaTTATTAGCCTTTTAACACTTTTTCG </td><td> </td><td> </td><td> </td><td> <tr style="background: #cccccc;"> <td style="background: #ccffcc;">P21 </td><td>IaaM-BB-Fw </td><td>gaattcgcggccgcttctagatgtttggaccggatttccc </td><td> </td><td> </td><td> </td><td> <tr style="background: #cccccc;"> <td style="background: #ccffcc;">P22 </td><td>IaaM-BB-Rv </td><td>ctgcagcggccgctactagtattattagtcccccagcgc </td><td> </td><td> </td><td> </td><td> <tr style="background: #cccccc;"> <td style="background: #ccffcc;">P23 </td><td>AID-Fw </td><td>cgcttctagGGAGCTGGTGCAGGCGCT </td><td> </td><td> </td><td> </td><td> <tr style="background: #cccccc;"> <td style="background: #ccffcc;">P24 </td><td>Sp6 </td><td> </td><td> </td><td> </td><td> </td><td> <tr style="background: #cccccc;"> <td style="background: #ccffcc;">P25 </td><td>T3 </td><td> </td><td> </td><td> </td><td> </td><td> </table>
