Team:Evry/Data

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Data page: Summary of what we have done this year (so far!)



New frog plasmids

Fig 1: Eucaryotic expression plasmid having both transcriptional and translational reagulational elements.

  • K812000: PCS2+ plasmid backbone for eukaryotic expression system containing regulational elements crucial for the efficacy of such system

The auxin system

The auxin receiver system

Fig 2: schemetic representation of the Auxin-receiver system

  • K812010:GFP fusion with the ubuquitinase E3 OsTirI recoginition domain which a main part of the degron system
  • K812012:OsTirI Ubiquitinase E3 for AID tagged protein degradation in the presence of auxin
  • K812013:

    The Auxin reciever system golden gate-assembled containing GFP-AID OsTirI polysistronic system for auxin detection in tadpole

The auxin emission system

Fig 3: Schematic representation of the Auxin two-step production cassette.

  • K812020:IAAH enzyme that catalyzes the second and last step towards auxin production
  • K812021:IAAM enzyme that catalyses the transformation of Tryptophan to Inodle-3-acetamide
  • K812014:Auxin production cassette containg both enzymes above which is monocystronic

Reporters and promotors

  • K812030:

    Characterization

    the information on characterized part can be found herein:
    • FrenchFrog:

      Goldenbricks

      Summary

      Fig 4: Summary of the GoldenBrick procedure

      The GoldenBrick is a new assembly method for the partsregistry. If you want to know more, see this page.

      Our favourites parts

      • K812050: A GoldenBricked version of pSB1C3 with J04450 as negative cloning control
      • K812051: A GoldenBricked version of pSB1K3 with J04450 as negative cloning control

      The other parts we have created

      • K812050: A GoldenBricked version of pSB1C3 with J04450 as negative cloning control
      • K812051: A GoldenBricked version of pSB1K3 with J04450 as negative cloning control
      • K812053: A GoldenBricked version of the strong RBS B0034
      • K812054: A GoldenBricked version of the RFP E1010
      • K812055: A GoldenBricked version of the terminator B0015
      • K812056: A GoldenBricked version of the pLac R0010 promoter
      • K812057: A GoldenBricked of an sfGFP protein
      • K812058: A GoldenBricked of medium strenght RBS J61107
      • K812059: A GoldenBricked of week RBS J61117

      say we are testing them